公开(公告)号：US07978403B2

公开(公告)日：2011-07-12

申请号：US12117334

申请日：2008-05-08

申请人： Steven R. J. Brueck ,  Alexander Neumann ,  Yuliya V. Kuznetsova

发明人： Steven R. J. Brueck ,  Alexander Neumann ,  Yuliya V. Kuznetsova

IPC分类号： G02B21/00

CPC分类号： G02B21/18 ,  G02B21/06 ,  G02B21/367

摘要： Exemplary embodiments provide an image interferometric microscope (IIM) and methods for image interferometric microscopy. The disclosed IIM can approach the linear systems limits of optical resolution by using a plurality of off-axis illuminations to access high spatial frequencies along with interferometric reintroduction of a zero-order reference beam on the low-NA side of the optical system. In some embodiments, a thin object can be placed normal to the optical axis and the frequency space limit can be extended to about [(1+NA)n/λ], where NA is the numerical-aperture of the objective lens used, n is the refraction index of the transmission medium and λ is an optical wavelength. In other embodiments, tilting the object plane can further allow collection of diffraction information up to the material transmission bandpass limited spatial frequency of about 2n/λ.

摘要翻译： 示例性实施例提供图像干涉显微镜（IIM）和用于图像干涉显微镜的方法。 所公开的IIM可以通过使用多个离轴照明来接近高分辨率的光学系统的光学分辨率的线性系统极限，以及在光学系统的低NA侧上的零级参考光束的干涉重新引入。 在一些实施例中，薄物体可以垂直于光轴放置，并且频率空间极限可以扩展到约[（1 + NA）n /λ]，其中NA是所用物镜的数值孔径，n 是传输介质的折射率，λ是光波长。 在其他实施例中，倾斜物平面可以进一步允许将衍射信息收集到材料透射带通限制空间频率约2n /λ。

公开(公告)号：US20090052019A1

公开(公告)日：2009-02-26

申请号：US12117334

申请日：2008-05-08

申请人： Steven R. J. BRUECK ,  Alexander Neumann ,  Yuliya V. Kuznetsova

发明人： Steven R. J. BRUECK ,  Alexander Neumann ,  Yuliya V. Kuznetsova

IPC分类号： G02B21/06

CPC分类号： G02B21/18 ,  G02B21/06 ,  G02B21/367

摘要： Exemplary embodiments provide an image interferometric microscope (IIM) and methods for image interferometric microscopy. The disclosed IIM can approach the linear systems limits of optical resolution by using a plurality of off-axis illuminations to access high spatial frequencies along with interferometric reintroduction of a zero-order reference beam on the low-NA side of the optical system. In some embodiments, a thin object can be placed normal to the optical axis and the frequency space limit can be extended to about [(1+NA)n/λ], where NA is the numerical-aperture of the objective lens used, n is the refraction index of the transmission medium and A is an optical wavelength. In other embodiments, tilting the object plane can further allow collection of diffraction information up to the material transmission bandpass limited spatial frequency of about 2n/λ.

摘要翻译： 示例性实施例提供图像干涉显微镜（IIM）和用于图像干涉显微镜的方法。 所公开的IIM可以通过使用多个离轴照明来接近高分辨率的光学系统的光学分辨率的线性系统极限，以及在光学系统的低NA侧上的零级参考光束的干涉重新引入。 在一些实施例中，薄物体可以垂直于光轴放置，并且频率空间极限可以扩展到约[（1 + NA）n /λ]，其中NA是所用物镜的数值孔径，n 是传输介质的折射率，A是光波长。 在其他实施例中，倾斜物平面可进一步允许将衍射信息收集到材料透射带通限制空间频率约为2n /λ。

公开(公告)号：US08203782B2

公开(公告)日：2012-06-19

申请号：US13087297

申请日：2011-04-14

申请人： Steven R. J. Brueck ,  Alexander Neumann ,  Yuliya V. Kuznetsova

发明人： Steven R. J. Brueck ,  Alexander Neumann ,  Yuliya V. Kuznetsova

IPC分类号： G02B21/00

CPC分类号： G02B21/18 ,  G02B21/06 ,  G02B21/367

摘要： Exemplary embodiments provide an image interferometric microscope (IIM) and methods for image interferometric microscopy. The disclosed IIM can approach the linear systems limits of optical resolution by using a plurality of off-axis illuminations to access high spatial frequencies along with interferometric reintroduction of a zero-order reference beam on the low-NA side of the optical system. In some embodiments, a thin object can be placed normal to the optical axis and the frequency space limit can be extended to about [(1+NA)n/λ], where NA is the numerical-aperture of the objective lens used, n is the refraction index of the transmission medium and λ is an optical wavelength. In other embodiments, tilting the object plane can further allow collection of diffraction information up to the material transmission bandpass limited spatial frequency of about 2n/λ.

摘要翻译： 示例性实施例提供图像干涉显微镜（IIM）和用于图像干涉显微镜的方法。 所公开的IIM可以通过使用多个离轴照明来接近高分辨率的光学系统的光学分辨率的线性系统极限，以及在光学系统的低NA侧上的零级参考光束的干涉重新引入。 在一些实施例中，薄物体可以垂直于光轴放置，并且频率空间极限可以扩展到约[（1 + NA）n /λ]，其中NA是所用物镜的数值孔径，n 是传输介质的折射率，λ是光波长。 在其他实施例中，倾斜物平面可以进一步允许将衍射信息收集到材料透射带通限制空间频率约2n /λ。

公开(公告)号：US20110211253A1

公开(公告)日：2011-09-01

申请号：US13087297

申请日：2011-04-14

申请人： Steven R. J. Brueck ,  Alexander Neumann ,  Yuliya V. Kuznetsova

发明人： Steven R. J. Brueck ,  Alexander Neumann ,  Yuliya V. Kuznetsova

IPC分类号： G02B21/06

CPC分类号： G02B21/18 ,  G02B21/06 ,  G02B21/367

摘要： Exemplary embodiments provide an image interferometric microscope (IIM) and methods for image interferometric microscopy. The disclosed IIM can approach the linear systems limits of optical resolution by using a plurality of off-axis illuminations to access high spatial frequencies along with interferometric reintroduction of a zero-order reference beam on the low-NA side of the optical system. In some embodiments, a thin object can be placed normal to the optical axis and the frequency space limit can be extended to about [(1+NA)n/λ], where NA is the numerical-aperture of the objective lens used, n is the refraction index of the transmission medium and λ is an optical wavelength. In other embodiments, tilting the object plane can further allow collection of diffraction information up to the material transmission bandpass limited spatial frequency of about 2n/λ.

摘要翻译： 示例性实施例提供图像干涉显微镜（IIM）和用于图像干涉显微镜的方法。 所公开的IIM可以通过使用多个离轴照明来接近高分辨率的光学系统的光学分辨率的线性系统极限，以及在光学系统的低NA侧上的零级参考光束的干涉重新引入。 在一些实施例中，薄物体可以垂直于光轴放置，并且频率空间极限可以扩展到约[（1 + NA）n /λ]，其中NA是所用物镜的数值孔径，n 是传输介质的折射率，λ是光波长。 在其他实施例中，倾斜物平面可以进一步允许将衍射信息收集到材料透射带通限制空间频率约2n /λ。

公开(公告)号：US10060904B1

公开(公告)日：2018-08-28

申请号：US14868128

申请日：2015-09-28

申请人： Steven R. J. Brueck ,  Yuliya Kuznetsova ,  Alexander Neumann

发明人： Steven R. J. Brueck ,  Yuliya Kuznetsova ,  Alexander Neumann

IPC分类号： G01N33/48 ,  G01N33/487 ,  B81C1/00 ,  G01N27/447 ,  C12Q1/68 ,  G03F7/20

CPC分类号： G01N33/48721 ,  B81C1/00071 ,  C12Q1/6869 ,  C12Q2565/631

摘要： In accordance with the disclosure, a method of forming a nanochannel is provided. The method includes depositing a photosensitive film stack over a substrate; forming a pattern on the film stack using interferometric lithography; depositing a plurality of silica nanoparticles to form a structure over the pattern; removing the pattern while retaining the structure formed by the plurality of silica nanoparticles, wherein the structure comprises one or more enclosed nanochannels, wherein each of the one or more nanochannels comprise one or more sidewalls and a roof; and partially sealing the roof of one or more nanochannels, wherein the roof comprises no more than one unsealed nanochannel per squared micron.

公开(公告)号：US09927397B1

公开(公告)日：2018-03-27

申请号：US14555453

申请日：2014-11-26

申请人： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza ,  C. Jeffrey Brinker

发明人： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza ,  C. Jeffrey Brinker

IPC分类号： G01N27/447 ,  C12Q1/68 ,  B01L3/00

CPC分类号： G01N27/44791 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2200/0663 ,  B01L2300/0645 ,  B01L2300/0896 ,  B01L2400/0421 ,  B81C1/00 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2563/157 ,  C12Q2563/159 ,  C12Q2565/631

摘要： Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US08526105B2

公开(公告)日：2013-09-03

申请号：US13345267

申请日：2012-01-06

申请人： Steven R. J. Brueck ,  Yuliya Kuznetsova ,  Alexander Neumann

发明人： Steven R. J. Brueck ,  Yuliya Kuznetsova ,  Alexander Neumann

IPC分类号： G02B21/06 ,  G02B21/00

CPC分类号： G02B21/0032 ,  G02B21/00 ,  G02B21/365 ,  G06T3/4061 ,  G06T2207/10056 ,  G06T2207/20056 ,  G06T2207/20221 ,  G06T2207/30148

摘要： In accordance with the invention, there are imaging interferometric microscopes and methods for imaging interferometric microscopy using structural illumination and evanescent coupling for the extension of imaging interferometric microscopy. Furthermore, there are coherent anti-Stokes Raman (CARS) microscopes and methods for coherent anti-Stokes Raman (CARS) microscopy, wherein imaging interferometric microscopy techniques are applied to get material dependent spectroscopic information.

摘要翻译： 根据本发明，存在成像干涉显微镜和用于使用结构照明和ev逝耦合来成像干涉显微镜的成像干涉显微镜成像的方法。 此外，还有相干的反斯托克斯拉曼（CARS）显微镜和相干反斯托克斯拉曼（CARS）显微镜的方法，其中应用成像干涉显微技术获得材料依赖的光谱信息。

公开(公告)号：US10969364B1

公开(公告)日：2021-04-06

申请号：US15896843

申请日：2018-02-14

申请人： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza ,  C. Jeffrey Brinker

发明人： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza ,  C. Jeffrey Brinker

IPC分类号： G01N27/447 ,  B01L3/00 ,  C12Q1/6869

摘要： Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US09156004B2

公开(公告)日：2015-10-13

申请号：US13831537

申请日：2013-03-14

申请人： Steven R. J. Brueck ,  Deying Xia ,  Yuliya Kuznetsova ,  Alexander Neumann

发明人： Steven R. J. Brueck ,  Deying Xia ,  Yuliya Kuznetsova ,  Alexander Neumann

IPC分类号： B01D69/04 ,  G03F7/20 ,  B81C1/00 ,  B82Y30/00

CPC分类号： B01D69/04 ,  B81C1/00071 ,  B82Y30/00 ,  G03F7/2051 ,  Y10S977/78 ,  Y10S977/84 ,  Y10T137/0324 ,  Y10T137/3006

摘要： In accordance with the invention, there is a method of forming a nanochannel including depositing a photosensitive film stack over a substrate and forming a pattern on the film stack using interferometric lithography. The method can further include depositing a plurality of silica nanoparticles to form a structure over the pattern and removing the pattern while retaining the structure formed by the plurality of silica nanoparticles, wherein the structure comprises an enclosed nanochannel.

摘要翻译： 根据本发明，存在一种形成纳米通道的方法，其包括在衬底上沉积感光膜堆叠并且使用干涉光刻在膜堆上形成图案。 该方法可以进一步包括沉积多个二氧化硅纳米颗粒以在图案上形成结构并除去图案，同时保留由多个二氧化硅纳米颗粒形成的结构，其中该结构包括封闭的纳米通道。

公开(公告)号：US09239455B2

公开(公告)日：2016-01-19

申请号：US13629598

申请日：2012-09-27

申请人： Steven R. J. Brueck ,  Alexander Neumann ,  Yuliya Kuznetsova

发明人： Steven R. J. Brueck ,  Alexander Neumann ,  Yuliya Kuznetsova

IPC分类号： G02B21/06 ,  G02B21/00 ,  G02B21/36 ,  G06T3/40 ,  G01N21/65

CPC分类号： G02B21/06 ,  G01N2021/653 ,  G02B21/0056 ,  G02B21/365 ,  G06T3/4061

摘要： In accordance with the aspects of the present disclosure, a method and apparatus is disclosed for imaging interferometric microscopy (IIM), which can use an immersion medium to enhance resolution up to a resolution of linear systems resolution limit of λ/4n, where λ is the wavelength in free space and n is the index of refraction of a transmission medium.

摘要翻译： 根据本公开的方面，公开了一种用于成像干涉显微镜（IIM）的方法和装置，其可以使用浸没介质来增强分辨率，直到λ/ 4n的线性系统分辨率极限的分辨率，其中λ是 自由空间中的波长和n是传输介质的折射率。