公开(公告)号：US20230272462A1

公开(公告)日：2023-08-31

申请号：US18004489

申请日：2021-07-09

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Enos Kline ,  Michael Roller ,  Robert G. Atkinson ,  Nuttada Panpradist ,  Daniel Leon ,  Ian Hull ,  Qin Wang ,  Barry R. Lutz

IPC: C12Q1/6844 ,  C12Q1/6883 ,  C12N15/63 ,  B01L7/00

CPC classification number: C12Q1/6844 ,  C12Q1/6883 ,  C12N15/63 ,  B01L7/52 ,  C12N2310/122 ,  B01L2300/1822

Abstract: Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucleic acid molecule comprising: a targeting sequence complementary to a target portion of a target nucleic acid sequence; and an adapter sequence; a displacement nucleic acid probe comprising: a fluorophore adapter sequence; and the adapter sequence; and a fluorophore adapter complement nucleic acid molecule complementary to the fluorophore adapter sequence, wherein the fluorophore adapter sequence or the fluorophore adapter complement nucleic acid molecule is coupled to a fluorophore. In an embodiment, the system comprises a thermal subsystem for heating a sample disposed therein, and an optical subsystem for optically excited the sample and detecting light emitted from the sample.

公开(公告)号：US20170131211A1

公开(公告)日：2017-05-11

申请号：US15348926

申请日：2016-11-10

Applicant: University of Washington

Inventor： Paul Yager ,  Joshua Bishop ,  Joshua Buser ,  Louise Lyth Hansen ,  Erin K. Heiniger ,  Enos Kline ,  Sujatha Kumar

IPC: G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6486 ,  B01L3/00 ,  B01L3/5023 ,  B01L2200/026 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/1827 ,  B01L2400/0406 ,  C12Q1/6816 ,  C12Q1/6844 ,  G01N1/286 ,  G01N21/645 ,  G01N2001/4088

Abstract: The present technology relates generally to systems for disrupting biological samples and associated devices and methods. In some embodiments, the system includes a vessel configured to receive a biological sample and a cap assembly that includes a porous membrane having a receiving region and a detection region. When the cap assembly is detachably coupled to an open end portion of the vessel, the system can be moved between a first orientation and a second orientation. When the system is in the first orientation, the biological sample is not in fluid communication with the receiving region. When the vessel contains is in the second orientation, the biological sample is in fluid communication with the receiving region and wicks through the porous membrane to the detection region.