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公开(公告)号：US10301669B2
公开(公告)日：2019-05-28
申请号：US15469724
申请日：2017-03-27
Inventor： Moran Bercovici , Nadya Ostromohov , Ortal Schwartz
IPC: C12Q1/6825 , G01N27/447 , C12Q1/6816
Abstract: A method for sequence-specifically detecting a nucleic acid molecule. The method requires: a) contacting in an absence of an electric field, a mixture of nucleic acid molecules with a base pairing hybridizing molecule (BPHM) having a sequence of interest in a first solution and obtaining a hybrid consisting the nucleic acid molecule and the BPHM; b) introducing the first solution from step (a) into an ITP system, the ITP system comprises a second solution of high effective mobility leading electrolyte (LE) ions and a third solution of low effective mobility trailing electrolyte (TE); and c) applying the electric field across the second solution and the third solution. The hybrid focus at the sharp LE-TE interface in the ITP system. The TE has a higher mobility than the BPHM and the TE has a lower mobility than the hybrid. Sequence-specifically the detecting nucleic acid molecule by a signal from a label.
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公开(公告)号：US20150047982A1
公开(公告)日：2015-02-19
申请号：US14461645
申请日：2014-08-18
Inventor： Moran Bercovici , Nadya Ostromohov , Ortal Schwartz
IPC: G01N27/447
CPC classification number: C12Q1/6825 , C12Q1/6816 , G01N27/44726 , G01N27/4473 , G01N27/44765 , G01N27/44791 , C12Q2523/303 , C12Q2525/107 , C12Q2565/607
Abstract: The present invention provides a system including: a peptide nucleic acid (PNA) molecule; a DNA molecule, an RNA molecule, or a combination thereof; and an Isotachophoresis (ITP) system. Furthermore, the invention provides a method for sequence-specifically separating and/or identifying a nucleic acid molecule of interest by utilizing the system of the invention to separate and possibly label and/or detect a nucleic acid molecule of interest.
Abstract translation: 本发明提供一种系统，其包括：肽核酸（PNA）分子; DNA分子，RNA分子或其组合; 和等速电泳（ITP）系统。 此外，本发明提供了通过利用本发明的系统分离和可能标记和/或检测目的核酸分子来序列特异性分离和/或鉴定感兴趣的核酸分子的方法。
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公开(公告)号：US20170198339A1
公开(公告)日：2017-07-13
申请号：US15469724
申请日：2017-03-27
Inventor： Moran Bercovici , Nadya Ostromohov , Ortal Schwartz
IPC: C12Q1/68 , G01N27/447
CPC classification number: C12Q1/6825 , C12Q1/6816 , G01N27/44726 , G01N27/4473 , G01N27/44765 , G01N27/44791 , C12Q2523/303 , C12Q2525/107 , C12Q2565/607
Abstract: A method for sequence-specifically detecting a nucleic acid molecule. The method requires: a) contacting in an absence of an electric field, a mixture of nucleic acid molecules with a base pairing hybridizing molecule (BPHM) having a sequence of interest in a first solution and obtaining a hybrid consisting the nucleic acid molecule and the BPHM; b) introducing the first solution from step (a) into an ITP system, the ITP system comprises a second solution of high effective mobility leading electrolyte (LE) ions and a third solution of low effective mobility trailing electrolyte (TE); and c) applying the electric field across the second solution and the third solution. The hybrid focus at the sharp LE-TE interface in the ITP system. The TE has a higher mobility than the BPHM and the TE has a lower mobility than the hybrid. Sequence-specifically the detecting nucleic acid molecule by a signal from a label.
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公开(公告)号：US09683960B2
公开(公告)日：2017-06-20
申请号：US14461645
申请日：2014-08-18
Inventor： Moran Bercovici , Nadya Ostromohov , Ortal Schwartz
CPC classification number: C12Q1/6825 , C12Q1/6816 , G01N27/44726 , G01N27/4473 , G01N27/44765 , G01N27/44791 , C12Q2523/303 , C12Q2525/107 , C12Q2565/607
Abstract: The present invention provides a system including: a peptide nucleic acid (PNA) molecule; a DNA molecule, an RNA molecule, or a combination thereof; and an Isotachophoresis (ITP) system. Furthermore, the invention provides a method for sequence-specifically separating and/or identifying a nucleic acid molecule of interest by utilizing the system of the invention to separate and possibly label and/or detect a nucleic acid molecule of interest.
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