公开(公告)号：US09702795B2

公开(公告)日：2017-07-11

申请号：US14372277

申请日：2013-01-14

Applicant: THE SCRIPPS RESEARCH INSTITUTE ,  ENGINEERING ARTS LLC ,  Bridget Carragher ,  Clinton S. Potter ,  Tilak Jain ,  Peter Kahn ,  Peter Wiktor

Inventor： Bridget Carragher ,  Clinton S. Potter ,  Tilak Jain ,  Peter Kahn ,  Peter Wiktor

IPC: G01N1/28 ,  G01N1/42 ,  H01J37/26

CPC classification number: G01N1/28 ,  G01N1/2813 ,  G01N1/42 ,  H01J37/26 ,  H01J37/261 ,  H01J2237/2001 ,  H01J2237/2802

Abstract: The invention provides methods and devices for preparing frozen vitrified samples for transmission electron microscopy. By reducing the volume of sample from microliter scale to picoliter scale, the requirement for blotting of excess fluid is minimized or eliminated.

公开(公告)号：US09594008B2

公开(公告)日：2017-03-14

申请号：US14372274

申请日：2013-01-14

Applicant: THE SCRIPPS RESEARCH INSTITUTE ,  Bridget Carragher ,  Clinton S. Potter ,  Tilak Jain

Inventor： Bridget Carragher ,  Clinton S. Potter ,  Tilak Jain

IPC: G01N1/28 ,  G01N1/30 ,  G01N1/31 ,  H01J37/20 ,  H01J37/26

CPC classification number: G01N1/28 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  H01J37/20 ,  H01J37/261 ,  H01J2237/20 ,  H01J2237/2007 ,  H01J2237/202 ,  H01J2237/204 ,  H01J2237/208 ,  H01J2237/2602

Abstract: The invention provides methods and compositions for preparation of complex specimen arrays for analysis by electron microscopy. These methods and compositions can permit high throughput screening of samples on single EM grid supports using sample volumes in the nanoliter and picoliter range.

Abstract translation: 本发明提供用于制备用于通过电子显微镜分析的复合样品阵列的方法和组合物。 这些方法和组合物可以允许使用纳升速度和皮升范围内的样品体积在单EM网格支架上进行高通量筛选。

公开(公告)号：US20150090899A1

公开(公告)日：2015-04-02

申请号：US14372274

申请日：2013-01-14

Applicant: THE SCRIPPS RESEARCH INSTITUTE

Inventor： Bridget Carragher ,  Clinton S. Potter ,  Tilak Jain

IPC: G01N1/28 ,  H01J37/20 ,  H01J37/26

CPC classification number: G01N1/28 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  H01J37/20 ,  H01J37/261 ,  H01J2237/20 ,  H01J2237/2007 ,  H01J2237/202 ,  H01J2237/204 ,  H01J2237/208 ,  H01J2237/2602

Abstract: The invention provides methods and compositions for preparation of complex specimen arrays for analysis by electron microscopy. These methods and compositions can permit high throughput screening of samples on single EM grid supports using sample volumes in the nanoliter and picoliter range.

Abstract translation: 本发明提供用于制备用于通过电子显微镜分析的复合样品阵列的方法和组合物。 这些方法和组合物可以允许使用纳升速度和皮升范围内的样品体积在单EM网格支架上进行高通量筛选。

公开(公告)号：US20140360286A1

公开(公告)日：2014-12-11

申请号：US14372277

申请日：2013-01-14

Applicant: THE SCRIPPS RESEARCH INSTITUTE ,  ENGINEERING ARTS LLC

Inventor： Bridget Carragher ,  Clinton S. Potter ,  Tilak Jain ,  Peter Kahn ,  Peter Wiktor

IPC: G01N1/28 ,  G01N1/42 ,  H01J37/26

CPC classification number: G01N1/28 ,  G01N1/2813 ,  G01N1/42 ,  H01J37/26 ,  H01J37/261 ,  H01J2237/2001 ,  H01J2237/2802

Abstract: The invention provides methods and devices for preparing frozen vitrified samples for transmission electron microscopy. By reducing the volume of sample from microliter scale to picoliter scale, the requirement for blotting of excess fluid is minimized or eliminated.

Abstract translation: 本发明提供用于制备用于透射电子显微镜的冷冻玻璃化样品的方法和装置。 通过将样品从微升级的体积减小到皮升级，可以将过量流体的印迹要求降至最低或消除。

公开(公告)号：US10241011B2

公开(公告)日：2019-03-26

申请号：US15646701

申请日：2017-07-11

Applicant: The Scripps Research Institute ,  Engineering Arts LLC

Inventor： Bridget Carragher ,  Clinton S. Potter ,  Tilak Jain ,  Peter Kahn ,  Peter Wiktor

IPC: G01N1/28 ,  G01N1/42 ,  H01J37/26

Abstract: The invention provides methods and devices for preparing frozen vitrified samples for transmission electron microscopy. By reducing the volume of sample from microliter scale to picoliter scale, the requirement for blotting of excess fluid is minimized or eliminated.

公开(公告)号：US09952128B2

公开(公告)日：2018-04-24

申请号：US15285070

申请日：2016-10-04

Applicant: The Scripps Research Institute

Inventor： Bridget Carragher ,  Clinton S. Potter ,  Tilak Jain

IPC: G01N1/28 ,  G01N1/30 ,  G01N1/31 ,  H01J37/20 ,  H01J37/26

CPC classification number: G01N1/28 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  H01J37/20 ,  H01J37/261 ,  H01J2237/20 ,  H01J2237/2007 ,  H01J2237/202 ,  H01J2237/204 ,  H01J2237/208 ,  H01J2237/2602

Abstract: The invention provides systems or apparatuses for dispensing aqueous materials for electron microscopy (EM). The systems allow dispensing of aqueous materials onto an EM sample grid at individual specimen locations in an ordered array of specimen locations, with each individual specimen location in the array of locations. The systems contain a holder for reversibly receiving an EM sample grid, and a dispenser containing one or more dispensing elements that are configured to discretely dispense one or more aqueous solutions from the dispensing elements onto a plurality of individual specimen locations. The dispenser is able to provide an ordered array of discrete specimen locations discontinuous with one another. In the systems, at least one dispensing element is configured to dispense picoliter volumes of one or more of the aqueous solutions. Additionally, the systems contain a drive mechanism to position the EM sample grid relative to the one or more dispensing elements, as well as one or more reservoirs operably linked to the dispenser for holding the one or more aqueous solutions to be discretely dispensed onto each individual specimen location in the array of locations.