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    METHOD OF PRODUCING A LIPOLYTIC ENZYME
    2.
    发明申请
    METHOD OF PRODUCING A LIPOLYTIC ENZYME 有权
    生产脂肪酶的方法

    公开(公告)号：US20120196002A1

    公开(公告)日：2012-08-02

    申请号：US13265712

    申请日：2010-04-23

    申请人： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

    发明人： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

    IPC分类号： A23L1/48 ,  C12N1/15 ,  C12N9/20

    CPC分类号： C12N9/20 ,  A23K20/189 ,  A23L29/06 ,  A23L33/14 ,  A23V2002/00 ,  C12N15/80 ,  C12Y301/01003

    摘要： The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.

    摘要翻译： 本发明提供转化或转染的里氏木霉细胞，其包含以下一种或多种：（i）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列（ ii）至少一个编码脂解酶的异源核苷酸序列，其中所述核苷酸序列包含如SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列，或（iii）至少一个编码脂解酶的异源核苷酸序列，其中 核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列; 并在允许表达编码所述脂肪分解酶的所述异源核苷酸序列的条件下培养细胞。

    Method of producing a lipolytic enzyme
    3.
    发明授权
    Method of producing a lipolytic enzyme 有权
    产生脂肪分解酶的方法

    公开(公告)号：US08940519B2

    公开(公告)日：2015-01-27

    申请号：US13265712

    申请日：2010-04-23

    申请人： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

    发明人： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

    IPC分类号： C12N1/20 ,  C12N9/20 ,  C12N15/00 ,  A23L1/03 ,  A23K1/165 ,  C12N15/80

    CPC分类号： C12N9/20 ,  A23K20/189 ,  A23L29/06 ,  A23L33/14 ,  A23V2002/00 ,  C12N15/80 ,  C12Y301/01003

    摘要： The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.

    摘要翻译： 本发明提供转化或转染的里氏木霉细胞，其包含以下一种或多种：（i）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列（ ii）至少一个编码脂解酶的异源核苷酸序列，其中所述核苷酸序列包含如SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列，或（iii）至少一个编码脂解酶的异源核苷酸序列，其中 核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列; 并在允许表达编码脂肪分解酶的异源核苷酸序列的条件下培养细胞。

    Cell culture compositions
    9.
    发明授权
    Cell culture compositions 有权
    细胞培养组合物

    公开(公告)号：US08674062B2

    公开(公告)日：2014-03-18

    申请号：US11938954

    申请日：2007-11-13

    申请人： Nigel Dunn-Coleman ,  Frits Goedegebuur ,  Michael Ward ,  Jian Yiao

    发明人： Nigel Dunn-Coleman ,  Frits Goedegebuur ,  Michael Ward ,  Jian Yiao

    IPC分类号： C07K7/08

    CPC分类号： C12N9/2437 ,  C11D3/38 ,  C11D3/38636 ,  C12Y302/01004 ,  Y02E50/16 ,  Y02E50/17

    摘要： The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6 (SEQ ID NO:1 encodes the full length endoglucanase; SEQ ID NO:4 encodes the mature form), and the corresponding endoglucanase VI amino acid sequence (“EGVI”; SEQ ID NO:3 is the signal sequence; SEQ ID NO:2 is the mature sequence). The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.

    摘要翻译： 本发明提供了一种命名为egl6（SEQ ID NO：1编码全长内切葡聚糖酶; SEQ ID NO：4编码成熟形式）的新型内切葡聚糖酶核酸序列和相应的内切葡聚糖酶VI氨基酸序列（“EGVI”; SEQ ID NO： ID NO：3是信号序列; SEQ ID NO：2是成熟序列）。 本发明还提供了包含编码EGVI的核酸序列，重组EGVI蛋白的表达载体和宿主细胞及其制备方法。