公开(公告)号：US20150225686A1

公开(公告)日：2015-08-13

申请号：US14620306

申请日：2015-02-12

Applicant: TOKYO ELECTRON LIMITED

Inventor： Yasuhiro OSHIMA ,  Shinichi GOMI ,  Kunitada HATABAYASHI ,  Kentarou TOMITA ,  Tomoaki KURAKAZU

IPC: C12M1/00 ,  C12N5/074 ,  C12N13/00

CPC classification number: C12M31/02 ,  C12M23/22 ,  C12M33/00 ,  C12N5/0696

Abstract: A method for detaching cells from a cell culture surface includes irradiating visible light in an irradiation amount of 4 J or greater per 1 mm2 to a cell adhesion surface to which cells are adhering on a substrate such that the cells are detached from the cell adhesion surface on the substrate.

Abstract translation: 用于从细胞培养表面分离细胞的方法包括将每1mm 2的4J以上的照射量的可见光照射到细胞粘附在基板上的细胞粘附面，使得细胞从细胞粘附面剥离 在基板上。

公开(公告)号：US20190119650A1

公开(公告)日：2019-04-25

申请号：US15769784

申请日：2016-03-16

Applicant: SHIMADZU CORPORATION ,  TOKYO ELECTRON LIMITED

Inventor： Takashi SUZUKI ,  Daisuke HIRAMARU ,  Masatoshi TAKAHASHI ,  Kentaro TOMITA ,  Kunitada HATABAYASHI ,  Kenichi KAGAWA ,  Shigenori OZAKI

IPC: C12N5/074 ,  G01N33/50

Abstract: The present invention is a method in which stem cells for which a differentiation state is unknown or cells that are differentiation-induced from the stem cells are use as test cells, and a differentiation state of the test cells is evaluated based on an abundance of a predetermined indicator substance in a culture supernatant of the test cells. The indicator substance is at least one compound selected from a group that consists of ornithine, 2-aminoadipic acid, deoxycytidine, glutamic acid, tryptophan, asparagine, alanine, cystine, hypoxanthine, uridine, aspartic acid, arginine, 2-hydroxybutyric acid, 2-hydroxyisovaleric acid, 3-hydroxyisovaleric acid, urea, 4-hydroxybenzoic acid, 4-aminobenzoic acid, ribonic acid, kynurenine, crystathionine, threonic acid, pyruvic acid, putrescine, ascorbic acid, riboflavin, serine, cysteine, orotic acid, and citric acid. According to an embodiment of the present invention, a differentiation state is evaluated based on abundances of two or more kinds of indicator substances.

公开(公告)号：US20180066221A1

公开(公告)日：2018-03-08

申请号：US15693763

申请日：2017-09-01

Applicant: TOKYO ELECTRON LIMITED

Inventor： Toshifumi KITAHARA ,  Kunitada HATABAYASHI ,  Kenichi KAGAWA ,  Yusuke YODA

IPC: C12M1/00 ,  C12M1/34 ,  C12M3/00

CPC classification number: C12M29/20 ,  C12M23/02 ,  C12M23/34 ,  C12M23/40 ,  C12M23/48 ,  C12M29/00 ,  C12M29/26 ,  C12M33/04 ,  C12M33/14 ,  C12M41/12 ,  C12M41/24 ,  C12M41/26 ,  C12M41/34 ,  C12M41/44 ,  C12M47/02

Abstract: A buffer tank for storing a culture medium for cell culture, includes: a tank main body including a cylindrical inner surface extending in a vertical direction, an inverted conical storage bottom surface formed below the cylindrical inner surface, and a storage space defined by the cylindrical inner surface and the inverted conical storage bottom surface and configured to store the culture medium an injection portion configured to inject the culture medium from the inverted conical storage bottom surface of the tank main body into the storage space; and a discharge portion configured to discharge the culture medium stored in the storage space, wherein the injection portion is configured to inject the culture medium toward a central axis line of the cylindrical inner surface of the tank main body and obliquely upward with respect to the central axis line.

公开(公告)号：US20170226558A1

公开(公告)日：2017-08-10

申请号：US15514610

申请日：2015-09-29

Applicant: TOKYO ELECTRON LIMITED

Inventor： Kunitada HATABAYASHI ,  Kentaro TOMITA ,  Shinichi GOMI ,  Tomoaki KURAKAZU ,  Yasuhiro OSHIMA ,  Kenichi KAGAWA ,  Shigenori OZAKI

IPC: C12Q1/02 ,  C12M1/00 ,  C12M1/26 ,  C12N5/00

Abstract: There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.