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公开(公告)号:US20220364167A1
公开(公告)日:2022-11-17
申请号:US17860036
申请日:2022-07-07
发明人: Jingyue Ju , Shiv Kumar , James J. Russo , Steffen Jockusch , Zengmin Li , Xiaoxu Li , Sergey M. Kalachikov , Irina Morozova
IPC分类号: C12Q1/6869 , C07H19/10 , C07H19/20
摘要: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded
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公开(公告)号:US20200069717A1
公开(公告)日:2020-03-05
申请号:US16303715
申请日:2017-05-23
发明人: Jingyue Ju , Xin Chen , Xiaoxu Li , Zengmin Li , Min-Kang Hsieh , Minchen Chien , Shundi Shi , Jianyi Ren , Cheng Guo , Shiv Kumar , James J. Russo , Chuanjuan Tao , Steffen Jockusch , Sergey Kalachikov
IPC分类号: A61K31/7052 , C07H21/04
摘要: Disclosed herein, inter alia, are compounds, compositions, and methods of thereof in the sequencing a nucleic acid.
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公开(公告)号:US20190153527A1
公开(公告)日:2019-05-23
申请号:US16091442
申请日:2017-04-04
发明人: Jingyue Ju , Shiv Kumar , James J. Russo , Steffen Jockusch , Zengmin Li , Xiaoxu Li , Sergey M. Kalachikov , Irina Morozova
IPC分类号: C12Q1/6869 , C07H19/10 , C07H19/20
摘要: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded
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公开(公告)号:US20190085014A1
公开(公告)日:2019-03-21
申请号:US16149114
申请日:2018-10-01
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6872 , C12Q1/6876 , C07H19/10 , C12Q1/68 , C12Q1/6874 , C40B40/00
CPC分类号: C12Q1/6869 , C07B2200/11 , C07H19/10 , C07H19/14 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2525/186 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C12Q2565/501 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20190031705A1
公开(公告)日:2019-01-31
申请号:US16150185
申请日:2018-10-02
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/6869 , C07H19/10 , C12Q1/6874 , C12Q1/6876 , C12Q1/6872 , C12Q1/68 , C12Q1/686
CPC分类号: C12Q1/6869 , C07B2200/11 , C07H19/10 , C07H19/14 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2525/186 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C12Q2565/501 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US09725480B2
公开(公告)日:2017-08-08
申请号:US15167917
申请日:2016-05-27
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
CPC分类号: C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20220290227A1
公开(公告)日:2022-09-15
申请号:US17576184
申请日:2022-01-14
发明人: Jingyue Ju , Dae Hyun Kim , Jia Guo , Qinglin Meng , Zengmin Li , Huanyan Cao
IPC分类号: C12Q1/6874 , C07H19/04 , C12Q1/6869 , C12Q1/6876
摘要: This invention provides a process for sequencing nucleic acids using 3′ modified deoxynucleotide analogues or 3′ modified deoxyinosine triphosphate analogues, and 3′ modified dideoxynucleotide analogues having a detectable marker attached thereto.
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公开(公告)号:US11266673B2
公开(公告)日:2022-03-08
申请号:US16303715
申请日:2017-05-23
发明人: Jingyue Ju , Xin Chen , Xiaoxu Li , Zengmin Li , Min-Kang Hsieh , Minchen Chien , Shundi Shi , Jianyi Ren , Cheng Guo , Shiv Kumar , James J. Russo , Chuanjuan Tao , Steffen Jockusch , Sergey Kalachikov
IPC分类号: C12Q1/68 , A61K31/7052 , C07H21/04 , C07H19/20 , C12Q1/6869
摘要: Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing a nucleic acid.
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公开(公告)号:US11225687B2
公开(公告)日:2022-01-18
申请号:US16091435
申请日:2017-04-04
发明人: Jingyue Ju , Shiv Kumar , James J. Russo , Steffen Jockusch , Zengmin Li , Xiaoxu Li , Sergey Kalachikov , Irina Morozova
IPC分类号: C12Q1/68 , C12Q1/6869 , C12N9/12 , B82Y5/00 , B82Y30/00 , C12Q1/6825 , G01N21/65
摘要: This invention provides nucleoside polyphosphate analogues each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs. This invention also provides nucleotide polymerases with one or more attached and/or conjugated noble metal nanoparticles, wherein the noble metal nanoparticles are surface-enhanced Raman spectroscopy (SERS) substrates thereby creating a region of enhanced sensitivity for surface enhanced Raman spectroscopy (SERS) within or adjacent to the polymerase. This invention also provides a surface with regions of enhanced sensitivity for surface enhanced Raman spectroscopy comprising interspersed rough or nanostructured noble metal surface. This invention also provides methods for determining the sequence of a single stranded DNA or RNA polynucleotide using one or more of nucleoside polyphosphate analogues, polymerase with noble metal nanoparticles, and surface with noble metal.
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公开(公告)号:US10662472B2
公开(公告)日:2020-05-26
申请号:US16200557
申请日:2018-11-26
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C12Q1/68 , C07H19/10 , C07H19/14 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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