公开(公告)号：US20180271502A1

公开(公告)日：2018-09-27

申请号：US15762143

申请日：2016-09-22

Applicant: University Health Network

Inventor： Arash Zarrine-Afsar ,  David A. Jaffray ,  Alessandra Tata ,  Michael Woolman ,  Alexander Vitkin

IPC: A61B10/02 ,  H01J49/00 ,  H01J49/04 ,  A61B34/10 ,  A61B18/12 ,  A61B18/20 ,  A61B5/00 ,  A61B5/055 ,  A61B6/03 ,  A61B8/08

CPC classification number: A61B10/0266 ,  A61B5/0095 ,  A61B5/055 ,  A61B6/032 ,  A61B6/037 ,  A61B8/085 ,  A61B10/0233 ,  A61B18/12 ,  A61B18/20 ,  A61B34/10 ,  A61B2018/00577 ,  A61B2018/00595 ,  A61B2018/00904 ,  A61B2034/107 ,  A61B2034/2072 ,  G01N21/21 ,  H01J49/0004 ,  H01J49/0031 ,  H01J49/04

Abstract: Various embodiments are described herein for a system and a method for obtaining samples of tissue for analysis by mass spectrometry. A region of interest can be identified in tissue using image data from a first imaging modality that is other than mass spectrometry. At least one tissue sample can be acquired using a tissue sampler from a sampling location related to the region of interest. Mass spectrum data can be generated for the acquired tissue samples using a mass spectrometer. In some embodiments. polarimetry may be used on a tissue slice, mass spectrometry may be performed on the same tissue slice and then H&E imaging may be performed on the same tissue slice.

公开(公告)号：US10811242B2

公开(公告)日：2020-10-20

申请号：US16308749

申请日：2017-06-09

Applicant: University Health Network ,  Unity Health Toronto

Inventor： Arash Zarrine-Afsar ,  Howard Joeseph Ginsberg ,  Michael Woolman

IPC: H01J49/04 ,  H01J49/00

Abstract: Methods and systems are provided for ionizing molecules for the purpose of analysis by mass spectrometry, in which gaseous material from a sample substrate is generated using laser desorption. The laser is provided having a pulse range of about 1-1000 picoseconds to produce the gaseous material. The gaseous material is heated to generate ions from the molecules present in the gaseous material where the amount of heat that is applied is in the temperature range of 45° C. to 250° C. and the applied heat results in soft ionization of the molecules. The ionized molecules are transported to a mass spectrometer for analysis.

公开(公告)号：US11266383B2

公开(公告)日：2022-03-08

申请号：US15762143

申请日：2016-09-22

Applicant: University Health Network

Inventor： Arash Zarrine-Afsar ,  David A. Jaffray ,  Alessandra Tata ,  Michael Woolman ,  Alexander Vitkin

IPC: G01N21/00 ,  A61B10/02 ,  A61B18/20 ,  H01J49/04 ,  G01N21/21 ,  A61B34/10 ,  A61B5/00 ,  A61B5/055 ,  A61B6/03 ,  A61B8/08 ,  A61B18/12 ,  H01J49/00 ,  A61B34/20 ,  A61B18/00

Abstract: Various embodiments are described herein for a system and a method for obtaining samples of tissue for analysis by mass spectrometry. A region of interest can be identified in tissue using image data from a first imaging modality that is other than mass spectrometry. At least one tissue sample can be acquired using a tissue sampler from a sampling location related to the region of interest. Mass spectrum data can be generated for the acquired tissue samples using a mass spectrometer. In some embodiments, polarimetry may be used on a tissue slice, mass spectrometry may be performed on the same tissue slice and then H&E imaging may be performed on the same tissue slice.

公开(公告)号：US20200144044A1

公开(公告)日：2020-05-07

申请号：US16308749

申请日：2017-06-09

Applicant: University Health Network ,  Unity Health Toronto

Inventor： Arash Zarrine-Afsar ,  Howard Joeseph Ginsberg ,  Michael Woolman

IPC: H01J49/04 ,  H01J49/00

Abstract: Methods and systems are provided for ionizing molecules for the purpose of analysis by mass spectrometry, in which gaseous material from a sample substrate is generated using laser desorption. The laser is provided having a pulse range of about 1-1000 picoseconds to produce the gaseous material. The gaseous material is heated to generate ions from the molecules present in the gaseous material where the amount of heat that is applied is in the temperature range of 45° C. to 250° C. and the applied heat results in soft ionization of the molecules. The ionized molecules are transported to a mass spectrometer for analysis.