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    Intermediates and Methods for Forming Passivated Surfaces on Oxide Layers and Articles Produced Thereby
    3.
    发明申请
    Intermediates and Methods for Forming Passivated Surfaces on Oxide Layers and Articles Produced Thereby 有权
    中间体和氧化层上形成钝化表面的方法和由此产生的制品

    公开(公告)号：US20080176761A1

    公开(公告)日：2008-07-24

    申请号：US11943851

    申请日：2007-11-21

    申请人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    发明人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    IPC分类号： C40B50/14 ,  C40B40/00 ,  C07F9/09 ,  C07F9/02 ,  C40B50/18

    CPC分类号： G01N33/54393 ,  G01N33/551 ,  Y10T428/31678

    摘要： Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

    摘要翻译： 描述了用于在氧化物层上形成钝化表面的中间体和方法以及由此制备的制品。 氧化物表面上的羟基或氢氧基与式Y（L-POL）m的金属试剂反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒 或胺，“Pol”表示钝化剂，例如聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸酯官能团的钝化剂或包含钝化部分和多个与Y形成复合物的多个官能团的多价试剂反应。钝化剂还可以包括官能团 例如生物素，以提供具有所需功能的表面。 被钝化的表面表现出与生物分子的最小结合，并可用于单分子检测方案。

    Intermediates And Methods For Forming Passivated Surfaces On Oxide Layers And Articles Produced Thereby
    4.
    发明申请
    Intermediates And Methods For Forming Passivated Surfaces On Oxide Layers And Articles Produced Thereby 审中-公开
    中间体和方法在氧化物层和由此产生的制品上形成钝化表面

    公开(公告)号：US20110159305A1

    公开(公告)日：2011-06-30

    申请号：US13044759

    申请日：2011-03-10

    申请人： Steven M. MENCHEN ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    发明人： Steven M. MENCHEN ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    IPC分类号： B32B15/00

    CPC分类号： G01N33/54393 ,  G01N33/551 ,  Y10T428/31678

    摘要： Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

    摘要翻译： 描述了用于在氧化物层上形成钝化表面的中间体和方法以及由此制备的制品。 氧化物表面上的羟基或氢氧基与式Y（L-Pol）m的金属试剂反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol “表示钝化剂，例如聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸酯官能团的钝化剂或包含钝化部分和多个与Y形成复合物的多个官能团的多价试剂反应。钝化剂还可以包括官能团 例如生物素，以提供具有所需功能的表面。 被钝化的表面表现出与生物分子的最小结合，并可用于单分子检测方案。

    Fluorescent nucleobase conjugates having anionic linkers
    5.
    发明授权
    Fluorescent nucleobase conjugates having anionic linkers 有权
    具有阴离子接头的荧光核碱基缀合物

    公开(公告)号：US06811979B2

    公开(公告)日：2004-11-02

    申请号：US09976168

    申请日：2001-10-11

    申请人： Meng Taing ,  Shaheer H. Khan ,  Steven M. Menchen ,  Barnett B. Rosenblum

    发明人： Meng Taing ,  Shaheer H. Khan ,  Steven M. Menchen ,  Barnett B. Rosenblum

    IPC分类号： C12Q168

    CPC分类号： C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C40B40/00

    摘要： Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.

    摘要翻译： 提供的是核苷酸 - 染料缀合物和相关化合物，其中染料通过阴离子连接体直接或间接连接到核碱基。 连接体的阴离子特征由连接物中存在的一个或多个阴离子部分提供，例如磷酸酯，膦酸酯，磺酸酯和羧酸酯基团。 当染料作为供体/受体染料对提供时，阴离子连接体可以位于供体和受体之间，或位于核碱基与供体或受体之间或两者之间。 在一个实施方案中，本发明的缀合物为测序片段提供增强的电泳迁移率特性，例如使用标记的终止子进行双脱氧测序。

    Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers
    6.
    发明授权
    Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers 有权
    将金属氧化物沉积在表面上，作为羧化或磷化颗粒或聚合物的固定载体

    公开(公告)号：US08173198B2

    公开(公告)日：2012-05-08

    申请号：US12508539

    申请日：2009-07-23

    申请人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  George A. Fry

    发明人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  George A. Fry

    IPC分类号： G01N1/31 ,  C23C4/10 ,  C12N11/00 ,  A61B5/055 ,  C07H21/02

    CPC分类号： C07F9/6596 ,  C07F9/093 ,  C12Q1/6834 ,  Y10S530/81

    摘要： Intermediates and methods for forming activated metal complexes bound to surfaces on oxide layers, immobilizing beads to the modified surface and articles produced thereby are described. Hydroxyl groups on the oxide surfaces are reacted with a metal reagent complex of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a methoxyethanol, a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a plurality of functional groups that are reactive with or that form complexes with Y. The metal oxide surfaces exhibit minimal binding to bio-molecules, exhibit uniform deposition and immobilization of beads at high density, can be subsequently modified to create surfaces having a variety of properties, and can be used for nucleic acid sequencing and other analyses and in single-molecule detection schemes.

    摘要翻译： 描述了形成与氧化物层上的表面结合的活化金属配合物的中间体和方法，将珠子固定在改性表面上以及由此制备的制品。 氧化物表面上的羟基与式Y（L-Pol）m的金属试剂络合物反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol” 代表钝化剂如甲氧基乙醇，聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸盐官能团或与Y形成复合物的多个官能团的钝化剂反应。金属氧化物表面表现出与生物分子的最小结合，表现出均匀的沉积和 可以随后修饰珠子以高密度的固定，以产生具有多种性质的表面，并可用于核酸测序和其他分析以及单分子检测方案。

    Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby
    7.
    发明授权
    Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby 有权
    用于在由其制造的氧化物层和制品上形成钝化表面的中间体和方法

    公开(公告)号：US07928038B2

    公开(公告)日：2011-04-19

    申请号：US11943851

    申请日：2007-11-21

    申请人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    发明人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    IPC分类号： C40B60/14 ,  C12M1/34 ,  C07H21/02 ,  A61K38/04

    CPC分类号： G01N33/54393 ,  G01N33/551 ,  Y10T428/31678

    摘要： Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

    摘要翻译： 描述了用于在氧化物层上形成钝化表面的中间体和方法以及由此制备的制品。 氧化物表面上的羟基或氢氧基与式Y（L-Pol）m的金属试剂反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol “表示钝化剂，例如聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸酯官能团的钝化剂或包含钝化部分和多个与Y形成复合物的多个官能团的多价试剂反应。钝化剂还可以包括官能团 例如生物素，以提供具有所需功能的表面。 被钝化的表面表现出与生物分子的最小结合，并可用于单分子检测方案。

    Method and System for Phase-Locked Sequencing
    8.
    发明申请
    Method and System for Phase-Locked Sequencing 审中-公开
    锁相排序方法与系统

    公开(公告)号：US20070154921A1

    公开(公告)日：2007-07-05

    申请号：US11611339

    申请日：2006-12-15

    申请人： Timothy Woudenberg ,  Meng Taing

    发明人： Timothy Woudenberg ,  Meng Taing

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  G01J3/42

    CPC分类号： G01N21/6445 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6432 ,  C12Q2527/113 ,  C12Q2525/186 ,  C12Q2523/319

    摘要： System and methods according to exemplary embodiments of the present disclosure utilize a sample holder configured to hold at least one confined single-molecule analyte in a solution of labeled nucleotide bases. Each single-molecule analyte has a single template nucleic acid molecule, an oligonucleotide primer, and/or a single nucleic acid polymerizing enzyme. A least one light source is used to illuminate a detection volume around each confined analyte, and a pulsed source sends a pulsed radiation to the at least one detection volume. The timing of incorporation events at the analytes are controlled by the pulsed radiation, and when multiple analytes are provided on the sample holder, the incorporation events at the analytes can be phase locked and synchronized using the pulsed radiation.

    摘要翻译： 根据本公开的示例性实施方案的系统和方法使用样品保持器，其被配置为将至少一个限制的单分子分析物保持在标记的核苷酸碱基的溶液中。 每个单分子分析物具有单个模板核酸分子，寡核苷酸引物和/或单个核酸聚合酶。 至少一个光源用于照亮围绕每个受限分析物的检测体积，并且脉冲源将脉冲辐射发送到至少一个检测体积。 分析物上的掺入事件的时间由脉冲辐射控制，当样品架上提供多个分析物时，分析物上的结合事件可以使用脉冲辐射进行锁相和同步。