CONTINUOUS CELL DETECTION BY ISOTACHOPHORESIS

    公开(公告)号:US20180067077A1

    公开(公告)日:2018-03-08

    申请号:US15818837

    申请日:2017-11-21

    Abstract: A system comprising a protein and a channel. The channel has a domain that binds a membranal component. The channel is configured to carry a liquid sample to an isotachophoresis (ITP) apparatus. The liquid sample comprising or suspected of comprising a cell, a cell membrane or a fraction of a cell membrane. The ITP apparatus comprises a first zone and a second zone. The first zone is configured to contain a solution of high effective mobility leading electrolyte (LE) ion. The second zone is configured to contain a solution of low effective mobility trailing electrolyte (TE) ion. The first zone and the second zone are configured to be operably connected to at least one anode and at least one cathode.

    Detection of genetic sequences using PNA probes and Isotachophoresis

    公开(公告)号:US10301669B2

    公开(公告)日:2019-05-28

    申请号:US15469724

    申请日:2017-03-27

    Abstract: A method for sequence-specifically detecting a nucleic acid molecule. The method requires: a) contacting in an absence of an electric field, a mixture of nucleic acid molecules with a base pairing hybridizing molecule (BPHM) having a sequence of interest in a first solution and obtaining a hybrid consisting the nucleic acid molecule and the BPHM; b) introducing the first solution from step (a) into an ITP system, the ITP system comprises a second solution of high effective mobility leading electrolyte (LE) ions and a third solution of low effective mobility trailing electrolyte (TE); and c) applying the electric field across the second solution and the third solution. The hybrid focus at the sharp LE-TE interface in the ITP system. The TE has a higher mobility than the BPHM and the TE has a lower mobility than the hybrid. Sequence-specifically the detecting nucleic acid molecule by a signal from a label.

    Continuous cell detection by isotachophoresis

    公开(公告)号:US10527584B2

    公开(公告)日:2020-01-07

    申请号:US15818837

    申请日:2017-11-21

    Abstract: A system comprising a protein and a channel. The channel has a domain that binds a membranal component. The channel is configured to carry a liquid sample to an isotachophoresis (ITP) apparatus. The liquid sample comprising or suspected of comprising a cell, a cell membrane or a fraction of a cell membrane. The ITP apparatus comprises a first zone and a second zone. The first zone is configured to contain a solution of high effective mobility leading electrolyte (LE) ion. The second zone is configured to contain a solution of low effective mobility trailing electrolyte (TE) ion. The first zone and the second zone are configured to be operably connected to at least one anode and at least one cathode.

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