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    DNA construct encoding the YAP3 signal peptide
    2.
    发明授权
    DNA construct encoding the YAP3 signal peptide 失效
    编码YAP3信号肽的DNA构建体

    公开(公告)号：US5726038A

    公开(公告)日：1998-03-10

    申请号：US446646

    申请日：1995-05-25

    申请人： Lars Christiansen ,  Jens Gunner Litske Petersen

    发明人： Lars Christiansen ,  Jens Gunner Litske Petersen

    IPC分类号： C12N1/19 ,  C07K14/81 ,  C12N9/60 ,  C12N15/09 ,  C12N15/62 ,  C12N15/81 ,  C12P21/02 ,  C12R1/865 ,  C12N15/00

    CPC分类号： C07K14/8103 ,  C12N15/625 ,  C12N15/81 ,  C12N9/60 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/036

    摘要： A DNA construct comprising the following sequence: 5'-P-SP-(LP).sub.n -PS-HP-3' wherein P is a promoter sequence, SP is a DNA sequence encoding the yeast aspartic protease 3 (YAP3) signal peptide, LP is a DNA sequence encoding a leader peptide, n is 0 or 1, PS is a DNA sequence encoding a peptide defining a yeast processing site, and HP is a DNA sequence encoding a polypeptide which is heterologous to a selected host organism. The YAP3 signal peptide provides efficient secretion of heterologous proteins in yeast.

    摘要翻译： PCT No.PCT / DK94 / 00281 Sec。 371日期：1995年5月25日 102（e）日期1995年5月25日PCT提交1994年7月8日PCT公布。 第WO95 / 02059号公报 日期1995年1月19日包含以下序列的DNA构建体：5'-P-SP-（LP）n-PS-HP-3'，其中P是启动子序列，SP是编码酵母天冬氨酸蛋白酶3的DNA序列 YAP3）信号肽，LP是编码前导肽的DNA序列，n是0或1，PS是编码限定酵母加工位点的肽的DNA序列，HP是编码与选择的异源的多肽的DNA序列 宿主生物。 YAP3信号肽在酵母中提供异源蛋白质的高效分泌。

    Cellulase variants
    3.
    发明授权
    Cellulase variants 有权
    纤维素酶变体

    公开(公告)号：US08017372B2

    公开(公告)日：2011-09-13

    申请号：US11830063

    申请日：2007-07-30

    申请人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen, legal representative ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schulein ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42 ,  C12N15/00 ,  C12P21/04 ,  C07H21/04

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Cellulase Variants
    4.
    发明申请
    Cellulase Variants 有权
    纤维素酶变体

    公开(公告)号：US20080206836A1

    公开(公告)日：2008-08-28

    申请号：US11830063

    申请日：2007-07-30

    申请人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Cellulase Variants
    5.
    发明申请
    Cellulase Variants 审中-公开
    纤维素酶变体

    公开(公告)号：US20120289450A1

    公开(公告)日：2012-11-15

    申请号：US13471757

    申请日：2012-05-15

    申请人： Kim Vilbour Andersen ,  Martin Schülein ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schülein ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42 ,  C11D3/386

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to cellulase variants, i.e., endo-beta-1,4-glucanase variants, derived from a parental cellulase, i.e., endo-beta-1,4-glucanase, by substitution, insertion and/or deletion, which variant has a catalytic core domain, in which the variant at position 5 holds an alanine residue (A), a serine residue (S), or a threonine residue (T); at position 8 holds a phenylalanine residue (F), or a tyrosine residue (Y); at position 9 holds a phenylalanine residue (F), a tryptophan residue (W), or a tyrosine residue (Y); at position 10 holds an aspartic acid residue (D); and at position 121 holds an aspartic acid residue (D).

    摘要翻译： 本发明涉及通过取代，插入和/或缺失衍生自亲代纤维素酶（即内切-β-1,4-葡聚糖酶）的纤维素酶变体，即内切-β-1,4-葡聚糖酶变体，该变体 具有催化核心结构域，其中第5位的变体含有丙氨酸残基（A），丝氨酸残基（S）或苏氨酸残基（T）; 在8位保持苯丙氨酸残基（F）或酪氨酸残基（Y）; 位置9含有苯丙氨酸残基（F），色氨酸残基（W）或酪氨酸残基（Y）; 在10位保持天冬氨酸残基（D）; 并且在位置121处保持天冬氨酸残基（D）。

    Synthetic yeast leader peptides
    6.
    发明授权
    Synthetic yeast leader peptides 失效
    合成酵母前导肽

    公开(公告)号：US5316923A

    公开(公告)日：1994-05-31

    申请号：US973483

    申请日：1992-11-09

    申请人： Lars Christiansen

    发明人： Lars Christiansen

    IPC分类号： C12N15/09 ,  C07K14/00 ,  C07K14/395 ,  C07K14/575 ,  C07K14/62 ,  C12N1/19 ,  C12N15/62 ,  C12N15/81 ,  C12P21/02 ,  C12R1/865 ,  C07K7/10

    CPC分类号： C07K14/62 ,  C12N15/625 ,  C12N15/815 ,  C07K2319/036 ,  C07K2319/75

    摘要： Synthetic leaders for effective secretion of proteins in yeast are provided. Also provided are replicable yeast vectors containing a DNA-sequence encoding the synthetic leader positioned upstream to a DNA-sequence encoding the desired product and operably connected with promoter and signal sequences. There are also provided yeast strains transformed with such vectors and a method for producing proteins by means of the transformed yeast strains.

    摘要翻译： 提供了在酵母中有效分泌蛋白质的合成领导者。 还提供了可复制的酵母载体，其含有编码合成前导序列的DNA序列，所述DNA序列位于编码所需产物的DNA序列的上游，并与启动子和信号序列可操作地连接。 还提供了用这种载体转化的酵母菌株和通过转化的酵母菌株产生蛋白质的方法。

    CELLULASE VARIANTS
    8.
    发明申请
    CELLULASE VARIANTS 审中-公开
    纤维素酶变体

    公开(公告)号：US20110250674A1

    公开(公告)日：2011-10-13

    申请号：US13162636

    申请日：2011-06-17

    申请人： Kim Vilbour Andersen ,  Martin Schülein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schülein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Cellulase variants
    9.
    发明申请
    Cellulase variants 审中-公开
    纤维素酶变体

    公开(公告)号：US20050009166A1

    公开(公告)日：2005-01-13

    申请号：US10919195

    申请日：2004-08-16

    申请人： Kim Andersen ,  Martin Schulein ,  Hanne Dela ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Andersen ,  Martin Schulein ,  Hanne Dela ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/15 ,  C12N1/21 ,  C12N9/42 ,  C12R1/645 ,  C12S11/00 ,  C12S99/00 ,  D06L4/40 ,  D06M16/00 ,  D21B1/02 ,  D21C5/02 ,  D21H21/24 ,  C07H21/04 ,  C12N15/74

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。