公开(公告)号：US06947845B2

公开(公告)日：2005-09-20

申请号：US10211931

申请日：2002-08-02

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Joseph A. Ippolito ,  Nenad Ban ,  Poul Nissen ,  Jeffrey L. Hansen

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Joseph A. Ippolito ,  Nenad Ban ,  Poul Nissen ,  Jeffrey L. Hansen

IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K31/165 ,  A61K31/40 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K31/7064 ,  A61K45/06 ,  A61K48/00 ,  C07H21/04 ,  C07K14/215 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/68 ,  G06F17/30 ,  G06F17/50 ,  G01N33/48 ,  G06F31/00 ,  G06F17/00

CPC分类号： A61K31/00 ,  A61K31/165 ,  A61K31/40 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K31/7064 ,  A61K45/06 ,  C07D239/54 ,  C07D403/12 ,  C07K14/215 ,  C07K2299/00 ,  G01N33/6872 ,  G01N2500/00 ,  Y02A90/26

摘要： The invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as crystals produced by such methods. The invention also provides high resolution structures of ribosomal subunits either alone or in combination with protein synthesis inhibitors. The invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties as well as ligands that may act as protein synthesis inhibitors. Thus, the methods and compositions of the invention may be used to produce ligands that are designed to specifically kill or inhibit the growth of any target organism.

摘要翻译： 本发明提供了生产核糖体和核糖体亚基的高分辨率晶体以及通过这些方法产生的晶体的方法。 本发明还单独或与蛋白质合成抑制剂组合提供核糖体亚基的高分辨率结构。 本发明提供了用于鉴定核糖体相关配体的方法和用于设计具有特定核糖体结合特性的配体的方法以及可用作蛋白质合成抑制剂的配体。 因此，本发明的方法和组合物可用于产生设计用于特异性杀死或抑制任何靶生物体生长的配体。

公开(公告)号：US20100312525A1

公开(公告)日：2010-12-09

申请号：US12703721

申请日：2010-02-10

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen ,  Joseph A. Ippolito

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen ,  Joseph A. Ippolito

IPC分类号： G06F17/50

CPC分类号： A61K31/00 ,  A61K31/165 ,  A61K31/40 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K31/7064 ,  A61K45/06 ,  C07D239/54 ,  C07D403/12 ,  C07K14/215 ,  C07K2299/00 ,  G01N33/6872 ,  G01N2500/00 ,  Y02A90/26

摘要： The invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as crystals produced by such methods. The invention also provides high resolution structures of ribosomal subunits either alone or in combination with protein synthesis inhibitors. The invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties as well as ligands that may act as protein synthesis inhibitors. Thus, the methods and compositions of the invention may be used to produce ligands that are designed to specifically kill or inhibit the growth of any target organism.

摘要翻译： 本发明提供了生产核糖体和核糖体亚基的高分辨率晶体以及通过这些方法产生的晶体的方法。 本发明还单独或与蛋白质合成抑制剂组合提供核糖体亚基的高分辨率结构。 本发明提供了用于鉴定核糖体相关配体的方法和用于设计具有特定核糖体结合特性的配体的方法以及可用作蛋白质合成抑制剂的配体。 因此，本发明的方法和组合物可用于产生设计用于特异性杀死或抑制任何靶生物体生长的配体。

公开(公告)号：US06952650B2

公开(公告)日：2005-10-04

申请号：US10072634

申请日：2002-02-08

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen ,  Joseph A. Ippolito

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen ,  Joseph A. Ippolito

IPC分类号： A61K31/165 ,  A61K31/40 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K31/7064 ,  A61K45/06 ,  C07K14/215 ,  G01N33/48 ,  G06F31/00 ,  G06F17/00 ,  C12Q1/68

CPC分类号： A61K45/06 ,  A61K31/165 ,  A61K31/40 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K31/7064 ,  C07K14/215 ,  C07K2299/00 ,  Y02A90/26

摘要： The invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as crystals produced by such methods. The invention also provides high resolution structures of ribosomal subunits either alone or in combination with protein synthesis inhibitors. The invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties as well as ligands that may act as protein synthesis inhibitors. Thus, the methods and compositions of the invention may be used to produce ligands that are designed to specifically kill or inhibit the growth of any target organism.

公开(公告)号：US06638908B1

公开(公告)日：2003-10-28

申请号：US09635708

申请日：2000-08-09

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

IPC分类号： A61K3800

CPC分类号： C07K14/215 ,  C07K2299/00

摘要： The present invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as the crystals produced by such methods. The x-ray diffraction patterns of the crystals provided by the present invention are of sufficiently high resolution for determining the three-dimensional structure of ribosomes and ribosomal subunits, for identifying ligand binding sites on ribosomes and ribosomal subunits, and for molecular modeling of ligands which interact with ribosomes and ribosomal subunits. The present invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties. Thus, the methods of the present invention may be used to produce ligands which are designed to kill or inhibit any specific target organism(s).

摘要翻译： 本发明提供了生产核糖体和核糖体亚基的高分辨率晶体的方法以及通过这些方法制备的晶体。 由本发明提供的晶体的x射线衍射图具有足够高的分辨率，用于确定核糖体和核糖体亚基的三维结构，用于鉴定核糖体和核糖体亚基上的配体结合位点，以及用于配体的分子模拟 与核糖体和核糖体亚基相互作用。 本发明提供鉴定核糖体相关配体的方法和用于设计具有特定核糖体结合特性的配体的方法。 因此，本发明的方法可用于产生设计用于杀死或抑制任何特定靶生物的配体。

公开(公告)号：US20100131258A1

公开(公告)日：2010-05-27

申请号：US12220342

申请日：2008-07-22

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

IPC分类号： G06G7/48

CPC分类号： A61K31/40 ,  A61K31/165 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K45/06 ,  C07K14/215 ,  C07K2299/00

摘要： The invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as crystals produced by such methods. The invention also provides high resolution structures of ribosomal subunits either alone or in combination with protein synthesis inhibitors. The invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties as well as ligands that may act as protein synthesis inhibitors. Thus, the methods and compositions of the invention may be used to produce ligands that are designed to specifically kill or inhibit the growth of any target organism.

摘要翻译： 本发明提供了生产核糖体和核糖体亚基的高分辨率晶体以及通过这些方法产生的晶体的方法。 本发明还单独或与蛋白质合成抑制剂组合提供核糖体亚基的高分辨率结构。 本发明提供了用于鉴定核糖体相关配体的方法和用于设计具有特定核糖体结合特性的配体的方法以及可用作蛋白质合成抑制剂的配体。 因此，本发明的方法和组合物可用于产生设计用于特异性杀死或抑制任何靶生物体生长的配体。

公开(公告)号：US07504486B2

公开(公告)日：2009-03-17

申请号：US10391289

申请日：2003-03-17

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

IPC分类号： C07K1/14 ,  G01N31/00

CPC分类号： C07K14/215 ,  C07K2299/00

摘要： The present invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as the crystals produced by such methods. The x-ray diffraction patterns of the crystals provided by the present invention are of sufficiently high resolution for determining the three-dimensional structure of ribosomes and ribosomal subunits, for identifying ligand binding sites on ribosomes and ribosomal subunits, and for molecular modeling of ligands which interact with ribosomes and ribosomal subunits. The present invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties. Thus, the methods of the present invention may be used to produce ligands which are designed to kill or inhibit any specific target organism(s).

摘要翻译： 本发明提供了生产核糖体和核糖体亚基的高分辨率晶体的方法以及通过这些方法制备的晶体。 由本发明提供的晶体的x射线衍射图具有足够高的分辨率，用于确定核糖体和核糖体亚基的三维结构，用于鉴定核糖体和核糖体亚基上的配体结合位点，以及用于配体的分子模拟 与核糖体和核糖体亚基相互作用。 本发明提供鉴定核糖体相关配体的方法和用于设计具有特定核糖体结合特性的配体的方法。 因此，本发明的方法可用于产生设计用于杀死或抑制任何特定靶生物的配体。

公开(公告)号：US06947844B2

公开(公告)日：2005-09-20

申请号：US09922251

申请日：2001-08-03

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

IPC分类号： G01N33/50 ,  A61K31/165 ,  A61K31/40 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K45/06 ,  C07K1/14 ,  C07K14/195 ,  C07K14/215 ,  G01N33/15 ,  G01N33/48 ,  G06F17/00 ,  G06F31/00

CPC分类号： A61K31/40 ,  A61K31/165 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K45/06 ,  C07K14/215 ,  C07K2299/00

摘要： The invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as crystals produced by such methods. The invention also provides high resolution structures of ribosomal subunits either alone or in combination with protein synthesis inhibitors. The invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties as well as ligands that may act as protein synthesis inhibitors. Thus, the methods and compositions of the invention may be used to produce ligands that are designed to specifically kill or inhibit the growth of any target organism.

摘要翻译： 本发明提供了生产核糖体和核糖体亚基的高分辨率晶体以及通过这些方法产生的晶体的方法。 本发明还单独或与蛋白质合成抑制剂组合提供核糖体亚基的高分辨率结构。 本发明提供了用于鉴定核糖体相关配体的方法和用于设计具有特定核糖体结合特性的配体的方法以及可用作蛋白质合成抑制剂的配体。 因此，本发明的方法和组合物可用于产生设计用于特异性杀死或抑制任何靶生物体生长的配体。

公开(公告)号：US06939848B2

公开(公告)日：2005-09-06

申请号：US10391491

申请日：2003-03-17

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Nenad Ban ,  Poul Nissen ,  Jeffrey Hansen

IPC分类号： C07K14/215 ,  A61K38/00 ,  G01N33/48 ,  G06F17/00 ,  G06F19/00

CPC分类号： C07K14/215 ,  C07K2299/00

摘要： The present invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as the crystals produced by such methods. The x-ray diffraction patterns of the crystals provided by the present invention are of sufficiently high resolution for determining the three-dimensional structure of ribosomes and ribosomal subunits, for identifying ligand binding sites on ribosomes and ribosomal subunits, and for molecular modeling of ligands which interact with ribosomes and ribosomal subunits. The present invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties. Thus, the methods of the present invention may be used to produce ligands which are designed to kill or inhibit any specific target organism(s).

摘要翻译： 本发明提供了生产核糖体和核糖体亚基的高分辨率晶体的方法以及通过这些方法制备的晶体。 由本发明提供的晶体的x射线衍射图具有足够高的分辨率，用于确定核糖体和核糖体亚基的三维结构，用于鉴定核糖体和核糖体亚基上的配体结合位点，以及用于配体的分子模拟 与核糖体和核糖体亚基相互作用。 本发明提供鉴定核糖体相关配体的方法和用于设计具有特定核糖体结合特性的配体的方法。 因此，本发明的方法可用于产生设计用于杀死或抑制任何特定靶生物的配体。

公开(公告)号：US08470990B2

公开(公告)日：2013-06-25

申请号：US12645877

申请日：2009-12-23

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Joyce A. Sutcliffe ,  Adegboyega K. Oyelere ,  Joseph A. Ippolito

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Joyce A. Sutcliffe ,  Adegboyega K. Oyelere ,  Joseph A. Ippolito

IPC分类号： C07H21/04 ,  C12N15/11

CPC分类号： A61K31/00 ,  A61K31/165 ,  A61K31/40 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K31/7064 ,  A61K45/06 ,  C07D239/54 ,  C07D403/12 ,  C07K14/215 ,  C07K2299/00 ,  G01N33/6872 ,  G01N2500/00 ,  Y02A90/26

摘要： The invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as crystals produced by such methods. The invention also provides high resolution structures of ribosomal subunits either alone or in combination with protein synthesis inhibitors. The invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties as well as ligands that may act as protein synthesis inhibitors. Thus, the methods and compositions of the invention may be used to produce ligands that are designed to specifically kill or inhibit the growth of any target organism.

公开(公告)号：US07666849B2

公开(公告)日：2010-02-23

申请号：US11067522

申请日：2005-02-25

申请人： Thomas A. Steitz ,  Peter B. Moore ,  Joyce A. Sutcliffe ,  Adegboyega K. Oyelere ,  Joseph A. Ippolito

发明人： Thomas A. Steitz ,  Peter B. Moore ,  Joyce A. Sutcliffe ,  Adegboyega K. Oyelere ,  Joseph A. Ippolito

IPC分类号： A61K38/00

CPC分类号： A61K31/00 ,  A61K31/165 ,  A61K31/40 ,  A61K31/506 ,  A61K31/513 ,  A61K31/5355 ,  A61K31/7048 ,  A61K31/7064 ,  A61K45/06 ,  C07D239/54 ,  C07D403/12 ,  C07K14/215 ,  C07K2299/00 ,  G01N33/6872 ,  G01N2500/00 ,  Y02A90/26

摘要： The invention provides methods for producing high resolution crystals of ribosomes and ribosomal subunits as well as crystals produced by such methods. The invention also provides high resolution structures of ribosomal subunits either alone or in combination with protein synthesis inhibitors. The invention provides methods for identifying ribosome-related ligands and methods for designing ligands with specific ribosome-binding properties as well as ligands that may act as protein synthesis inhibitors. Thus, the methods and compositions of the invention may be used to produce ligands that are designed to specifically kill or inhibit the growth of any target organism.

摘要翻译： 本发明提供了生产核糖体和核糖体亚基的高分辨率晶体以及通过这些方法产生的晶体的方法。 本发明还单独或与蛋白质合成抑制剂组合提供核糖体亚基的高分辨率结构。 本发明提供了用于鉴定核糖体相关配体的方法和用于设计具有特定核糖体结合特性的配体的方法以及可用作蛋白质合成抑制剂的配体。 因此，本发明的方法和组合物可用于产生设计用于特异性杀死或抑制任何靶生物体生长的配体。