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    AMPLIFICATION AND DETECTION OF NUCLEIC ACIDS
    2.
    发明申请
    AMPLIFICATION AND DETECTION OF NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20190009276A1

    公开(公告)日：2019-01-10

    申请号：US16036430

    申请日：2018-07-16

    Applicant: GENERAL ELECTRIC COMPANY ,  University of Washington

    Inventor： David Roger Moore ,  Matthew Jeremiah Misner ,  Andrew Arthur Paul Burns ,  Joshua Bishop ,  Lisa K. Lafleur ,  Maxwell Wheeler

    IPC: B01L7/00 ,  C12Q1/6844 ,  B01L3/00 ,  C12Q1/6806

    Abstract: The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.

    Proximity-enhanced nucleic acid-amplified protein detection
    3.
    发明申请
    Proximity-enhanced nucleic acid-amplified protein detection 审中-公开

    公开(公告)号：US20180119202A1

    公开(公告)日：2018-05-03

    申请号：US15801223

    申请日：2017-11-01

    Applicant: University of Washington

    Inventor： Paul Yager ,  Joshua Bishop ,  Erin Heiniger

    IPC: C12Q1/68 ,  G01N33/543

    CPC classification number: C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N33/54306 ,  C12Q2565/531 ,  C12Q2563/179

    Abstract: The present technology is related to methods and compositions for detecting, and optionally quantifying, one or more analytes of a sample using nucleic acids. In some embodiments, the methods include generating a complex of a plurality of peptides, an analyte, a first nucleic acid, and a second nucleic acid, each nucleic acid conjugated to a binder peptide. In addition, an immobilizer peptide can be immobilized to a substrate. If the binder peptides are bound to the analyte, the method further includes hybridizing a segment of the first nucleic acid to a segment of the second nucleic acid and amplifying the hybridized nucleic acids to generate a plurality of amplicons. Moreover, the generated amplicons indicate that one or more analytes has been detected. A number of generated amplicons can be analyzed to quantify one or more of the bound analytes.

    Amplification and detection of nucleic acids
    5.
    发明授权
    Amplification and detection of nucleic acids 有权

    公开(公告)号：US11173490B2

    公开(公告)日：2021-11-16

    申请号：US16036430

    申请日：2018-07-16

    Applicant: GENERAL ELECTRIC COMPANY ,  University of Washington

    Inventor： David Roger Moore ,  Matthew Jeremiah Misner ,  Andrew Arthur Paul Burns ,  Joshua Bishop ,  Lisa K. Lafleur ,  Maxwell Wheeler

    IPC: B01L7/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  B01L3/00

    Abstract: The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.

    Amplification and detection of nucleic acids
    7.
    发明授权
    Amplification and detection of nucleic acids 有权

    公开(公告)号：US10040069B2

    公开(公告)日：2018-08-07

    申请号：US14807497

    申请日：2015-07-23

    Applicant: GENERAL ELECTRIC COMPANY ,  University of Washington

    Inventor： David Roger Moore ,  Matthew Jeremiah Misner ,  Andrew Arthur Paul Burns ,  Joshua Bishop ,  Lisa K. Lafleur ,  Maxwell Wheeler

    IPC: C12Q1/68 ,  C12P19/34 ,  B01L7/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  B01L3/00

    Abstract: The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.

    Temperature-actuated valve, fluidic device, and related methods of use
    8.
    发明授权
    Temperature-actuated valve, fluidic device, and related methods of use 有权

    公开(公告)号：US10935149B2

    公开(公告)日：2021-03-02

    申请号：US16354987

    申请日：2019-03-15

    Applicant: University of Washington

    Inventor： Paul Yager ,  Joshua Bishop ,  Michael Purfield

    IPC: F16K17/38 ,  F16K99/00 ,  B01L3/00

    Abstract: Temperature-actuated valves, devices including temperature-actuated valves, and related methods are described. In an embodiment, the temperature-actuated valve includes a heat-shrink film defining a perforation extending at least partially in a first direction. In an embodiment, the temperature-actuated valve is configured to open when a portion of the heat-shrink film including the perforation is heated above a threshold temperature to contract the heat-shrink film along a second direction perpendicular to the first direction to define an aperture, in an open configuration, providing a fluid a path through the heat-shrink film. In an embodiment, the temperature-actuated valve includes a leakage-mitigation feature configured to limit fluid flow through the perforation when the valve is in a closed configuration.

    TEMPERATURE-ACTUATED VALVE, FLUIDIC DEVICE, AND RELATED METHODS OF USE
    9.
    发明申请
    TEMPERATURE-ACTUATED VALVE, FLUIDIC DEVICE, AND RELATED METHODS OF USE 审中-公开

    公开(公告)号：US20190285187A1

    公开(公告)日：2019-09-19

    申请号：US16354987

    申请日：2019-03-15

    Applicant: University of Washington

    Inventor： Paul Yager ,  Joshua Bishop ,  Michael Purfield

    IPC: F16K17/38 ,  F16K99/00

    Abstract: Temperature-actuated valves, devices including temperature-actuated valves, and related methods are described. In an embodiment, the temperature-actuated valve includes a heat-shrink film defining a perforation extending at least partially in a first direction. In an embodiment, the temperature-actuated valve is configured to open when a portion of the heat-shrink film including the perforation is heated above a threshold temperature to contract the heat-shrink film along a second direction perpendicular to the first direction to define an aperture, in an open configuration, providing a fluid a path through the heat-shrink film. In an embodiment, the temperature-actuated valve includes a leakage-mitigation feature configured to limit fluid flow through the perforation when the valve is in a closed configuration.

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