公开(公告)号：US20150064141A1

公开(公告)日：2015-03-05

申请号：US14390738

申请日：2013-03-14

Applicant: The Regents of the University of California

Inventor： James A. Byrne

IPC: A61K35/28 ,  G01N33/569

CPC classification number: A61K35/28 ,  C12N5/0668 ,  G01N33/56966 ,  G01N2333/70596

Abstract: Disclosed herein are three types of skin-derived cells. One type of the cells is mesenchymal stem cells characterized by expression of the cell surface biomarker cluster of differentiation (CD) 146, the second type expresses CD271. The third type of cells is regeneration-associated cells characterized by expression of the cell surface biomarkers stage-specific embryonic antigen 3 (SSEA3) and CD 105 (clone 35). Also disclosed are methods of isolating, purifying, culturing, storing, and using these cells.

Abstract translation: 本文公开了三种类型的皮肤衍生细胞。 一种类型的细胞是以细胞表面生物标志物分化簇（CD）146表达为特征的间充质干细胞，第二种类型表达CD271。 第三类细胞是以细胞表面生物标志物阶段特异性胚胎抗原3（SSEA3）和CD105（克隆35）的表达为特征的再生相关细胞。 还公开了分离，纯化，培养，储存和使用这些细胞的方法。

公开(公告)号：US20140178346A1

公开(公告)日：2014-06-26

申请号：US14140420

申请日：2013-12-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： James A. Byrne ,  Jeffrey C. Wang

IPC: A61K35/32

CPC classification number: A61K35/32 ,  A61K35/12 ,  A61K35/33 ,  A61K35/35 ,  A61K38/1875 ,  A61K38/39 ,  A61L24/0005 ,  A61L24/102 ,  A61L27/24 ,  A61L27/3633 ,  A61L27/3804 ,  A61L2300/64 ,  A61L2400/06 ,  A61L2430/02 ,  A61L2430/38 ,  C12N5/0656 ,  C12N2510/00 ,  C12N2511/00 ,  C12N2533/54 ,  A61K2300/00

Abstract: Cell compositions for tissue engineering are provided which contain a population of autologous, minimally passaged dermal fibroblasts in combination with a tissue engineering matrix or scaffold, or material forming a matrix or scaffold. In one embodiment, the population of fibroblasts is genetically engineered to secrete a therapeutic protein in an amount effective to induce tissue growth or tissue repair when the cell composition is transplanted into a subject in need thereof. For example, the therapeutic protein can be a bone morphogenic protein when the tissue to be treated is bone tissue. A preferred bone morphogenic protein is BMP-2.

Abstract translation: 提供用于组织工程的细胞组合物，其含有与组织工程基质或支架组合的自体，最少传代的真皮成纤维细胞群体或形成基质或支架的材料。 在一个实施方案中，当将细胞组合物移植到有需要的受试者中时，成纤维细胞群被遗传工程化以分泌有效诱导组织生长或组织修复的量的治疗性蛋白质。 例如，当待治疗的组织是骨组织时，治疗性蛋白质可以是骨形态发生蛋白质。 优选的骨形态发生蛋白是BMP-2。

公开(公告)号：US20150105343A1

公开(公告)日：2015-04-16

申请号：US14516451

申请日：2014-10-16

Applicant: The Regents of the University of California

Inventor： James A. Byrne ,  Caius Radu ,  Patrick Lee

IPC: C12N5/074 ,  A61K31/7064

CPC classification number: C12N5/0696 ,  A61K31/7064 ,  C12N2500/40 ,  C12N2500/98

Abstract: In various embodiments, a cell culture medium, or a nucleoside cocktail transmission (NCT) medium for the culture of stem cells with improved genetic stability, cellular function and regeneration ability is provided. Illustrative culture media comprise a basal culture medium for stem cells, where the culture medium is supplemented with one or more nucleoside triphosphates (e.g., dNTPs and/or NPs) or one or more precursors thereof. Illustrative NCT media comprise a delivery vehicle (e g. skin creams or other vehicle) containing one or more nucleoside triphosphates (e.g., dNTPs and/or NPs) or one or more precursors thereof. The NCT medium provides, inter alia, direct delivery of nucleoside cocktails into human tissues (such as skin) for regenerative, cosmetic and/or therapeutic purposes.

Abstract translation: 在各种实施方案中，提供了具有改善的遗传稳定性，细胞功能和再生能力的用于培养干细胞的细胞培养基或核苷混合物传播（NCT）培养基。 说明性的培养基包括用于干细胞的基础培养基，其中培养基补充有一种或多种核苷三磷酸（例如dNTP和/或NP）或其一种或多种前体。 说明性NCT培养基包含含有一种或多种核苷三磷酸（例如dNTP和/或NP）或其一种或多种前体的递送载体（例如护肤霜或其它载体）。 NCT培养基特别提供将核苷鸡尾酒直接递送到人体组织（如皮肤）中用于再生，化妆和/或治疗目的。