公开(公告)号：US20240026031A1

公开(公告)日：2024-01-25

申请号：US17773283

申请日：2020-10-30

申请人： The Regents of the University of California

发明人： James A. WELLS ,  Adam D. COTTON ,  Duy NGUYEN ,  Katarina PANCE

IPC分类号： C07K16/40 ,  C07K16/28 ,  A61K47/68

CPC分类号： C07K16/40 ,  C07K16/2827 ,  A61K47/6879 ,  A61K47/6803 ,  C07K2317/55 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2317/92

摘要： The present disclosure relates to, among other things, methods for degrading targeted surface proteins using the ubiquitin pathway by using a bispecific binding agent or an immunoconjugate that binds the targeted surface protein and a membrane-associated ubiquitin E3 ligase. The present disclosure also relates to methods for degrading targeted surface proteins using the ubiquitin pathway using an engineered transmembrane protein that binds the targeted surface protein and exhibits ubiquitin E3 ligase activity. The disclosure also provides compositions and methods useful for producing such bispecific binding agents and engineered transmembrane proteins, immunoconjugates, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various diseases such as cancers.

公开(公告)号：US20230265206A1

公开(公告)日：2023-08-24

申请号：US18182277

申请日：2023-03-10

申请人： The Regents of the University of California

发明人： James A. WELLS ,  Alexander J. MARTINKO ,  Juan E. DIAZ ,  Michael J. HORNSBY

IPC分类号： C07K16/28 ,  A61K47/68 ,  A61P35/00 ,  G01N33/574

CPC分类号： C07K16/2896 ,  A61K47/6803 ,  A61K47/6849 ,  A61P35/00 ,  C07K16/2809 ,  G01N33/57492 ,  C07K2317/31 ,  C07K2317/92 ,  G01N2333/70596

摘要： The disclosure is related to antibodies and antibody fragments that specifically bind the CUB domain-containing protein 1 (CDCP1) and their methods and uses in treating and detecting cancers.

公开(公告)号：US20240092893A1

公开(公告)日：2024-03-21

申请号：US18359569

申请日：2023-07-26

申请人： The Regents of the University of California

发明人： Katarina PANCE ,  James A. WELLS

IPC分类号： C07K16/28 ,  C07K16/18 ,  C07K16/22 ,  C07K16/24 ,  C07K16/30 ,  C07K16/32 ,  C07K16/40

CPC分类号： C07K16/28 ,  C07K16/18 ,  C07K16/22 ,  C07K16/244 ,  C07K16/245 ,  C07K16/246 ,  C07K16/248 ,  C07K16/2803 ,  C07K16/2818 ,  C07K16/2827 ,  C07K16/2851 ,  C07K16/2863 ,  C07K16/2896 ,  C07K16/30 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/31

摘要： The present disclosure relates to targeted degradation platform technology. For example, the present disclosure relates to bispecific binding agents for degrading endogenous proteins, whether membrane-associated or soluble, using the lysosome pathway. The disclosure also provides methods useful for producing such agents, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various disorders.

公开(公告)号：US20220332776A1

公开(公告)日：2022-10-20

申请号：US17633743

申请日：2020-08-06

申请人： The Regents of the University of California

发明人： James A. WELLS ,  Zachary B. HILL ,  Alexander J. MARTINKO

IPC分类号： C07K14/47 ,  C12N9/22 ,  C07K16/28 ,  A61K38/46 ,  A61K38/17 ,  A61K39/395 ,  A61K35/17 ,  A61P37/02 ,  C07K16/44 ,  A61K31/454 ,  A61K31/711

摘要： The present invention provides antibody-based chemically induced dimerizers (AbCIDs) comprising an antibody domain that recognizes a chemical epitope created by the binding of an IMiD to a binding partner, such as cereblon, and methods to rapidly generate such AbCIDs. Several aspects described herein relate to systems, compositions, and methods including components of a chemically induced dimerizer (CID), such as an antibody chemically induced dimerizer (AbCID).

公开(公告)号：US20160362439A1

公开(公告)日：2016-12-15

申请号：US15118268

申请日：2015-02-13

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Adam R. RENSLO ,  Erica M.W. LAUTERWASSER ,  Shaun D. FONTAINE ,  Benjamin B. SPANGLER ,  James A. WELLS

IPC分类号： C07H19/16 ,  A61K31/357 ,  C07D405/12 ,  C07D405/14 ,  A61K31/4709 ,  A61K31/4192 ,  A61K31/404 ,  A61K31/513 ,  C07D407/12 ,  C07D491/113 ,  A61K31/7076 ,  C07D487/04 ,  A61K31/7135 ,  C07F7/18 ,  A61K31/662 ,  C07F9/655 ,  A61K31/7072 ,  C07H19/06 ,  A61K31/4745 ,  C07D491/153 ,  A61K31/427 ,  C07D493/14 ,  A61K47/48 ,  C07K7/06 ,  A61K31/437 ,  A61K31/4025 ,  C07D417/12 ,  A61K31/444 ,  A61K31/4985 ,  A61K51/04 ,  A61K31/496 ,  C07D417/14 ,  C07D491/107 ,  A61K31/435 ,  C07D323/02

CPC分类号： C07H19/16 ,  A61K31/357 ,  A61K31/4025 ,  A61K31/404 ,  A61K31/4192 ,  A61K31/427 ,  A61K31/435 ,  A61K31/437 ,  A61K31/444 ,  A61K31/4709 ,  A61K31/4745 ,  A61K31/496 ,  A61K31/4985 ,  A61K31/513 ,  A61K31/662 ,  A61K31/7072 ,  A61K31/7076 ,  A61K31/7135 ,  A61K47/64 ,  A61K47/6803 ,  A61K51/0459 ,  C07B2200/05 ,  C07D323/02 ,  C07D405/12 ,  C07D405/14 ,  C07D407/12 ,  C07D417/12 ,  C07D417/14 ,  C07D471/14 ,  C07D487/04 ,  C07D491/107 ,  C07D491/113 ,  C07D491/153 ,  C07D493/04 ,  C07D493/14 ,  C07D519/04 ,  C07F7/1804 ,  C07F9/65517 ,  C07H19/06 ,  C07H19/167 ,  C07K7/06 ,  Y02A50/411

摘要： Disclosed herein, inter alia, are prodrug compositions and methods of using the same for treatment and detection of disease. Specifically, disclosed herein is a compound of formula (I) having spiro-fused 1,2,4-trioxolane and piperidine rings, namely, 1,2,4-trioxa-8-azaspiro[4.5]decane. Also disclosed is a pharmaceutical composition containing the compound and a pharmaceutically acceptable carrier.

摘要翻译： 本文公开的是前药组合物及其用于治疗和检测疾病的方法。 具体地，本文公开了具有螺稠合的1,2,4-三恶烷和哌啶环的式（I）化合物，即1,2,4-三氧杂-8-氮杂螺[4.5]癸烷。 还公开了含有该化合物和药学上可接受的载体的药物组合物。

公开(公告)号：US20240189423A1

公开(公告)日：2024-06-13

申请号：US18553447

申请日：2022-03-30

申请人： The Regents of the University of California

发明人： James A. WELLS ,  Katarina PANCE ,  Josef A. GRAMESPACHER ,  Kaan Kumru

IPC分类号： A61K39/00 ,  A61K47/68 ,  A61P35/00 ,  C07K14/475 ,  C07K14/48 ,  C07K14/49 ,  C07K14/495 ,  C07K14/50 ,  C07K14/505 ,  C07K14/51 ,  C07K14/52 ,  C07K14/525 ,  C07K14/53 ,  C07K14/54 ,  C07K14/55 ,  C07K14/56 ,  C07K14/565 ,  C07K14/57 ,  C07K14/575 ,  C07K14/61 ,  C07K14/65 ,  C07K16/28 ,  C12N5/0781

CPC分类号： A61K39/4644 ,  A61K39/4612 ,  A61K39/4633 ,  A61K47/6851 ,  A61P35/00 ,  C07K14/475 ,  C07K14/48 ,  C07K14/49 ,  C07K14/495 ,  C07K14/50 ,  C07K14/505 ,  C07K14/51 ,  C07K14/521 ,  C07K14/525 ,  C07K14/53 ,  C07K14/5403 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5412 ,  C07K14/5418 ,  C07K14/5425 ,  C07K14/5431 ,  C07K14/5434 ,  C07K14/5437 ,  C07K14/55 ,  C07K14/56 ,  C07K14/565 ,  C07K14/57 ,  C07K14/575 ,  C07K14/61 ,  C07K14/65 ,  C07K16/2803 ,  C07K16/2887 ,  C12N5/0635 ,  C07K2317/31 ,  C07K2319/30 ,  C12N2510/00

摘要： The present disclosure relates to targeted degradation platform technology. For example, the present disclosure relates to bispecific binding agents for degrading endogenous proteins, whether membrane-associated or soluble, using the lysosome pathway. The disclosure also provides methods useful for producing such agents, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various disorders.

公开(公告)号：US20240084030A1

公开(公告)日：2024-03-14

申请号：US18359562

申请日：2023-07-26

申请人： The Regents of the University of California

发明人： Katarina PANCE ,  James A. WELLS

IPC分类号： C07K16/28 ,  A61P35/00

CPC分类号： C07K16/2896 ,  A61P35/00 ,  C07K16/2803 ,  C07K2317/31

摘要： The present disclosure relates to targeted degradation platform technology. For example, the present disclosure relates to bispecific binding agents for degrading endogenous proteins, whether membrane-associated or soluble, using the lysosome pathway. The disclosure also provides methods useful for producing such agents, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various disorders.

公开(公告)号：US20190185836A1

公开(公告)日：2019-06-20

申请号：US16329176

申请日：2017-08-31

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： James A. WELLS ,  Amy M. WEEKS

IPC分类号： C12N9/50 ,  C12Q1/37

CPC分类号： C12N9/50 ,  C12N9/54 ,  C12N9/93 ,  C12Q1/37 ,  C12Y304/21062

摘要： In various embodiments, the invention provides subtiligase variants that recognize an N-terminus of a protein or peptide substrate that is different than the N-terminus that is recognized by the corresponding wild-type subtiligase. Also provided are methods and kits for using such subtiligase variants to site-specifically ligate a synthetic molecule comprising a peptide ester to a protein or peptide substrate of interest.

公开(公告)号：US20190144937A1

公开(公告)日：2019-05-16

申请号：US16097140

申请日：2017-05-01

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  UNIVERSITY OF TORONTO

发明人： James A. WELLS ,  Samuel POLLOCK ,  Sachdev SIDHU ,  Jason MOFFAT

IPC分类号： C12Q1/6874 ,  C07K19/00

摘要： The present invention provides methods of identifying the presence and relative abundance of a protein in or on a cell or population of cells, with the methods comprising applying to a population of cellular proteins a collection of Fab-phage particles that contain nucleic acid encoding at least one antibody Fab fragment, wherein each of the antibody Fab fragments has a known protein to which it will bind in a specific manner. After binding is allowed to occur, those Fab-phage not bound to targets are washed away and the remaining phage are propagated in bacteria before the nucleic acid within the Fab-phage is amplified and then sequenced to determine the polynucleotide sequences of the nucleic acid molecules from the Fab-phages that bound to the cellular proteins. The nucleotide sequences of the nucleic acid molecules from the Fab-phages correlate to the coding sequences of the antibody Fab fragments that are known to bind in a specific manner to a protein.

公开(公告)号：US20240327523A1

公开(公告)日：2024-10-03

申请号：US18034234

申请日：2021-11-15

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： LEWIS L. LANIER ,  James A. WELLS ,  Jia LIU ,  Janice ARAKAWA-HOYT

IPC分类号： C07K16/28 ,  A61K47/68 ,  A61P35/00

CPC分类号： C07K16/2851 ,  A61K47/6803 ,  A61K47/6849 ,  A61P35/00 ,  C07K16/2818 ,  C07K16/2827 ,  C07K2317/21 ,  C07K2317/24

摘要： Anti-CD94 antibodies and their use are described.