摘要:
The present invention provides a transformant having a gene expression inducing system having better responsiveness. The transformant of the invention has a galactose induction system and retains a coding region having a galactose induction system and encoding an assisting protein assisting transcription activation by a protein having the Gal4 activity under control of a constitutive promoter, and a coding region encoding an endogenous or exogenous desired protein under control of a galactose-inducible promoter.
摘要:
This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
摘要翻译:本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO:1(a)所示的核苷酸序列,并且在严格条件下与包含SEQ ID NO:1所示的全部或部分核苷酸序列或其互补链的探针杂交,并编码 具有D-乳酸脱氢酶活性的蛋白质(b)。
摘要:
The invention provides a promoter that can be used in the presence of an organic acid. The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts.
摘要:
This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
摘要翻译:本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO:1(a)所示的核苷酸序列,并且在严格条件下与包含SEQ ID NO:1所示的全部或部分核苷酸序列或其互补链的探针杂交,并编码 具有D-乳酸脱氢酶活性的蛋白质(b)。
摘要:
This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
摘要翻译:本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO:1(a)所示的核苷酸序列,并且在严格条件下与包含SEQ ID NO:1所示的全部或部分核苷酸序列或其互补链的探针杂交,并编码 具有D-乳酸脱氢酶活性的蛋白质(b)。
摘要:
Lactic acid with high optical purity that has not previously been achieved is produced.It has been found that the optical purity of lactic acid is reduced as the racemization reaction of lactic acid proceeds when lactic acid coexists with glycerol. By reducing the amount of glycerol prior to concentrating lactic acid by heating, the optical purity of lactic acid after concentration by heating can be maintained at a high level.
摘要:
Lactic acid with high optical purity that has not previously been achieved is produced. It has been found that the optical purity of lactic acid is reduced as the racemization reaction of lactic acid proceeds when lactic acid coexists with glycerol. By reducing the amount of glycerol prior to concentrating lactic acid by heating, the optical purity of lactic acid after concentration by heating can be maintained at a high level.
摘要:
The present teachings relate to an acid-resistant endoglucanase, which is a protein exhibiting excellent endoglucanase activity under acidic conditions. The present teachings provide a protein having the amino acid sequence set forth in SEQ ID NO: 2, a protein having an amino acid sequence with one or more amino acid modifications in the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity, or a protein having an amino acid sequence with at least 75% homology to the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity.
摘要翻译:本教导涉及耐酸内切葡聚糖酶,其是在酸性条件下表现出优异的内切葡聚糖酶活性的蛋白质。 本发明提供具有SEQ ID NO:2所示氨基酸序列的蛋白质,该蛋白质具有在SEQ ID NO:2所示的氨基酸序列中具有一个或多个氨基酸修饰的氨基酸序列的蛋白质,并具有内切葡聚糖酶 活性或具有与SEQ ID NO:2所示的氨基酸序列具有至少75%同源性且具有内切葡聚糖酶活性的氨基酸序列的蛋白质。
摘要:
The present teachings relate to an acid-resistant endoglucanase, which is a protein exhibiting excellent endoglucanase activity under acidic conditions. The present teachings provide a protein having the amino acid sequence set forth in SEQ ID NO: 2, a protein having an amino acid sequence with one or more amino acid modifications in the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity, or a protein having an amino acid sequence with at least 75% homology to the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity.
摘要翻译:本教导涉及耐酸内切葡聚糖酶,其是在酸性条件下表现出优异的内切葡聚糖酶活性的蛋白质。 本发明提供具有SEQ ID NO:2所示氨基酸序列的蛋白质,该蛋白质具有在SEQ ID NO:2所示的氨基酸序列中具有一个或多个氨基酸修饰的氨基酸序列的蛋白质,并具有内切葡聚糖酶 活性或具有与SEQ ID NO:2所示的氨基酸序列具有至少75%同源性且具有内切葡聚糖酶活性的氨基酸序列的蛋白质。
摘要:
The present invention provides a method for producing a useful substance efficiently from cellulose without using any cellulase preparation. According to the disclosures of the present specification, in the production of a useful substance from a cellulose-containing material, the cellulose-containing material is brought into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material, and a carbon source comprising the cellulose in the cellulose-containing material is simultaneously saccharified and fermented by a cellulase-producing microorganism in the presence of the ionic liquid.