公开(公告)号：US08518651B2

公开(公告)日：2013-08-27

申请号：US12527191

申请日：2008-02-15

申请人： Taka-aki Sato ,  Atsuhiko Toyama ,  Takashi Shimada ,  Tetsuyoshi Sugita ,  Daisuke Aoki ,  Atsushi Suzuki ,  Nobuyuki Susumu ,  Hiroyuki Nomura

发明人： Taka-aki Sato ,  Atsuhiko Toyama ,  Takashi Shimada ,  Tetsuyoshi Sugita ,  Daisuke Aoki ,  Atsushi Suzuki ,  Nobuyuki Susumu ,  Hiroyuki Nomura

IPC分类号： G01N33/53

CPC分类号： G01N33/57449 ,  G01N2800/56

摘要： It is provided a method for identification of the morbidity of epithelial ovarian cancer based on a tissue-type in view of molecular typing which is different from a conventional histopathology, and a marker for identification of a tissue-type of epithelial ovarian cancer. A method for identification of the morbidity of epithelial ovarian cancer based on a tissue-type, comprising: subjecting a sample originated from an individual of interest to a treatment for detecting at least one selected from the group consisting of biological molecules specifically showing an upregulation in expression in a specific tissue-type of epithelial ovarian cancer, and/or at least one selected from the group consisting of biological molecules specifically showing a downregulation in expression in a specific tissue-type of epithelial ovarian cancer, and identifying whether or not the significant detection of the protein is achieved, thereby identifying the tissue-type.

摘要翻译： 提供了一种鉴定与常规组织病理学不同的分子分型的基于组织型的上皮性卵巢癌的发病率的方法和用于鉴定组织型上皮性卵巢癌的标志物。 一种用于鉴定基于组织型的上皮性卵巢癌的发病率的方法，其包括：对来自感兴趣个体的样品进行处理，以检测选自特异性显示上调的生物分子中的至少一种 在特定组织型上皮性卵巢癌中的表达，和/或选自特异性显示特定组织型上皮性卵巢癌中的下调的生物分子中的至少一种，以及鉴定是否显着 实现蛋白质的检测，从而鉴定组织型。

公开(公告)号：US20100086938A1

公开(公告)日：2010-04-08

申请号：US12516790

申请日：2007-10-02

申请人： Takashi Shimada ,  Atsuhiko Toyama ,  Taka-aki Sato

发明人： Takashi Shimada ,  Atsuhiko Toyama ,  Taka-aki Sato

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/552 ,  G01N33/544

CPC分类号： G01N33/6848

摘要： It is intended to provide an analysis method for a biological sample with clinical significance, which is a system being simple to such an extent that it can be applied to a clinical field, and capable of capping a molecule ionization suppression in mass spectrometry. Mass spectrometry of a biological sample using immunoprecipitation, comprising the steps of: preparing a sample containing an objective biological molecule; concentrating the biological molecule in the sample by immunoprecipitation using an antibody-bound carrier or a carrier to which a molecule capable of specifically binding to an antibody is bound; washing an immunoprecipitate of the biological molecule by using an aqueous solution containing a charge neutralizing agent as a washing liquid; and detecting the biological molecule by subjecting the washed immunoprecipitate to mass spectrometry.

摘要翻译： 旨在为具有临床意义的生物样品提供分析方法，该方法简单到能够应用于临床领域并且能够在质谱法中限制分子离子化抑制的系统。 使用免疫沉淀的生物样品的质谱法，包括以下步骤：制备含有客观生物分子的样品; 通过使用抗体结合的载体或能够与抗体特异性结合的分子结合的载体通过免疫沉淀将生物分子浓缩在样品中; 通过使用含有电荷中和剂的水溶液作为洗涤液来洗涤生物分子的免疫沉淀物; 并通过对洗涤的免疫沉淀物进行质谱检测来检测生物分子。

公开(公告)号：US08283625B2

公开(公告)日：2012-10-09

申请号：US12443461

申请日：2007-09-21

申请人： Taka-aki Sato ,  Yutaka Aoki ,  Takashi Shimada ,  Atsuhiko Toyama

发明人： Taka-aki Sato ,  Yutaka Aoki ,  Takashi Shimada ,  Atsuhiko Toyama

IPC分类号： B01D59/44

CPC分类号： H01J49/164 ,  G01N1/2806 ,  G01N1/2813 ,  H01J49/0004 ,  H01J49/0418

摘要： It is intended to provide a method of preparing sample for matrix-assisted laser desorption/ionization mass spectrometry using a matrix capable of generating preferred crystals that cause effective ionization of a molecule to be measured. A method of preparing a sample for matrix-assisted laser desorption/ionization mass spectrometry comprising the steps of: preparing a solution of 2,5-dihydroxybenzoic acid of 40 mg/mL to saturated concentration as a matrix solution; and dispensing the matrix solution to a sample to be analyzed by using an inkjet mechanism, to crystallize the 2,5-dihydroxybenzoic acid.

摘要翻译： 旨在提供一种使用能够产生优选的晶体的基质辅助激光解吸/电离质谱法的样品的制备方法，所述晶体能够产生有效电离被测分子。 一种制备用于基体辅助激光解吸/电离质谱的样品的方法，包括以下步骤：以饱和浓度制备40mg / mL的2,5-二羟基苯甲酸溶液作为基质溶液; 并通过使用喷墨机构将基质溶液分配到要分析的样品上，使2,5-二羟基苯甲酸结晶。

公开(公告)号：US20100075354A1

公开(公告)日：2010-03-25

申请号：US12527191

申请日：2008-02-15

申请人： Taka-aki Sato ,  Atsuhiko Toyama ,  Takashi Shimada ,  Tetsuyoshi Sugita ,  Daisuke Aoki ,  Atsushi Suzuki ,  Nobuyuki Susumu ,  Hiroyuki Nomura

发明人： Taka-aki Sato ,  Atsuhiko Toyama ,  Takashi Shimada ,  Tetsuyoshi Sugita ,  Daisuke Aoki ,  Atsushi Suzuki ,  Nobuyuki Susumu ,  Hiroyuki Nomura

IPC分类号： C12Q1/48 ,  C12Q1/34 ,  C12Q1/02 ,  C12N9/10 ,  C12N9/14 ,  C07K14/00

CPC分类号： G01N33/57449 ,  G01N2800/56

摘要： It is provided a method for identification of the morbidity of epithelial ovarian cancer based on a tissue-type in view of molecular typing which is different from a conventional histopathology, and a marker for identification of a tissue-type of epithelial ovarian cancer. A method for identification of the morbidity of epithelial ovarian cancer based on a tissue-type, comprising: subjecting a sample originated from an individual of interest to a treatment for detecting at least one selected from the group consisting of biological molecules specifically showing an upregulation in expression in a specific tissue-type of epithelial ovarian cancer, and/or at least one selected from the group consisting of biological molecules specifically showing a downregulation in expression in a specific tissue-type of epithelial ovarian cancer, and identifying whether or not the significant detection of the protein is achieved, thereby identifying the tissue-type.

摘要翻译： 提供了一种鉴定与常规组织病理学不同的分子分型的基于组织型的上皮性卵巢癌的发病率的方法和用于鉴定组织型上皮性卵巢癌的标志物。 一种用于鉴定基于组织型的上皮性卵巢癌的发病率的方法，其包括：对来自感兴趣个体的样品进行处理，以检测选自特异性显示上调的生物分子中的至少一种 在特定组织型上皮性卵巢癌中的表达，和/或选自特异性显示特定组织型上皮性卵巢癌中的下调的生物分子中的至少一种，以及鉴定是否显着 实现蛋白质的检测，从而鉴定组织型。

公开(公告)号：US20100075372A1

公开(公告)日：2010-03-25

申请号：US12443437

申请日：2007-09-21

申请人： Taka-aki Sato ,  Yutaka Aoki ,  Takashi Shimada ,  Atsuhiko Toyama

发明人： Taka-aki Sato ,  Yutaka Aoki ,  Takashi Shimada ,  Atsuhiko Toyama

IPC分类号： G01N1/28

CPC分类号： G01N1/30 ,  G01N1/36 ,  H01J49/0004

摘要： The present invention provides a method for deparaffinization useful for mass spectrometric imaging from a paraffin-embedded specimen. A method for deparaffinization of paraffin-embedded specimen comprising the steps of: exposing a biological sample by subjecting a specimen in which the biological sample is embedded in paraffin, to an organic solvent that is compatible with the paraffin under heating condition, to make the paraffin be melted and dissolved in the organic solvent; and removing the paraffin from the specimen by separating the organic solvent dissolving the paraffin, from the exposed biological sample.

摘要翻译： 本发明提供了可用于石蜡包埋样品的质谱成像的脱蜡方法。 一种石蜡包埋试样的脱蜡方法，其特征在于，包括以下步骤：使生物样品在石蜡中包埋的样品在加热条件下与石蜡相容的有机溶剂进行曝光，使石蜡 熔化并溶解在有机溶剂中; 通过从暴露的生物样品中分离溶解石蜡的有机溶剂，从样品中除去石蜡。

公开(公告)号：US20100038529A1

公开(公告)日：2010-02-18

申请号：US12443461

申请日：2007-09-21

申请人： Taka-aki Sato ,  Yutaka Aoki ,  Takashi Shimada ,  Atsuhiko Toyama

发明人： Taka-aki Sato ,  Yutaka Aoki ,  Takashi Shimada ,  Atsuhiko Toyama

IPC分类号： H01J49/02 ,  B01D59/44

CPC分类号： H01J49/164 ,  G01N1/2806 ,  G01N1/2813 ,  H01J49/0004 ,  H01J49/0418

摘要： It is intended to provide a method of preparing sample for matrix-assisted laser desorption/ionization mass spectrometry using a matrix capable of generating preferred crystals that cause effective ionization of a molecule to be measured. A method of preparing a sample for matrix-assisted laser desorption/ionization mass spectrometry comprising the steps of: preparing a solution of 2,5-dihydroxybenzoic acid of 40 mg/mL to saturated concentration as a matrix solution; and dispensing the matrix solution to a sample to be analyzed by using an inkjet mechanism, to crystallize the 2,5-dihydroxybenzoic acid.

摘要翻译： 旨在提供一种使用能够产生优选的晶体的基质辅助激光解吸/电离质谱法的样品的制备方法，所述晶体能够引起待测分子的有效电离。 一种制备用于基体辅助激光解吸/电离质谱的样品的方法，包括以下步骤：以饱和浓度制备40mg / mL的2,5-二羟基苯甲酸溶液作为基质溶液; 并通过使用喷墨机构将基质溶液分配到要分析的样品上，使2,5-二羟基苯甲酸结晶。