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公开(公告)号：US08298778B2
公开(公告)日：2012-10-30
申请号：US12866793
申请日：2009-02-06
IPC分类号： C07K1/00 , C07K2/00 , G01N33/53 , G01N33/569
CPC分类号： C07K16/1242 , C07K14/195 , C07K2317/23 , G01N33/56911 , G01N2469/20 , G01N2800/14
摘要： A method and a kit for detecting an antibody to Avibacterium paragallinarum are provided. A method for detecting an antibody to Avibacterium paragallinarum which comprises detecting an antibody induced by an outer-membrane protein of Avibacterium paragallinarum serotype A and/or serotype C by ELISA with a solid phase to which a peptide consisting of an amino acid sequence of non-homologous region of said outer-membrane protein or a portion thereof is immobilized, and a detection kit used for said method.
摘要翻译： 提供了用于检测副杆菌的抗体的方法和试剂盒。 一种检测副杆菌的抗体的方法，其特征在于，检测由Pariballinarum血清型A和/或血清型C的外膜蛋白诱导的抗体，所述抗体通过ELISA与固相相结合，所述固相由非氨基酸序列的氨基酸序列组成， 所述外膜蛋白或其部分的同源区域被固定化，以及用于所述方法的检测试剂盒。
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公开(公告)号：US20110201034A1
公开(公告)日：2011-08-18
申请号：US12866793
申请日：2009-02-06
IPC分类号： G01N33/566 , C07K7/06 , C07K7/08
CPC分类号： C07K16/1242 , C07K14/195 , C07K2317/23 , G01N33/56911 , G01N2469/20 , G01N2800/14
摘要： A method and a kit for detecting an antibody to Avibacterium paragallinarum are provided. A method for detecting an antibody to Avibacterium paragallinarum which comprises detecting an antibody induced by an outer-membrane protein of Avibacterium paragallinarum serotype A and/or serotype C by ELISA with a solid phase to which a peptide consisting of an amino acid sequence of non-homologous region of said outer-membrane protein or a portion thereof is immobilized, and a detection kit used for said method.
摘要翻译： 提供了用于检测副杆菌的抗体的方法和试剂盒。 一种检测副杆菌的抗体的方法，其特征在于，检测由Pariballinarum血清型A和/或血清型C的外膜蛋白诱导的抗体，所述抗体通过ELISA与固相相结合，所述固相由非氨基酸序列的氨基酸序列组成， 所述外膜蛋白或其部分的同源区域被固定化，以及用于所述方法的检测试剂盒。
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3.发明授权Process for preparing variant of Erysipelothrix rhusiopathiae surface protective antigen in E. coli 有权在大肠杆菌中制备欧洲白喉病毒性表皮保护性抗原变异体的方法公开(公告)号：US08277820B2
公开(公告)日：2012-10-02
申请号：US10590896
申请日：2005-02-08
CPC分类号： C07K14/195 , A61K38/00 , A61K39/00
摘要： A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
摘要翻译： 提供了Erysillolothrix rhasiopathiae表面保护性抗原SpaA蛋白或SpaA（＆Dgr。SpaA）的缩写形式的一种变体，其中一部分SpaA蛋白被缺失以防止欧莱氏杆菌性鼻病毒感染及其制备方法。 在SpaA或＆Dgr; SpaA蛋白的氨基酸序列中的特定位点引入氨基酸取代提供了SPAA或＆Dgr; SpaA蛋白的变体，其是免疫原性的并且在大肠杆菌中以包涵体表达。 本发明的SpaA或＆Dgr; SpaA蛋白质的变体可以容易地回收和纯化，因为其在大肠杆菌中作为包涵体表达。
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4.发明授权Process for preparing variant of Erysipelothrix rhusiopathiae surface protective antigen in E. coli 有权在大肠杆菌中制备欧洲白喉病毒性表皮保护性抗原变异体的方法公开(公告)号：US08361749B2
公开(公告)日：2013-01-29
申请号：US12949606
申请日：2010-11-18
CPC分类号： C07K14/195 , A61K38/00 , A61K39/00
摘要： A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
摘要翻译： 提供了Erysillolothrix rhasiopathiae表面保护性抗原SpaA蛋白或SpaA（＆Dgr。SpaA）的缩写形式的一种变体，其中一部分SpaA蛋白被缺失以防止欧莱氏杆菌性鼻病毒感染及其制备方法。 在SpaA或＆Dgr; SpaA蛋白的氨基酸序列中的特定位点引入氨基酸取代提供了SPAA或＆Dgr; SpaA蛋白的变体，其是免疫原性的并且在大肠杆菌中以包涵体表达。 本发明的SpaA或＆Dgr; SpaA蛋白质的变体可以容易地回收和纯化，因为其在大肠杆菌中作为包涵体表达。
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5.发明申请公开(公告)号：US20080286309A1
公开(公告)日：2008-11-20
申请号：US10590896
申请日：2005-02-08
IPC分类号： A61K39/02 , C12P21/00 , C12N15/31 , C07K14/195
CPC分类号： C07K14/195 , A61K38/00 , A61K39/00
摘要： A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
摘要翻译： 提供了Erysillolothrix rhusiopathiae表面保护性抗原SpaA蛋白质或缩短形式的SpaA（DeltaSpaA）的变体，其中一部分SpaA蛋白质被缺失以防止欧洲白喉病毒性鼻炎病毒感染及其制备方法。 在SpaA或DeltaSpaA蛋白的氨基酸序列中的特定位点处引入氨基酸取代提供了SPAA或DeltaSpaA蛋白的变体，其是免疫原性的并且在大肠杆菌中作为包涵体表达。 本发明的SpaA或DeltaSpaA蛋白的变体可以容易地被回收和纯化，因为它作为包涵体在大肠杆菌中表达。
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6.发明申请PROCESS FOR PREPARING VARIANT OF ERYSIPELOTHRIX RHUSIOPATHIAE SURFACE PROTECTIVE ANTIGEN IN E. COLI 有权在大肠杆菌中制备抗坏血酸表面抗原的变体的方法公开(公告)号：US20110059021A1
公开(公告)日：2011-03-10
申请号：US12949606
申请日：2010-11-18
CPC分类号： C07K14/195 , A61K38/00 , A61K39/00
摘要： A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
摘要翻译： 提供了Erysillolothrix rhasiopathiae表面保护性抗原SpaA蛋白或SpaA（＆Dgr。SpaA）的缩写形式的一种变体，其中一部分SpaA蛋白被缺失以防止欧莱氏杆菌性鼻病毒感染及其制备方法。 在SpaA或＆Dgr; SpaA蛋白的氨基酸序列中的特定位点引入氨基酸取代提供了SPAA或＆Dgr; SpaA蛋白的变体，其是免疫原性的并且在大肠杆菌中以包涵体表达。 本发明的SpaA或＆Dgr; SpaA蛋白质的变体可以容易地回收和纯化，因为其在大肠杆菌中作为包涵体表达。
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7.发明申请RECOMBINANT AVIAN INFECTIOUS CORYZA VACCINE AND PROCESS FOR PREPARING SAME 审中-公开重组AVIAN感染性CORYZA VACCINE及其制备方法公开(公告)号：US20130183330A1
公开(公告)日：2013-07-18
申请号：US13616255
申请日：2012-09-14
IPC分类号： A61K39/02
CPC分类号： A61K39/02 , A61K39/102 , C07K14/465
摘要： A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructing E. coli that may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein of Avibacterium paragarinarum serotype A and serotype C, step of culturing said E. coli and colleting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.
摘要翻译： 提供了重组禽传染性鼻炎疫苗及其制备方法。 一种制备重组禽传染性鼻炎疫苗的方法，其包括构建可以产生作为包涵体的大肠杆菌的步骤，所述融合肽由衍生自副杆菌素A型血清型A和血清型C的外膜蛋白的肽组成，培养所述 大肠杆菌和来自培养物的收集和纯化包涵体，以及制备包含所述纯化的包涵体的制剂的步骤和包含作为活性成分的融合肽的禽传染性疫苗疫苗的步骤。 可以在包含融合肽的各个肽之间插入接头序列。 对于源自血清型A和C的肽，可以使用负责防止感染的区域2或其附近的氨基酸序列区域。
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8.发明申请RECOMBINANT AVIAN INFECTIOUS CORYZA VACCINE AND PROCESS FOR PREPARING SAME 有权重组AVIAN感染性CORYZA VACCINE及其制备方法公开(公告)号：US20120003257A1
公开(公告)日：2012-01-05
申请号：US13142178
申请日：2009-12-24
CPC分类号： A61K39/02 , A61K39/102 , C07K14/465
摘要： A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructing E. coli that may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein of Avibacterium paragarinarum serotype A and serotype C, step of culturing said E. coli and colleting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.
摘要翻译： 提供了重组禽传染性鼻炎疫苗及其制备方法。 一种制备重组禽传染性鼻炎疫苗的方法，其包括构建可以产生作为包涵体的大肠杆菌的步骤，所述融合肽由衍生自副杆菌素A型血清型A和血清型C的外膜蛋白的肽组成，培养所述 大肠杆菌和来自培养物的收集和纯化包涵体，以及制备包含所述纯化的包涵体的制剂的步骤和包含作为活性成分的融合肽的禽传染性疫苗疫苗的步骤。 可以在包含融合肽的各个肽之间插入接头序列。 对于源自血清型A和C的肽，可以使用负责防止感染的区域2或其附近的氨基酸序列区域。
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9.发明授权公开(公告)号：US08647634B2
公开(公告)日：2014-02-11
申请号：US13142178
申请日：2009-12-24
CPC分类号： A61K39/02 , A61K39/102 , C07K14/465
摘要： A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructing E. coli that may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein of Avibacterium paragarinarum serotype A and serotype C, step of culturing said E. coli and collecting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.
摘要翻译： 提供了重组禽传染性鼻炎疫苗及其制备方法。 一种制备重组禽传染性鼻炎疫苗的方法，其包括构建可以产生作为包涵体的大肠杆菌的步骤，所述融合肽由衍生自副杆菌素A型血清型A和血清型C的外膜蛋白的肽组成，培养所述 大肠杆菌，从培养物收集和纯化包涵体，以及制备包含所述纯化的包涵体的制剂的步骤和包含作为活性成分的融合肽的禽传染性疫苗疫苗。 可以在包含融合肽的各个肽之间插入接头序列。 对于源自血清型A和C的肽，可以使用负责防止感染的区域2或其附近的氨基酸序列区域。
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公开(公告)号：US20120321237A1
公开(公告)日：2012-12-20
申请号：US13578928
申请日：2011-02-22
申请人： Isao Usuki , Hiroshi Ueno , Hirosato Nakanishi , Masashi Sakaguchi , Kazuki Hamada , Hironori Hiraoka
发明人： Isao Usuki , Hiroshi Ueno , Hirosato Nakanishi , Masashi Sakaguchi , Kazuki Hamada , Hironori Hiraoka
IPC分类号： F16C33/46
CPC分类号： F16C33/6614 , F16C19/364 , F16C33/4635 , F16C33/4676 , F16C2240/46 , F16C2300/02
摘要： A conical roller cage that implements a conical roller bearing that is able to suppress torque and that is able to smoothly supply lubricant to a raceway surface includes: a small-diameter annular portion, a large-diameter annular portion having a bore diameter larger than a bore diameter of the small-diameter annular portion, pillar portions that couple the small-diameter annular portion to the large-diameter annular portion and that are located at intervals from each other in a circumferential direction, roller partition wall portions each radially extending from a radial end face of a corresponding one of the pillar portions, and canopy portions each protruding from a radial end portion of a corresponding one the roller partition wall portions, at an opposite side to a side of the pillar portion, toward both sides in the circumferential direction.
摘要翻译： 实现能够抑制扭矩并且能够平稳地将润滑剂供给到滚道表面的圆锥滚子轴承的圆锥滚子保持架包括：小直径环形部分，具有大于 所述小直径环状部的孔径，将所述小径环状部与所述大径环状部连接并且在周向上彼此间隔配置的柱部，从所述小直径环状部沿径向延伸的辊隔壁壁部 相对应的一个支柱部的径向端面和从相对应的一个的分隔壁部的与该柱部的一侧相反侧的径向端部朝向圆周方向的两侧突出的顶盖部 方向。
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