-
公开(公告)号：US20050089859A1
公开(公告)日：2005-04-28
申请号：US10493925
申请日：2002-11-06
申请人： Thorsten Singer , Martin Weber
发明人： Thorsten Singer , Martin Weber
CPC分类号： C12N15/1006
摘要： The present invention relates to a simplified fast process for isolating nucleic acids, particularly plasmid DNA from E. coli.
摘要翻译： 本发明涉及用于从大肠杆菌分离核酸，特别是质粒DNA的简化快速方法。
-
公开(公告)号：US08685742B2
公开(公告)日：2014-04-01
申请号：US11572076
申请日：2005-07-15
申请人： Thorsten Singer
发明人： Thorsten Singer
IPC分类号： B01D37/00
CPC分类号： B01D15/361 , B01D15/125 , B01J20/28033 , B01J20/28078 , B01J20/28085 , B01J2220/86 , C12N15/1017 , G01N1/405 , G01N30/96 , G01N2030/527 , Y10T436/10 , Y10T436/178459
摘要： The present invention relates to an apparatus and a method for the more efficient cleaning of nucleic acids, and to a kit for carrying out this method.
摘要翻译： 本发明涉及用于更有效地清洁核酸的装置和方法，以及用于实施该方法的试剂盒。
-
公开(公告)号：US20120132853A1
公开(公告)日：2012-05-31
申请号：US13321468
申请日：2010-05-20
申请人： Thorsten Singer
发明人： Thorsten Singer
IPC分类号： C09K3/00
CPC分类号： B01D71/027 , B01D67/0088 , B01D67/0097 , B01D69/02
摘要： The present invention relates to a method of protecting membranes by treatment with an aqueous solution containing at least one water-soluble, nucleophilic compound, and the use of this aqueous solution for protecting matrices.
摘要翻译： 本发明涉及一种通过用含有至少一种水溶性亲核化合物的水溶液进行处理以及使用该水溶液保护基质来保护膜的方法。
-
4.发明授权公开(公告)号：US09458452B2
公开(公告)日：2016-10-04
申请号：US13884416
申请日：2011-11-08
申请人： Thorsten Singer , Holger Wedler
发明人： Thorsten Singer , Holger Wedler
CPC分类号： C12N15/101 , B01D15/34 , B01D15/3828 , C07H1/08
摘要： The invention relates to a chromatographic device for isolating and/or purifying double-stranded nucleic acids, preferably double-stranded DNA, from a mixture of such nucleic acids with single-stranded nucleic acids, oligonucleotides, mononucleotides, salts and/or other such impurities. The invention also relates to a method for chromatographically isolating and/or purifying same, and to a kit for this purpose.
摘要翻译： 本发明涉及用于从这种核酸与单链核酸，寡核苷酸，单核苷酸，盐和/或其它这些杂质的混合物中分离和/或纯化双链核酸，优选双链DNA的色谱装置 。 本发明还涉及用于色谱分离和/或纯化的方法以及用于该目的的试剂盒。
-
公开(公告)号：US20080113348A1
公开(公告)日：2008-05-15
申请号：US11572085
申请日：2005-07-15
申请人： Thorsten Singer
发明人： Thorsten Singer
CPC分类号： C12N15/101
摘要： The present invention relates to a method for cleaning and isolating nucleic acids using cationic detergents with the general formula (I): Y+R1R2R3R4X− (I) where Y can represent nitrogen or phosphorus R1, R2, R3 and R4 can represent independently from one another an unbranched or branched C1-C20-alkyl residue, C3-C6-alkenyl residue, C3-C6-alkinyl residue and/or a C6-C20-aryl residue as well as a C6-C26-aralkyl residue, and X— can represent an anion of an inorganic or organic single or multi-basic acid.
摘要翻译： 本发明涉及一种使用具有通式（I）的阳离子去污剂来清洗和分离核酸的方法：<？在线公式描述=“在线公式”end =“lead”→> 其中R 1，R 2，R 2，R 3，R 3，R 3，R 3， （I）<？in-line-formula description =“In-line formula”end =“tail”？>其中Y可以表示氮或磷R 1，R 2 ，R 3和R 4可以彼此独立地表示无支链或支链C 1 -C 20烷基， - 烷基残基，C 3 -C 6 - 链烯基残基，C 3 -C 6 - 炔基残基和C 3 -C 6 - /或C 6 -C 20 - 芳基以及C 6 -C 26 - 芳烷基 残基和X-可以代表无机或有机单碱或多碱的阴离子。
-
公开(公告)号：US20080039619A1
公开(公告)日：2008-02-14
申请号：US11572076
申请日：2005-07-15
申请人： Thorsten Singer
发明人： Thorsten Singer
CPC分类号： B01D15/361 , B01D15/125 , B01J20/28033 , B01J20/28078 , B01J20/28085 , B01J2220/86 , C12N15/1017 , G01N1/405 , G01N30/96 , G01N2030/527 , Y10T436/10 , Y10T436/178459
摘要： The present invention relates to an apparatus and a method for the more efficient cleaning of nucleic acids, and to a kit for carrying out this method.
摘要翻译： 本发明涉及用于更有效地清洁核酸的装置和方法，以及用于实施该方法的试剂盒。
-
公开(公告)号：US09458494B2
公开(公告)日：2016-10-04
申请号：US13703205
申请日：2011-06-14
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： Provided is a method for extracting nucleic acids from a wax-embedded sample, and use of particular solvents for removing wax from a wax-embedded sample for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample.
摘要翻译： 本发明提供从蜡嵌入样品中提取核酸的方法，使用特定溶剂从蜡嵌入样品中除去蜡，从交联的蜡嵌入样品中提取，分离和/或纯化核酸。
-
8.发明授权Method for cleaning and isolating nucleic acid using ammonium or phosphonium cationic detergents 有权使用铵或鏻阳离子洗涤剂清洗和分离核酸的方法公开(公告)号：US08679744B2
公开(公告)日：2014-03-25
申请号：US11572085
申请日：2005-07-15
申请人： Thorsten Singer
发明人： Thorsten Singer
CPC分类号： C12N15/101
摘要： The present invention relates to a method for cleaning and isolating nucleic acids using cationic detergents with the general formula (I): Y+R1R2R3R4X− (I) where Y can represent nitrogen or phosphorus R1, R2, R3 and R4 can represent independently from one another an unbranched or branched C1-C20-alkyl residue, C3-C6-alkenyl residue, C3-C6-alkinyl residue and/or a C6-C20-aryl residue as well as a C6-C26-aralkyl residue, and X— can represent an anion of an inorganic or organic single or multi-basic acid.
摘要翻译： 本发明涉及使用具有通式（I）的阳离子洗涤剂清洗和分离核酸的方法：Y + R1R2R3R4X-（I）其中Y可以表示氮或磷R1，R2，R3和R4可以独立地代表一个 另一个非支链或支链C 1 -C 20烷基残基，C 3 -C 6 - 烯基残基，C 3 -C 6 - 链烷基残基和/或C 6 -C 20芳基残基以及C 6 -C 26 - 芳烷基残基，以及X- 代表无机或有机单碱或多碱的阴离子。
-
公开(公告)号：US20130280787A1
公开(公告)日：2013-10-24
申请号：US13703205
申请日：2011-06-14
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： The present invention relates to a method for extracting nucleic acids from a wax-embedded sample, the use of particular solvents for removing wax from a wax-embedded sample in a method for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample as well as to a kit for extracting, isolating and/or purifying nucleic acids from a wax-embedded sample.
摘要翻译： 本发明涉及一种从嵌入蜡的样品中提取核酸的方法，在从交联的蜡 - 样品中提取，分离和/或纯化核酸的方法中使用特定溶剂从蜡包埋的样品中除去蜡， 嵌入样品以及用于从蜡嵌入样品中提取，分离和/或纯化核酸的试剂盒。
-
10.发明申请公开(公告)号：US20130237699A1
公开(公告)日：2013-09-12
申请号：US13884416
申请日：2011-11-08
申请人： Thorsten Singer , Holger Wedler
发明人： Thorsten Singer , Holger Wedler
CPC分类号： C12N15/101 , B01D15/34 , B01D15/3828 , C07H1/08
摘要： The invention relates to a chromatographic device for isolating and/or purifying double-stranded nucleic acids, preferably double-stranded DNA, from a mixture of such nucleic acids with single-stranded nucleic acids, oligonucleotides, mononucleotides, salts and/or other such impurities. The invention also relates to a method for chromatographically isolating and/or purifying same, and to a kit for this purpose.
摘要翻译： 本发明涉及用于从这种核酸与单链核酸，寡核苷酸，单核苷酸，盐和/或其它这些杂质的混合物中分离和/或纯化双链核酸，优选双链DNA的色谱装置 。 本发明还涉及用于色谱分离和/或纯化的方法以及用于该目的的试剂盒。
-
-
-
-
-
-
-
-
-
搜索结果
11 条记录 (耗时 0.019 秒)
