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    HAFNIA PHYTASE VARIANTS
    3.
    发明申请
    HAFNIA PHYTASE VARIANTS 有权

    公开(公告)号：US20100083392A1

    公开(公告)日：2010-04-01

    申请号：US12568312

    申请日：2009-09-28

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： A01K67/00 ,  C12N9/16 ,  C12P1/00 ,  C12P7/06 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00 ,  C12P21/00 ,  A01H5/00 ,  A23L1/28 ,  A23K1/18 ,  A23K1/16 ,  C12G1/00 ,  C12C11/00 ,  C12C7/00

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Hafnia Phytase Variants
    4.
    发明申请
    Hafnia Phytase Variants 有权
    Hafnia植酸酶变体

    公开(公告)号：US20120225468A1

    公开(公告)日：2012-09-06

    申请号：US13472822

    申请日：2012-05-16

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： C12N9/16

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Hafnia phytase variants
    5.
    发明授权
    Hafnia phytase variants 有权
    Hafnia植酸酶变体

    公开(公告)号：US08206962B2

    公开(公告)日：2012-06-26

    申请号：US12568312

    申请日：2009-09-28

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： C12N9/14 ,  C12Q1/34 ,  C07H21/04

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Hafnia phytase variants
    6.
    发明授权
    Hafnia phytase variants 有权
    Hafnia植酸酶变体

    公开(公告)号：US08507240B2

    公开(公告)日：2013-08-13

    申请号：US13472822

    申请日：2012-05-16

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Lars Kobberoee Skov ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Lars Kobberoee Skov ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Jesper Vind

    IPC分类号： C12N9/16 ,  A23K1/165 ,  A23L1/105 ,  A23L1/20

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。