利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

15 条记录 (耗时 0.028 秒)

    In-Vivo Non-Viral Gene Delivery of Human Vascular Endothelial Growth Factor Following Islet Transplantation
    6.
    发明申请
    In-Vivo Non-Viral Gene Delivery of Human Vascular Endothelial Growth Factor Following Islet Transplantation 审中-公开
    体外非病毒基因递送人类血管内皮生长因子胰岛移植后

    公开(公告)号：US20110182979A1

    公开(公告)日：2011-07-28

    申请号：US13014926

    申请日：2011-01-27

    申请人： Masayuki Shimoda ,  Shuyuan Chen ,  Hirofumi Noguchi ,  Shinichi Matsumoto ,  Paul A. Graybum

    发明人： Masayuki Shimoda ,  Shuyuan Chen ,  Hirofumi Noguchi ,  Shinichi Matsumoto ,  Paul A. Graybum

    IPC分类号： A61K9/127 ,  A61K48/00 ,  A61K38/18 ,  C12N5/00 ,  A61K35/39 ,  A61K31/713 ,  A61P3/10 ,  A61P1/16

    CPC分类号： A61K35/39 ,  A61K9/2077 ,  A61K38/1866 ,  A61K48/0075 ,  C12N15/87 ,  A61K2300/00

    摘要： An ultrasound-mediated gene transfer method named Ultrasound Targeted Microbubble Destruction (UTMD) for the delivery of human vascular endothelial growth factor (hVEGF) gene to transplanted islets and the surrounding tissue is described herein. The delivery of hVEGF promotes islet revascularization and survival. The inventors, first transplanted human islets were transplanted into diabetic nude mice liver followed by the induction of non-viral plasmid vectors encoding hVEGF or Green Fluorescent Protein (GFP) gene in the host liver by UTMD. Transplantation without gene delivery was also performed as a control. Blood glucose, serum human insulin, C-peptide levels and the revascularization in graft islets were evaluated. The findings of the method of the present invention indicated that hVEGF gene delivery to host liver using UTMD promoted islet revascularization after islet transplantation and improved the restoration of euglycemia.

    摘要翻译： 本文描述了称为超声靶向微泡破坏（UTMD）的超声介导的基因转移方法，用于将移植的胰岛和周围组织递送人血管内皮生长因子（hVEGF）基因。 hVEGF的输送促进胰岛血运重建和存活。 将本发明人首先将移植的人胰岛移植到糖尿病裸小鼠肝脏中，然后通过UTMD诱导宿主肝脏中编码hVEGF或绿色荧光蛋白（GFP）基因的非病毒质粒载体。 也进行无基因递送移植作为对照。 评估血糖，血清人胰岛素，C肽水平和移植胰岛血运重建。 本发明方法的发现表明，使用UTMD的hVEGF基因递送至宿主肝，促进了胰岛移植后胰岛血运重建，改善了正常血糖的恢复。

    Method of separating pancreatic islet
    7.
    发明授权
    Method of separating pancreatic islet 有权
    分离胰岛的方法

    公开(公告)号：US08288085B2

    公开(公告)日：2012-10-16

    申请号：US11793679

    申请日：2005-12-22

    申请人： Shinichi Matsumoto ,  Hiromi Wada ,  Hirofumi Noguchi

    发明人： Shinichi Matsumoto ,  Hiromi Wada ,  Hirofumi Noguchi

    IPC分类号： A01N1/00 ,  A01N1/02 ,  A01N63/02

    CPC分类号： C12N5/0677 ,  A01N1/0205 ,  A01N1/0226 ,  A61K35/12 ,  C12N2509/00

    摘要： The present invention provides a pancreatic islet isolation method comprising the steps of (1) injecting a protection solution containing a protease inhibitor into the pancreatic duct of an procured pancreas; (3) digesting the pancreas into which the protection solution has been injected; and (4) purifying the digested pancreatic tissue using a purification solution containing a density gradient reagent. The present invention also provides a protection solution for injection into the pancreatic duct, a pancreas preservation solution for the two-layer method, and an islet purification solution.

    摘要翻译： 本发明提供一种胰岛分离方法，其包括以下步骤：（1）将含有蛋白酶抑制剂的保护溶液注入胰腺胰管; （3）消化注入保护液的胰腺; 和（4）使用含有密度梯度试剂的纯化溶液纯化消化的胰腺组织。 本发明还提供了用于注射胰管的保护溶液，用于双层方法的胰脏保存溶液和胰岛净化溶液。