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    Gene transfer method
    1.
    发明授权
    Gene transfer method 失效
    基因转移法

    公开(公告)号：US06995010B1

    公开(公告)日：2006-02-07

    申请号：US10111708

    申请日：2000-10-23

    申请人： Takashi Ueno ,  Hajime Matsumura ,  Keiji Tanaka ,  Tomoko Iwasaki ,  Mitsuhiro Ueno ,  Kei Fujinaga ,  Kiyozo Asada ,  Ikunoshin Kato

    发明人： Takashi Ueno ,  Hajime Matsumura ,  Keiji Tanaka ,  Tomoko Iwasaki ,  Mitsuhiro Ueno ,  Kei Fujinaga ,  Kiyozo Asada ,  Ikunoshin Kato

    IPC分类号： C12N5/16 ,  C12N15/861 ,  C12N15/864 ,  A61K48/00

    CPC分类号： C12N15/86 ,  C12N2710/10343 ,  C12N2800/30

    摘要： A method of transferring a foreign gene into cells, characterized by involving: the step of transferring into the cells with the use of an adenovirus vector, a first nucleic acid, which has a sequence provided with adeno-associated virus-origin ITRs in both sides of the target foreign gene to be transferred, and a second nucleic acid, which has an adeno-associated virus-origin rep gene and a promoter for expressing this gene and carries a stuffer sequence inserted thereinto sandwiched in two recombinase recognition sequences and located between the rep gene and the promoter; and the step of expressing the Rep protein under the action of recombinase in the cells obtained in the above step to thereby integrate the target foreign gene into the chromosomal DNA.

    摘要翻译： 将外源基因转移到细胞中的方法，其特征在于包括：使用腺病毒载体转移到细胞中的步骤，第一核酸，其具有在两侧具有腺相关病毒起源ITR的序列 的待转移的目标外源基因的第二核酸和具有腺相关病毒起源rep基因的第二核酸和用于表达该基因的启动子，并携带插入其中的填充序列夹在两个重组酶识别序列中并位于 rep基因和启动子; 以及在上述步骤中获得的细胞中在重组酶的作用下表达Rep蛋白的步骤，从而将靶外源基因整合到染色体DNA中。

    Methods for retroviral mediated gene transfer employing molecules, or mixtures thereof, containing retroviral binding domains and target cell binding domains
    9.
    发明授权
    Methods for retroviral mediated gene transfer employing molecules, or mixtures thereof, containing retroviral binding domains and target cell binding domains 失效
    使用含有逆转录病毒结合结构域和靶细胞结合结构域的分子或其混合物进行逆转录病毒介导的基因转移的方法

    公开(公告)号：US06472204B1

    公开(公告)日：2002-10-29

    申请号：US08809156

    申请日：1997-03-07

    申请人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimakazu Hasino ,  Ikunoshin Kato

    发明人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimakazu Hasino ,  Ikunoshin Kato

    IPC分类号： C12N1500

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/505 ,  C07K14/78 ,  C07K2319/00 ,  C12N9/1088 ,  C12N2740/13043 ,  C12N2740/13045

    摘要： A method is disclosed for increasing the efficiency of retroviral mediated gene transfer into viable target cells, which comprises transducing the target cells by infecting the target cells with a replication defective recombinant retrovirus that infects the target cells in an aqueous medium in the presence of (a) a mixture of an effective amount of a first functional material having a retrovirus binding domain that binds said retrovirus, and an effective amount of a second functional material having a target cell binding domain that binds said target cell, or (b) an effective amount of a bifunctional material having both a retroviral binding domain which does not contain the heparin binding domain derived from human fibronectin, and a target cell binding domain, wherein the bifunctional material has a retrovirus binding domain that binds to said retrovirus and a target cell binding domain that binds to the target cell.

    摘要翻译： 公开了一种用于增加逆转录病毒介导的基因转移到活的靶细胞中的效率的方法，该方法包括通过用在（a）的存在下在水性介质中感染靶细胞的复制缺陷型重组逆转录病毒感染靶细胞来转导靶细胞 ）有效量的具有结合所述逆转录病毒的逆转录病毒结合结构域的第一功能材料和有效量的具有结合所述靶细胞的靶细胞结合结构域的第二功能材料的混合物，或（b）有效量 具有不含源自人纤连蛋白的肝素结合结构域的逆转录病毒结合结构域的双功能材料和靶细胞结合结构域，其中所述双功能材料具有结合所述逆转录病毒的逆转录病毒结合结构域和靶细胞结合结构域 其结合靶细胞。

    Method of searching for functional nucleotide molecule
    10.
    发明申请
    Method of searching for functional nucleotide molecule 审中-公开
    搜索功能核苷酸分子的方法

    公开(公告)号：US20070077563A1

    公开(公告)日：2007-04-05

    申请号：US10569814

    申请日：2004-08-25

    申请人： Takashi Ueno ,  Masashige Tanabe ,  Risa Sumioka ,  Eiji Kobayashi ,  Nobuto Koyama ,  Hiroaki Sagawa ,  Junichi Mineno ,  Ikunoshin Kato

    发明人： Takashi Ueno ,  Masashige Tanabe ,  Risa Sumioka ,  Eiji Kobayashi ,  Nobuto Koyama ,  Hiroaki Sagawa ,  Junichi Mineno ,  Ikunoshin Kato

    IPC分类号： C12Q1/68 ,  C07H21/02

    CPC分类号： C12N15/63

    摘要： A universal method of searching for a nucleic acid capable of effectively inhibiting gene expression; a nucleic acid construct to be used in this method; a vector; and a kit for the above method. This method is characterized in that a nucleic acid construct, which is a nucleic acid construct having a promoter sequence, at least two gene sequences and a poly A signal sequence and in which the above-described at least two gene sequences are transcribed as a single molecule RNA and at least one gene sequence is in the translatable state while at least one gene sequence is encoded in the substantially untranslatable state, is constructed.

    摘要翻译： 搜索能够有效抑制基因表达的核酸的通用方法; 在该方法中使用的核酸构建体; 一个矢量 和上述方法的试剂盒。 该方法的特征在于，具有启动子序列，至少两个基因序列和多聚A信号序列的核酸构建体的核酸构建体，其中上述至少两个基因序列被转录为单个 分子RNA和至少一个基因序列处于可翻译状态，而至少一个基因序列被编码为基本上不可翻译的状态。