公开(公告)号：US20240318235A1

公开(公告)日：2024-09-26

申请号：US18739955

申请日：2024-06-11

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12N9/10 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/53

CPC分类号： C12Q1/6827 ,  C12N9/1007 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/5308 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/724 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20210246494A1

公开(公告)日：2021-08-12

申请号：US17231671

申请日：2021-04-15

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12Q1/26 ,  G01N33/53 ,  C12Q1/6806 ,  C12Q1/6869

摘要： The present invention provides for methods of epigenetic analysis. In some cases, the methods may include obtaining a sample comprising a nucleic acid sequence. In some cases, the nucleic acid sequence may comprise one or more epigenetic marks. The methods may include performing a sequencing. The methods may include distinguishing a hydroxymethylated base from a methylated base.

公开(公告)号：US20200040381A1

公开(公告)日：2020-02-06

申请号：US16658195

申请日：2019-10-21

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12Q1/26 ,  G01N33/50 ,  C12N9/02 ,  G01N33/53 ,  C12N5/00 ,  C12Q1/6806 ,  C12N5/074 ,  G01N33/574 ,  C12N5/0783 ,  C12N15/873 ,  C12Q1/6869 ,  C12Q1/6886

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US10465234B2

公开(公告)日：2019-11-05

申请号：US16169801

申请日：2018-10-24

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/26 ,  C12Q1/6827 ,  G01N33/50 ,  C12N9/02 ,  G01N33/53 ,  C12N5/00 ,  C12Q1/6806 ,  C12N5/074 ,  G01N33/574 ,  C12N5/0783 ,  C12N15/873 ,  C12Q1/6869 ,  C12Q1/6886

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US10031131B2

公开(公告)日：2018-07-24

申请号：US15440284

申请日：2017-02-23

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： G01N33/574 ,  G01N33/53 ,  C12N5/074

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20240254541A1

公开(公告)日：2024-08-01

申请号：US18588930

申请日：2024-02-27

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12N9/10 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/53

CPC分类号： C12Q1/6827 ,  C12N9/1007 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/5308 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/724 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20220348995A1

公开(公告)日：2022-11-03

申请号：US17675502

申请日：2022-02-18

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/26 ,  G01N33/53 ,  C12N9/10 ,  C12Q1/6806

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US11072818B2

公开(公告)日：2021-07-27

申请号：US16380846

申请日：2019-04-10

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/26 ,  C12Q1/6827 ,  G01N33/53 ,  C12Q1/6806 ,  C12Q1/6869

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US10731204B2

公开(公告)日：2020-08-04

申请号：US15725917

申请日：2017-10-05

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/53

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US10508301B2

公开(公告)日：2019-12-17

申请号：US16012510

申请日：2018-06-19

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： G01N33/574 ,  G01N33/53 ,  C12N5/074 ,  C12N9/02 ,  C12Q1/6827 ,  C12Q1/26 ,  G01N33/50 ,  C12N5/00 ,  C12Q1/6806 ,  C12N5/0783 ,  C12N15/873 ,  C12Q1/6869 ,  C12Q1/6886

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.