公开(公告)号：US20220371006A1

公开(公告)日：2022-11-24

申请号：US17761138

申请日：2020-10-01

Applicant: University of Washington

Inventor： Paul Yager ,  Steven Bennett ,  Sujatha Kumar ,  Kamal Girish Shah ,  Erin K. Heiniger

IPC: B01L3/00 ,  G01N1/14

Abstract: An apparatus, system, and method for filtering and assaying a fluid sample are described. In an embodiment, the apparatus includes a filtration unit comprising: a filter bracket shaped to removably couple with a fluid sample cup and a vacuum container; and a filter housing cooperatively couplable to the filter bracket and comprising a filter configured to filter fluid passing through the filter bracket; and an assay device shaped to cooperatively couple with the filter housing and comprising a porous matrix positioned to be in fluidic communication with the filter when the filter housing is cooperatively coupled with the assay device.

公开(公告)号：US20170131211A1

公开(公告)日：2017-05-11

申请号：US15348926

申请日：2016-11-10

Applicant: University of Washington

Inventor： Paul Yager ,  Joshua Bishop ,  Joshua Buser ,  Louise Lyth Hansen ,  Erin K. Heiniger ,  Enos Kline ,  Sujatha Kumar

IPC: G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6486 ,  B01L3/00 ,  B01L3/5023 ,  B01L2200/026 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/1827 ,  B01L2400/0406 ,  C12Q1/6816 ,  C12Q1/6844 ,  G01N1/286 ,  G01N21/645 ,  G01N2001/4088

Abstract: The present technology relates generally to systems for disrupting biological samples and associated devices and methods. In some embodiments, the system includes a vessel configured to receive a biological sample and a cap assembly that includes a porous membrane having a receiving region and a detection region. When the cap assembly is detachably coupled to an open end portion of the vessel, the system can be moved between a first orientation and a second orientation. When the system is in the first orientation, the biological sample is not in fluid communication with the receiving region. When the vessel contains is in the second orientation, the biological sample is in fluid communication with the receiving region and wicks through the porous membrane to the detection region.

公开(公告)号：US20220395838A1

公开(公告)日：2022-12-15

申请号：US17838807

申请日：2022-06-13

Applicant: University of Washington

Inventor： Paul Yager ,  Steven Bennett ,  Erin K. Heiniger ,  Sujatha Kumar ,  Kamal Girish Shah

IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/6806

Abstract: Fluidic devices, systems, and methods for analyzing an analyte are described. In an embodiment, the fluidic devices include a housing defining a lysis chamber shaped to receive a biological sample; a lysis buffer storage chamber disposed within the housing and carrying a lysis buffer configured to lyse cells of the biological sample; a cap configured to cooperatively couple to the housing; a compressor configured to compress the lysis buffer storage chamber and expel the lysis buffer from the lysis buffer storage chamber and into the lysis chamber when the cap is uncoupled from the housing; and a porous membrane in selective fluidic communication with the lysis chamber.

公开(公告)号：US20160310942A1

公开(公告)日：2016-10-27

申请号：US15135461

申请日：2016-04-21

Applicant: University of Washington

Inventor： Paul Yager ,  Joshua Buser ,  Samantha Byrnes ,  Erin K. Heiniger ,  Peter C. Kauffman ,  Paula Ladd

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/50273 ,  B01L2300/069 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0683 ,  C12Q1/6844

Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a first porous element having a first pore size and configured to receive a fluid at its proximal portion, and a second porous element having a second pore size greater than the first pore size and configured to receive a fluid at its proximal portion. The first porous element can be positioned across the second porous element such that an overlapping region exists between the porous elements where the porous elements are in fluid communication. Before delivery of the fluid to the second porous element, the fluid pressure at the overlapping region is greater than the capillary pressure of the second porous element such that a fluid delivered to the first porous element wicks through its overlapping portion without wetting the second porous element.

Abstract translation: 本技术涉及用于执行化学过程和相关系统和方法的基于毛细管的装置。 在一个实施例中，例如，装置可以包括具有第一孔径并被配置为在其近端部分处接收流体的第一多孔元件和具有大于第一孔径的第二孔径的第二多孔元件，并且被配置为 在其近端部分接收流体。 第一多孔元件可以跨越第二多孔元件定位，使得在多孔元件处于流体连通的多孔元件之间存在重叠区域。 在将流体输送到第二多孔元件之前，重叠区域处的流体压力大于第二多孔元件的毛细管压力，使得输送到第一多孔元件的流体通过其重叠部分吸入而不润湿第二多孔元件 。