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公开(公告)号：US07422888B2
公开(公告)日：2008-09-09
申请号：US10380430
申请日：2001-09-13
申请人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kiyozo Asada , Ikunoshin Kato
CPC分类号： C12N9/22
摘要： Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译： 具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。
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公开(公告)号：US20090098600A1
公开(公告)日：2009-04-16
申请号：US12194364
申请日：2008-08-19
申请人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
CPC分类号： C12N9/22
摘要： Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译： 具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。
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公开(公告)号：US08008055B2
公开(公告)日：2011-08-30
申请号：US12194364
申请日：2008-08-19
申请人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
CPC分类号： C12N9/22
摘要： Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译： 具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。
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公开(公告)号：US06921656B1
公开(公告)日：2005-07-26
申请号：US10048621
申请日：2000-07-26
申请人： Nobuto Koyama , Toshitake Okui , Hikaru Takakura , Kiyozo Asada , Ikunoshin Kato
发明人： Nobuto Koyama , Toshitake Okui , Hikaru Takakura , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12N1/15 , C12N1/19 , C12N1/21 , C12N9/26 , C12N9/28 , C12N9/34 , C12N15/56 , C12P19/04 , C12P19/14 , C12P19/20 , C07H21/04 , C12N15/52
CPC分类号： C12N9/2428 , C12N9/2414 , C12N9/2417 , C12P19/04 , C12P19/14 , C12P19/20
摘要： Polypeptides having the amino acid sequence represented by SEQ ID NO:6 in Sequence Listing or an amino acid sequence derived from the above sequence by at least one of deletion, addition, insertion or substitution of one or more amino acids and showing a thermophilic glucoamylase activity.
摘要翻译： 或具有由序列表中SEQ ID NO：6表示的氨基酸序列的多肽或由上述序列衍生的氨基酸序列，通过至少一种缺失，添加，插入或取代一个或多个氨基酸并显示出嗜热葡糖淀粉酶活性 。
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5.发明授权Enhancement of retroviral gene transduction employing polypeptides comprising the fibronectin heparin II binding domain 失效使用包含纤连蛋白肝素II结合结构域的多肽增强逆转录病毒基因转导公开(公告)号：US07790849B2
公开(公告)日：2010-09-07
申请号：US12241581
申请日：2008-09-30
申请人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
CPC分类号： C12N15/86 , A61K48/00 , C07K14/505 , C07K14/78 , C07K2319/00 , C12N9/1088 , C12N2740/13043 , C12N2740/13045
摘要： An isolated polypeptide includes the amino acid sequence shown in SEQ. ID No. 13 and an isolated nucleic acid encoding the polypeptide, such as an isolated nucleic acid including the nucleic acid sequence shown in SEQ. ID No. 17. The isolated polypeptide includes two heparin binding polypeptides connected in tandem and the isolated nucleic acid encodes these. The polypeptide and nucleic acid sequences can improve retroviral vector mediated gene transfer efficiency into target cells.
摘要翻译： 分离的多肽包括SEQ ID NO：1所示的氨基酸序列。 ID号13和编码该多肽的分离的核酸，例如分离的核酸，包括SEQ ID NO：1所示的核酸序列。 分离的多肽包括串联连接的两个肝素结合多肽，分离的核酸编码这些。 多肽和核酸序列可以改进逆转录病毒载体介导的靶细胞的基因转移效率。
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公开(公告)号：US20060030046A1
公开(公告)日：2006-02-09
申请号：US11181091
申请日：2005-07-14
申请人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
IPC分类号： C12N15/867 , C07K14/50
CPC分类号： C12N15/86 , A61K48/00 , C07K14/505 , C07K14/78 , C07K2319/00 , C12N9/1088 , C12N2740/13043 , C12N2740/13045
摘要： A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17.
摘要翻译： SEQ ID NO： SEQ ID NO：13，SEQ ID NO： SEQ ID No 30或其功能等同物和SEQ ID NO： ID号17。
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公开(公告)号：US06949623B2
公开(公告)日：2005-09-27
申请号：US09775964
申请日：2001-02-02
申请人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
IPC分类号： A61K48/00 , C07K14/505 , C07K14/78 , C12N9/10 , C12N15/12 , C12N15/867 , C07K1/00
CPC分类号： C12N15/86 , A61K48/00 , C07K14/505 , C07K14/78 , C07K2319/00 , C12N9/1088 , C12N2740/13043 , C12N2740/13045
摘要： The present invention provides a method for increasing the efficiency of gene transfer into target cells with a retrovirus. The transduction is affected by infecting target cells with a retrovirus in the presence of a mixture of a functional material having a retrovirus binding domain, and a second functional material having target cell binding domain. The target cells may be selected from the group of unintentional hematopoietic progenitor cells and erythrocyte precursor, specifically pluripotent stem cells or embryopalstic stem cells.
摘要翻译： 本发明提供了用逆转录病毒提高基因转移到靶细胞的效率的方法。 在具有逆转录病毒结合域的功能材料和具有靶细胞结合结构域的第二功能材料的混合物存在下，用逆转录病毒感染靶细胞影响转导。 靶细胞可以选自无意造血祖细胞和红细胞前体，特别是多能干细胞或胚胎干细胞。
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8.发明授权Methods and kits for improving retroviral-mediated gene transfer utilizing molecules, or mixture thereof, containing retroviral binding domains and target cell binding domains 失效使用含有逆转录病毒结合结构域和靶细胞结合结构域的分子或其混合物来改进逆转录病毒介导的基因转移的方法和试剂盒公开(公告)号：US06426042B1
公开(公告)日：2002-07-30
申请号：US09366009
申请日：1999-08-02
申请人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
IPC分类号： G01N3122
CPC分类号： C12N15/86 , A61K48/00 , C07K14/505 , C07K14/78 , C07K2319/00 , C12N9/1088 , C12N2740/13043 , C12N2740/13045
摘要： The present invention provides a kit to carry out retrovirus-mediated gene transfer into target cells. The kit contains a functional material bearing a retrovirus binding domain, another functional material bearing a target cell binding domain, an artificial substrate for incubating the retrovirus contacted with the target cells, and a target cell growth factor for pre-stimulating target cells to spur them along the cell cycle. The kit of the invention may further comprise a recombinant retroviral vector, necessary buffers, and the like.
摘要翻译： 本发明提供了一种用于将逆转录病毒介导的基因转移进入靶细胞的试剂盒。 该试剂盒包含具有逆转录病毒结合结构域的功能材料，具有靶细胞结合结构域的另一功能材料，用于孵育与靶细胞接触的逆转录病毒的人造底物，以及用于预刺激靶细胞刺激它们的靶细胞生长因子 沿细胞周期。 本发明的试剂盒还可以包含重组逆转录病毒载体，必需的缓冲液等。
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公开(公告)号：US20090209730A1
公开(公告)日：2009-08-20
申请号：US12241581
申请日：2008-09-30
申请人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
CPC分类号： C12N15/86 , A61K48/00 , C07K14/505 , C07K14/78 , C07K2319/00 , C12N9/1088 , C12N2740/13043 , C12N2740/13045
摘要： A polypeptide represented by SEQ. ID No. 13 and a gene encoding the polypeptide.A gene is also represented by SEQ. ID No. 17, or a gene hybridizable thereto under stringent conditions and encoding a polypeptide which improves the efficiency of gene transfer into target cells with a retrovirus.
摘要翻译： SEQ ID NO： ID号13和编码该多肽的基因。 基因也由SEQ代表。 ID号17，或其在严格条件下可与其杂交的基因，并编码多肽，其使用逆转录病毒提高基因转移到靶细胞的效率。
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公开(公告)号：US07446170B2
公开(公告)日：2008-11-04
申请号：US11181091
申请日：2005-07-14
申请人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
IPC分类号： C07K1/00
CPC分类号： C12N15/86 , A61K48/00 , C07K14/505 , C07K14/78 , C07K2319/00 , C12N9/1088 , C12N2740/13043 , C12N2740/13045
摘要： A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17.
摘要翻译： SEQ ID NO： SEQ ID NO：13，SEQ ID NO： SEQ ID No 30或其功能等同物和SEQ ID NO： ID号17。
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