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公开(公告)号：US6107083A
公开(公告)日：2000-08-22
申请号：US137616
申请日：1998-08-21
CPC分类号： G01N33/525 , C12Q1/26 , C12Q1/54 , G01N2333/904 , G01N2333/906 , G01N2333/98 , G01N2333/986
摘要： The invention discloses enzyme-based optical sensors for detecting blood components which are substrates for oxidative enzymes, the sensors advantageously employing a multiple-layer structure featuring a thin, rapidly responding, optical, oxygen sensing layer. The sensors comprise, in order, a) an enzymatic layer containing an oxidative enzyme or enzyme cascade in a water and oxygen-permeable matrix; b) an oxygen sensing layer containing luminescent dye in a light-transmissive, oxygen-permeable matrix; and which is preferably deposited onto c) a light-transmissive substrate. Embodiments of the invention may further include a rapidly hydrating gas-permeable cover, or spacer, layer deposited over the enzymatic layer. A particulate filler material may be included in an effective amount in the oxygen sensing layer to reduce sample light scattering effects. The sensors of the disclosure may be used to detect creatinine and other enzyme-oxidizable analytes such as glucose, lactate or cholesterol, and may be made easily using standard coating techniques known in the art. These sensors are suitable for multiple analyses, but the materials and methods used allow the sensors to be disposable as well, allowing their wide use in medical and analytical applications.
摘要翻译： 本发明公开了用于检测作为氧化酶底物的血液成分的基于酶的光学传感器，该传感器有利地采用具有薄的，快速响应的光学氧传感层的多层结构。 传感器依次包括a）在水和氧气可渗透基质中含有氧化酶或酶级联的酶层; b）在透光，透氧的基质中含有发光染料的氧气感测层; 并且其优选沉积在c）透光基底上。 本发明的实施方案还可以包括在酶层上沉积的快速水合的透气性覆盖物或间隔物层。 颗粒填料可以以有效量包含在氧气感测层中以减少样品光散射效应。 本公开的传感器可用于检测肌酐和其它可酶氧化分析物如葡萄糖，乳酸或胆固醇，并且可以使用本领域已知的标准涂层技术容易地制备。 这些传感器适用于多种分析，但所使用的材料和方法可使传感器成为一次性使用，可广泛应用于医疗和分析应用。
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公开(公告)号：US06254831B1
公开(公告)日：2001-07-03
申请号：US09009917
申请日：1998-01-21
申请人： Steven M. Barnard , Thomas C. Collins , Susan L. Cudmore , Richard W. Mason , Christiane Munkholm , Rudolf E. Slovacek , Kevin J. Sullivan
发明人： Steven M. Barnard , Thomas C. Collins , Susan L. Cudmore , Richard W. Mason , Christiane Munkholm , Rudolf E. Slovacek , Kevin J. Sullivan
IPC分类号： G01N2164
CPC分类号： G01N21/6428 , G01N2021/6432
摘要： Provided is an optical sensor including a support and a detection layer, wherein the detection layer includes: (a) a luminescent material wherein the luminescence intensity of the luminescent material varies as the amount of an analyte varies; (b) a reflective material having a highly efficient reflectance of the wavelengths of excitation and of emission of the luminescent material; and (c) a polymeric binder to support and hold together the luminescent material and the reflective material. Such an optical sensor can be advantageously used in the detection of gaseous, ionic, and nonionic analytes in highly scattering samples. Also provided are methods for the manufacture of such optical sensors.
摘要翻译： 提供了一种包括支撑体和检测层的光学传感器，其中检测层包括：（a）发光材料，其中发光材料的发光强度随着分析物的量的变化而变化;（b）反射材料，其具有 发光材料的激发波长和发射波长的高效反射率; 和（c）聚合物粘合剂，用于支撑和保持发光材料和反射材料。 这种光学传感器可以有利地用于高分散样品中气态，离子和非离子分析物的检测。 还提供了用于制造这种光学传感器的方法。
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公开(公告)号：US06207110B1
公开(公告)日：2001-03-27
申请号：US09137728
申请日：1998-08-21
IPC分类号： G01N2164
CPC分类号： G01N21/6428 , G01N2021/7786
摘要： A liquid permeable metallic coating is utilized in conjunction with a fluorescence based optical sensor. The metallic coating is deposited directly on, and is in physical contact with, the sensing membrane. The metallic coating does not require an intervening substrate layer or other components. When light from a light source is shone through the substantially light transmissive substrate onto the sensing membrane, the metallic overcoating reflects back the excitation light as well as the fluorescence light generated by the sensor such that substantially no light reaches the sample where the light may be scattered and/or absorbed by the sample. Accordingly, the accuracy and repeatability of the sensor is improved while the cost and production times associated with manufacturing the sensor are minimized.
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公开(公告)号：US06368869B2
公开(公告)日：2002-04-09
申请号：US09775332
申请日：2001-01-31
IPC分类号： G01N2164
CPC分类号： G01N21/6428 , G01N2021/7786
摘要： A liquid permeable metallic coating is utilized in conjunction with a fluorescence based optical sensor. The metallic coating is deposited directly on, and is in physical contact with, the sensing membrane. The metallic coating does not require an intervening substrate layer or other components. When light from a light source is shone through the substantially light transmissive substrate onto the sensing membrane, the metallic overcoating reflects back the excitation light as well as the fluorescence light generated by the sensor such that substantially no light reaches the sample where the light may be scattered and/or absorbed by the sample. Accordingly, the accuracy and repeatability of the sensor is improved while the cost and production times associated with manufacturing the sensor are minimized.
摘要翻译： 液体可渗透的金属涂层与荧光基光学传感器结合使用。 金属涂层直接沉积在感测膜上并与传感膜物理接触。 金属涂层不需要中间衬底层或其它部件。 当来自光源的光通过基本上透光的基底照射到感测膜上时，金属外涂层反射激发光以及由传感器产生的荧光，使得基本上没有光到达光可以是样品 被样品分散和/或吸收。 因此，传感器的精度和重复性得到改善，同时与生产传感器相关的成本和生产时间最小化。
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公开(公告)号：US06190612B1
公开(公告)日：2001-02-20
申请号：US09010161
申请日：1998-01-21
IPC分类号： G01N2164
CPC分类号： G01N31/225
摘要： Optical sensor formulations comprising polymeric sensing formulations and methods of predictably making optical sensor formulations, for, e.g., measuring O2 levels in patient blood samples. These formulations may be, e.g., deposited as a membrane on light-transmissive substrates. In an embodiment, O2-sensing formulations may be made by a process including selecting a first homopolymer comprised of first monomeric units, the first homopolymer having a first PermO2 value; selecting a second homopolymer comprised of second monomeric units, the second homopolymer having a second PermO2 value that is different from the first PermO2 value; and copolymerizing the first and second monomeric units to obtain a copolymer having an intermediate PermO2 value, i.e., between the two PermO2 values, the intermediate PermO2 providing the desired PermO2 for the desired oxygen sensing formulation. By adjusting the relative amounts of the first and second monomeric units, a series of polymers having a desired range of intermediate permeability values (and hence Stem-Volmer characteristic kSV values) may be obtained.
摘要翻译： 包括聚合物感测配方的光学传感器配方和可预测地制造光学传感器配方的方法，例如测量患者血液样品中的O 2水平。 这些制剂可以例如在透光性基材上作为膜沉积。 在一个实施方案中，O 2感测制剂可以通过包括选择由第一单体单元组成的第一均聚物，第一均聚物具有第一PermO 2值的方法制备; 选择由第二单体单元组成的第二均聚物，所述第二均聚物具有不同于第一PermO 2值的第二PermO 2值; 并且使第一和第二单体单元共聚以获得具有中等PermO 2值，即两个PermO 2值之间的中间体PermO 2，为所需的氧气感测配方提供期望的PermO 2的共聚物。 通过调节第一和第二单体单元的相对量，可以获得具有期望范围的中间渗透率值（并因此具有Stem-Volmer特征kSV值）的一系列聚合物。
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