利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

41 条记录 (耗时 0.028 秒)

    AMINE-CONTAINING COMPOUND ANALYSIS METHODS
    1.
    发明申请
    AMINE-CONTAINING COMPOUND ANALYSIS METHODS 有权
    含胺化合物分析方法

    公开(公告)号：US20100093011A1

    公开(公告)日：2010-04-15

    申请号：US12555319

    申请日：2009-09-08

    申请人： Subodh B. NIMKAR ,  Subhasish Purkayastha ,  Darryl Pappin ,  Scott Daniels

    发明人： Subodh B. NIMKAR ,  Subhasish Purkayastha ,  Darryl Pappin ,  Scott Daniels

    IPC分类号： C12Q1/26 ,  G01N33/00 ,  G01N33/68

    CPC分类号： G01N33/6848 ,  Y10T436/142222 ,  Y10T436/173845 ,  Y10T436/24

    摘要： The present teachings provide methods for analyzing one or more amine-containing compounds in one or more samples using isobaric labels and parent-daughter ion transition monitoring (PDITM). In various embodiments, the methods comprise the steps of: (a) labeling one or more amine-containing compounds with different isobaric tags from a set of isobaric tags, each isobaric tag comprising a reporter ion portion; (b) combining at least a portion of each of the isobarically labeled amine-containing compounds to produce a combined sample; (c) subjecting at least a portion of the combined sample to PDITM; (d) measuring the ion signal of one or more of the transmitted reporter ions; and (e) determining the concentration of one or more of the isobarically labeled amine-containing compounds based at least on a comparison of the measured ion signal of the corresponding reporter ion to one or more measured ion signals of a standard compound.

    摘要翻译： 本教导提供了使用等压标记和亲 - 子离子跃迁监测（PDITM）在一个或多个样品中分析一种或多种含胺化合物的方法。 在各种实施方案中，所述方法包括以下步骤：（a）从一组等压标签组合标记具有不同等压标签的一种或多种含胺化合物，每个同量异位标签包含报告离子部分; （b）将每个等压标记的含胺化合物的至少一部分合并以产生组合的样品; （c）使组合样品的至少一部分经受PDITM; （d）测量一个或多个所传递的报道离子的离子信号; 至少基于相应报道离子的测量离子信号与标准化合物的一个或多个测量离子信号的比较来确定一种或多种等压标记的含胺化合物的浓度。

    Amine-containing compound analysis methods
    2.
    发明授权
    Amine-containing compound analysis methods 有权
    含胺化合物分析方法

    公开(公告)号：US08569071B2

    公开(公告)日：2013-10-29

    申请号：US12555319

    申请日：2009-09-08

    申请人： Subodh B. Nimkar ,  Subhasish Purkayastha ,  Darryl Pappin ,  Scott Daniels

    发明人： Subodh B. Nimkar ,  Subhasish Purkayastha ,  Darryl Pappin ,  Scott Daniels

    IPC分类号： G01N24/00

    CPC分类号： G01N33/6848 ,  Y10T436/142222 ,  Y10T436/173845 ,  Y10T436/24

    摘要： The present teachings provide methods for analyzing one or more amine-containing compounds in one or more samples using isobaric labels and parent-daughter ion transition monitoring (PDITM). In various embodiments, the methods comprise the steps of: (a) labeling one or more amine-containing compounds with different isobaric tags from a set of isobaric tags, each isobaric tag comprising a reporter ion portion; (b) combining at least a portion of each of the isobarically labeled amine-containing compounds to produce a combined sample; (c) subjecting at least a portion of the combined sample to PDITM; (d) measuring the ion signal of one or more of the transmitted reporter ions; and (e) determining the concentration of one or more of the isobarically labeled amine-containing compounds based at least on a comparison of the measured ion signal of the corresponding reporter ion to one or more measured ion signals of a standard compound.

    摘要翻译： 本教导提供了使用等压标记和亲 - 子离子跃迁监测（PDITM）在一个或多个样品中分析一种或多种含胺化合物的方法。 在各种实施方案中，所述方法包括以下步骤：（a）从一组等压标签组合标记具有不同等压标签的一种或多种含胺化合物，每个同量异位标签包含报告离子部分; （b）将每个等压标记的含胺化合物的至少一部分合并以产生组合的样品; （c）使组合样品的至少一部分经受PDITM; （d）测量一个或多个所传递的报道离子的离子信号; 至少基于相应报道离子的测量离子信号与标准化合物的一个或多个测量离子信号的比较来确定一种或多种等压标记的含胺化合物的浓度。

    Amine-containing compound analysis methods
    3.
    发明申请
    Amine-containing compound analysis methods 审中-公开

    公开(公告)号：US20060183238A1

    公开(公告)日：2006-08-17

    申请号：US11350147

    申请日：2006-02-08

    申请人： Subodh Nimkar ,  Subhasish Purkayastha ,  Darryl Pappin ,  Scott Daniels

    发明人： Subodh Nimkar ,  Subhasish Purkayastha ,  Darryl Pappin ,  Scott Daniels

    IPC分类号： G01N33/00

    CPC分类号： G01N33/6848 ,  Y10T436/142222 ,  Y10T436/173845 ,  Y10T436/24

    摘要： The present teachings provide methods for analyzing one or more amine-containing compounds in one or more samples using isobaric labels and parent-daughter ion transition monitoring (PDITM). In various embodiments, the methods comprise the steps of: (a) labeling one or more amine-containing compounds with different isobaric tags from a set of isobaric tags, each isobaric tag comprising a reporter ion portion; (b) combining at least a portion of each of the isobarically labeled amine-containing compounds to produce a combined sample; (c) subjecting at least a portion of the combined sample to PDITM; (d) measuring the ion signal of one or more of the transmitted reporter ions; and (e) determining the concentration of one or more of the isobarically labeled amine-containing compounds based at least on a comparison of the measured ion signal of the corresponding reporter ion to one or more measured ion signals of a standard compound.

    Quantitative analysis of vitamin D3, vitamin D2, and metabolites thereof
    10.
    发明授权
    Quantitative analysis of vitamin D3, vitamin D2, and metabolites thereof 有权
    维生素D3，维生素D2及其代谢物的定量分析

    公开(公告)号：US08617898B2

    公开(公告)日：2013-12-31

    申请号：US13013664

    申请日：2011-01-25

    申请人： Subhakar Dey ,  Sasi K. Pillai ,  Brian Williamson ,  Subhasish Purkayastha

    发明人： Subhakar Dey ,  Sasi K. Pillai ,  Brian Williamson ,  Subhasish Purkayastha

    IPC分类号： G01N33/00 ,  G01N30/72

    CPC分类号： G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

    摘要： Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled Diels Alder reagent.

    摘要翻译： 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的Diels Alder试剂的一步标记试剂。