公开(公告)号：US07092151B2

公开(公告)日：2006-08-15

申请号：US10889063

申请日：2004-07-13

申请人： Tatsuro Otaki ,  Yoshitaro Nakano

发明人： Tatsuro Otaki ,  Yoshitaro Nakano

IPC分类号： G02B21/00

CPC分类号： G02B21/26 ,  G01N35/109 ,  G01N2035/1062 ,  G02B21/0088

摘要： A well plate holding a specimen to be observed is placed on a fixed stage, and the specimen is observed through an objective lens disposed below the well plate. A well set on the optical axis is illuminated through transmitted illumination provided by a transmitted illumination device. The transmitted illumination device includes a plurality of LEDs disposed to form a ring shape, and a through hole is formed further inward relative to the LEDs. When injecting a reagent into the well being observed, the reagent is drawn by using a head at a pipette device, and then the head is moved to a point above the well being observed through the hole formed at the transmitted illumination device.

摘要翻译： 将待观察的试样的孔板放置在固定台上，并通过设置在孔板下方的物镜观察样品。 通过由透射照明装置提供的透射照明照射光轴上的良好设置。 透射照明装置包括多个设置成环形的LED，并且相对于LED进一步向内形成通孔。 当将试剂注入观察到的井中时，通过在移液管装置上使用头部来吸取试剂，然后通过形成在透射照明装置上形成的孔将头移动到通过孔观察的点上。

公开(公告)号：US20050012990A1

公开(公告)日：2005-01-20

申请号：US10889063

申请日：2004-07-13

申请人： Tatsuro Otaki ,  Yoshitaro Nakano

发明人： Tatsuro Otaki ,  Yoshitaro Nakano

IPC分类号： G01N35/10 ,  G02B21/00 ,  G02B21/26 ,  G02B21/32

CPC分类号： G02B21/26 ,  G01N35/109 ,  G01N2035/1062 ,  G02B21/0088

摘要： A well plate holding a specimen to be observed is placed on a fixed stage, and the specimen is observed through an objective lens disposed below the well plate. A well set on the optical axis is illuminated through transmitted illumination provided by a transmitted illumination device. The transmitted illumination device includes a plurality of LEDs disposed to form a ring shape, and a through hole is formed further inward relative to the LEDs. When injecting a reagent into the well being observed, the reagent is drawn by using a head at a pipette device, and then the head is moved to a point above the well being observed through the hole formed at the transmitted illumination device.

摘要翻译： 将待观察的试样的孔板放置在固定台上，并通过设置在孔板下方的物镜观察样品。 通过由透射照明装置提供的透射照明照射光轴上的良好设置。 透射照明装置包括多个设置成环形的LED，并且相对于LED进一步向内形成通孔。 当将试剂注入观察到的井中时，通过在移液管装置上使用头部来吸取试剂，然后通过形成在透射照明装置上形成的孔将头移动到通过孔观察的点上。

公开(公告)号：US20060210962A1

公开(公告)日：2006-09-21

申请号：US11390419

申请日：2006-03-28

申请人： Yuji Imaizumi ,  Yoshitaro Nakano ,  Ichiro Sase

发明人： Yuji Imaizumi ,  Yoshitaro Nakano ,  Ichiro Sase

IPC分类号： C12Q1/00 ,  C12M1/34

CPC分类号： G01N21/253 ,  C12Q1/04

摘要： The cell observation device and method aims to easily study the correlation between initial states of cells and their reactions without using a synchronous culture method. For this purpose, the cell observation device includes an image acquisition unit acquiring an image of a specimen including a plurality of cells; a stimulating unit applying a predetermined stimulation to the plural cells; a determining unit determining initial states of the plural cells from the image which is acquired by the image acquisition unit before the stimulating unit applies the stimulation; a finding unit finding reactions of the plural cells to the stimulation, from the image acquired by the image acquisition unit after the stimulating unit applies the stimulation; and a generating unit generating information on correlation between the initial states and the reactions of the plural cells.

摘要翻译： 细胞观察装置和方法旨在容易地研究细胞初始状态与其反应之间的相关性，而不使用同步培养方法。 为此，细胞观察装置包括获取包含多个细胞的标本的图像的图像获取单元; 刺激单元，对所述多个细胞施加预定的刺激; 确定单元，在所述刺激单元施加所述刺激之前，从所述图像获取单元获取的图像中确定所述多个单元的初始状态; 发现单元从刺激单元施加刺激之后由图像获取单元获取的图像中发现多个单元对刺激的反应; 以及生成单元，生成关于初始状态与多个单元的反应之间的相关性的信息。

公开(公告)号：US07415144B2

公开(公告)日：2008-08-19

申请号：US11390419

申请日：2006-03-28

申请人： Yuji Imaizumi ,  Yoshitaro Nakano ,  Ichiro Sase

发明人： Yuji Imaizumi ,  Yoshitaro Nakano ,  Ichiro Sase

IPC分类号： G06K9/00

CPC分类号： G01N21/253 ,  C12Q1/04

摘要： The cell observation device and method aims to easily study the correlation between initial states of cells and their reactions without using a synchronous culture method. For this purpose, the cell observation device includes an image acquisition unit acquiring an image of a specimen including a plurality of cells; a stimulating unit applying a predetermined stimulation to the plural cells; a determining unit determining initial states of the plural cells from the image which is acquired by the image acquisition unit before the stimulating unit applies the stimulation; a finding unit finding reactions of the plural cells to the stimulation, from the image acquired by the image acquisition unit after the stimulating unit applies the stimulation; and a generating unit generating information on correlation between the initial states and the reactions of the plural cells.

摘要翻译： 细胞观察装置和方法旨在容易地研究细胞初始状态与其反应之间的相关性，而不使用同步培养方法。 为此，细胞观察装置包括获取包含多个细胞的标本的图像的图像获取单元; 刺激单元，对所述多个细胞施加预定的刺激; 确定单元，在所述刺激单元施加所述刺激之前，从所述图像获取单元获取的图像中确定所述多个单元的初始状态; 发现单元从刺激单元施加刺激之后由图像获取单元获取的图像中发现多个单元对刺激的反应; 以及生成单元，生成关于初始状态与多个单元的反应之间的相关性的信息。

公开(公告)号：US08588502B2

公开(公告)日：2013-11-19

申请号：US12979858

申请日：2010-12-28

申请人： Yoshitaro Nakano ,  Yousuke Takahama ,  Takeshi Nitta

发明人： Yoshitaro Nakano ,  Yousuke Takahama ,  Takeshi Nitta

IPC分类号： G06K9/00 ,  G06K9/46

CPC分类号： G01N15/1463 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6458 ,  G01N2015/1443

摘要： Statistical data of a fluorescence population is obtained easily with high accuracy. To achieve this, an evaluation apparatus of fluorescence population of the present application includes an input unit inputting a fluorescence observation image (If) of a fluorescence population and a transillumination observation image (It) having a same field of view as the fluorescence observation image, a first detecting unit (Mt) detecting, as a first area, an area in which a cellular image exists on the transillumination observation image being input, a setting unit (If′) setting, as a reference area, an area corresponding to the first area on the fluorescence observation image being input, and an obtaining unit obtaining fluorescence intensity data of a fluorescence image from the reference area in the fluorescence observation image.

摘要翻译： 以高精度容易地获得荧光群体的统计学数据。 为了实现这一点，本申请的荧光群的评价装置包括输入荧光群的荧光观察图像（If）的输入单元和与荧光观察图像具有相同视场的透照观察图像（It） 第一检测单元（Mt）作为第一区域检测正在输入的透照观察图像上存在蜂窝图像的区域，设置单元（If'）设置作为参考区域，与第一区域相对应的区域 输入荧光观察图像的面积，以及从荧光观察图像中的参考区域获取荧光图像的荧光强度数据的获取单元。

公开(公告)号：US07371565B2

公开(公告)日：2008-05-13

申请号：US10671721

申请日：2003-09-29

申请人： Yuji Imaizumi ,  Takayuki Suga ,  Yoshitaro Nakano

发明人： Yuji Imaizumi ,  Takayuki Suga ,  Yoshitaro Nakano

IPC分类号： C12M1/34

CPC分类号： G01N21/6458 ,  G01N2015/0065 ,  G06K9/00127 ,  G06T7/0012 ,  G06T2207/30024

摘要： An intracellular-reaction measuring apparatus for measuring intracellular reactions by the use of a specimen in which a plurality of cell colonies are contained in a non-contact state, the apparatus includes a device that specifies the intensity of first light emitted from the specimen in accordance with the presence of a stated protein is detected to specify, of the plurality of cell colonies, a noted colony containing cells where the stated protein is present, and a device in which the intensity of second light emitted from the specimen in accordance with the intracellular reactions is detected to select, of the detected intensity of the second light, the intensity of the second light emitted from the noted colony.

摘要翻译： 一种细胞内反应测定装置，其通过使用非接触状态下容纳多个细胞集落的样本来测定细胞内反应，所述装置包括：根据所述试样确定从所述试样发出的第一光的强度的装置 检测到所述蛋白质的存在以指定多个细胞集落中存在所述蛋白质的所述含有细胞的所述集落，以及其中根据细胞内从样品发射的第二光的强度的装置 检测到反应，以检测第二光的检测强度，从所述菌落发射的第二光的强度。

公开(公告)号：US6025917A

公开(公告)日：2000-02-15

申请号：US68087

申请日：1998-05-01

申请人： Shuji Toyonaga ,  Masahisa Shiroshita ,  Takayuki Suga ,  Yoshitaro Nakano

发明人： Shuji Toyonaga ,  Masahisa Shiroshita ,  Takayuki Suga ,  Yoshitaro Nakano

IPC分类号： G01J3/44 ,  G01J3/447 ,  G01J4/00 ,  G01N21/64 ,  G01N21/65 ,  G02B21/06 ,  G02F1/01

CPC分类号： G01N21/6445 ,  G01J3/4406 ,  G01J3/447 ,  G01J4/00 ,  G01N21/65

摘要： Provided is a polarization characteristic measuring method and apparatus for accurately measuring a polarization characteristic of fluorescence or Raman-scattered light emitted when a sample is exposed to light. The sample is exposed to excitation light radiated from a pulsed excitation light source and converted to p-polarized light by polarizer and half-wave plate, and photodetectors measure an intensity I.sub.pp of a p-polarized component and an intensity I.sub.ps of an s-polarized component of fluorescence emitted from the sample under irradiation with the excitation light. In similar fashion, the sample is exposed to the excitation light of s-polarized light and the detectors measure an intensity I.sub.sp of a p-polarized component and an intensity I.sub.ss of an s-polarized component of fluorescence emitted from the sample under irradiation. From these measured values, G factor is calculated according to the following equation:G=[(I.sub.pp .multidot.I.sub.sp)/(I.sub.ps .multidot.I.sub.ss)].sup.1/2and polarization response correction is effected based on this G factor to obtain the polarization characteristic of fluorescence.

摘要翻译： PCT No.PCT / JP97 / 03391 Sec。 371日期：1998年5月1日 102（e）日期1998年5月1日PCT 1997年9月24日PCT公布。 第WO98 / 13676号公报 日期1998年4月2日提供了一种用于精确测量样品曝光时发出的荧光或拉曼散射光的偏振特性的偏振特性测量方法和装置。 将样品暴露于从脉冲激发光源辐射的激发光，并通过偏振器和半波片转换成p偏振光，光电检测器测量p偏振分量的强度Ipp和s偏振的强度Ips 在用激发光照射下从样品发射的荧光的分量。 以类似的方式，将样品暴露于s偏振光的激发光，并且检测器测量p偏振分量的强度Isp和照射下从样品发射的荧光的s偏振分量的强度Iss。 根据这些测量值，根据以下等式计算G因子：G = [（IppxIsp）/（IpsxIss）] + E，fra 1/2 + EE，并且基于该G因子来实现极化响应校正以获得极化 荧光特性。