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    Method for producing a pyrimidine nucleoside compound and a new pyrimidine nucleoside compound
    1.
    发明申请
    Method for producing a pyrimidine nucleoside compound and a new pyrimidine nucleoside compound 审中-公开
    制备嘧啶核苷化合物和新的嘧啶核苷化合物的方法

    公开(公告)号：US20050074857A1

    公开(公告)日：2005-04-07

    申请号：US10891096

    申请日：2004-07-15

    申请人： Tadashi Araki ,  Hitoki Miyake ,  Toshihiro Oikawa

    发明人： Tadashi Araki ,  Hitoki Miyake ,  Toshihiro Oikawa

    IPC分类号： C12P19/40 ,  C07H17/02 ,  C07H19/067 ,  C07H19/073 ,  C07H19/04 ,  C12P19/30

    CPC分类号： C07H17/02 ,  C07H19/067 ,  C07H19/073

    摘要： A method for producing a pyrimidine nucleoside compound includes reacting a sugar phosphate and pyrimidine base derivative using an enzyme having cytosine nucleoside phosphorylase activity, the pyrimidine base derivative being represented by general formula (I): (wherein R1 represents an amino group that may be replaced with an acyl group having an alkyl group of 1 to 3 carbon atoms or an alkyl group of 1 to 3 carbon atoms, an alkyl group of 1 to 3 carbon atoms, or a thiol group; R2 represents an amino group, a thiol group, a hydroxyl group, or a hydrogen atom; R3 represents an alkyl group of 1 to 3 carbon atoms that may be replaced with a hydroxyl group, an amino group, or a hydrogen atom; R4 represents a hydroxyl group or a hydrogen atom; when R1 is an amino group, when R2 is a hydroxyl group, and when R4 is a hydrogen atom; R3 is neither an alkyl group of 1 to 3 carbon atoms nor a hydrogen atom).

    摘要翻译： 制备嘧啶核苷化合物的方法包括使用具有胞嘧啶核苷磷酸化酶活性的酶使糖磷酸盐和嘧啶碱衍生物反应，所述嘧啶碱衍生物由通式（I）表示：（其中R 1表示可被取代的氨基） 具有1〜3个碳原子的烷基或1〜3个碳原子的烷基，1〜3个碳原子的烷基或硫醇基的酰基; R2表示氨基，硫醇基， 羟基或氢原子; R 3表示可以被羟基，氨基或氢原子代替的1〜3个碳原子的烷基; R 4表示羟基或氢原子;当R 1 当R 2为羟基，当R 4为氢原子时，R 3为不含有1〜3个碳原子的烷基或氢原子）。

    Biocatalyst for production of d-lactic acid (as amended)
    2.
    发明申请
    Biocatalyst for production of d-lactic acid (as amended) 有权
    用于生产d-乳酸的生物催化剂（经修订）

    公开(公告)号：US20070065930A1

    公开(公告)日：2007-03-22

    申请号：US10573813

    申请日：2004-09-17

    申请人： Mitsufumi Wada ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    发明人： Mitsufumi Wada ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    IPC分类号： C12P7/56 ,  C12N1/21

    CPC分类号： C12P7/56

    摘要： A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

    摘要翻译： 提供高产率D-乳酸的制造方法，提供高纯度的高纯度D-副乳酸和副产物有机酸的生产方法。 在一个方面，培养其中丙酮酸甲酸裂合酶（pfl）的活性失活或降低，并且进一步增强大肠杆菌来源的NADH依赖性D-乳酸脱氢酶（ldhA）的活性的微生物，以有效地产生D- 乳酸。 关于增强ldhA活性的方法，通过在基因组上连接编码ldhA的基因与控制糖酵解途径参与蛋白质的表达的基因的启动子，核酸生物合成途径或氨基酸生物合成途径 与使用表达载体增强基因表达的方法相比，获得了合适的结果。 培养其中dld基因基本上失活或减少的微生物被培养以产生具有降低的丙酮酸浓度的高质量D-乳酸。

    Biocatalyst for production of D-lactic acid
    3.
    发明授权
    Biocatalyst for production of D-lactic acid 有权
    用于生产D-乳酸的生物催化剂

    公开(公告)号：US08669093B2

    公开(公告)日：2014-03-11

    申请号：US10573813

    申请日：2004-09-17

    申请人： Mitsufumi Wade ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    发明人： Mitsufumi Wade ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    IPC分类号： C12N1/21 ,  C12P7/56 ,  C12N1/26

    CPC分类号： C12P7/56

    摘要： A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

    摘要翻译： 提供高产率D-乳酸的制造方法，提供高纯度的高纯度D-副乳酸和副产物有机酸的生产方法。 在一个方面，培养其中丙酮酸甲酸裂合酶（pfl）的活性失活或降低，并且进一步增强大肠杆菌来源的NADH依赖性D-乳酸脱氢酶（ldhA）的活性的微生物，以有效地产生D- 乳酸。 关于增强ldhA活性的方法，通过在基因组上连接编码ldhA的基因与控制糖酵解途径参与蛋白质的表达的基因的启动子，核酸生物合成途径或氨基酸生物合成途径 与使用表达载体增强基因表达的方法相比，获得了合适的结果。 培养其中dld基因基本上失活或减少的微生物被培养以产生具有降低的丙酮酸浓度的高质量D-乳酸。

    Process For Producing Pentose-5-Phosphate Ester
    5.
    发明申请
    Process For Producing Pentose-5-Phosphate Ester 审中-公开
    生产戊糖-5-磷酸酯的方法

    公开(公告)号：US20070212763A1

    公开(公告)日：2007-09-13

    申请号：US10578912

    申请日：2004-11-09

    申请人： Keiichirou Kai ,  Hitoki Miyake ,  Toshihiro Oikawa

    发明人： Keiichirou Kai ,  Hitoki Miyake ,  Toshihiro Oikawa

    IPC分类号： C12P19/02 ,  C07H11/04

    CPC分类号： C12P19/44

    摘要： A pentose such as ribose, arabinose, 2-deoxyribose, 1-methoxy-2-deoxyribose or the like is reacted with a phosphoric acid donor such as a pyrophosphoric acid or an alkali metal salt thereof in the presence of an acid phosphatase. Thus, a pentose-5-phosphate ester can be produced, such as a ribose-5-phosphate ester, an arabinose-5-phosphate ester, a 2-deoxyribose-5-phosphate ester, a 1-methoxy-2-deoxyribose-5-phosphate ester or the like.

    摘要翻译： 在酸性磷酸酶的存在下，将诸如核糖，阿拉伯糖，2-脱氧核糖，1-甲氧基-2-脱氧核糖等的戊糖与磷酸供体如焦磷酸或其碱金属盐反应。 因此，可以制备戊糖-5-磷酸酯，例如核糖-5-磷酸酯，阿拉伯糖-5-磷酸酯，2-脱氧核糖-5-磷酸酯，1-甲氧基-2-脱氧核糖 - 5-磷酸酯等。