公开(公告)号：US11873347B2

公开(公告)日：2024-01-16

申请号：US16760394

申请日：2018-10-31

申请人： VIB VZW ,  VRIJE UNIVERSITEIT BRUSSEL

发明人： Jan Steyaert ,  Els Pardon ,  Tomasz Uchanski ,  Wim Vranken

IPC分类号： C07K16/46 ,  C07K14/195 ,  G01N23/20 ,  G01N33/68 ,  C07K14/705 ,  C07K14/005 ,  C12N7/00

CPC分类号： C07K16/465 ,  C07K14/005 ,  C07K14/195 ,  C07K14/705 ,  C12N7/00 ,  G01N23/20 ,  G01N33/6845 ,  C07K2299/00 ,  C07K2317/569 ,  C07K2319/03 ,  C12N2795/00022 ,  C12N2795/00023

摘要： The present invention relates to the field of structural biology. More specifically, the present invention relates to novel antigen-binding chimeric proteins, their uses and methods in three-dimensional structural analysis of macromolecules, such as X-ray crystallography and high-resolution Cryo-EM, and their use as a therapeutic, diagnostic, or imaging tool. Even more specifically, the invention relates to a fusion of a scaffold protein and an antigen-binding domain wherein the scaffold protein of said fusion interrupts the Immunoglobulin domain topology to form a rigid chimer.

公开(公告)号：US20240174767A1

公开(公告)日：2024-05-30

申请号：US18516777

申请日：2023-11-21

申请人： VIB VZW ,  Vrije Universiteit Brussel

发明人： Jan Steyaert ,  Els Pardon ,  Tomasz Uchanski ,  Wim Vranken

IPC分类号： C07K16/46 ,  C07K14/005 ,  C07K14/195 ,  C07K14/705 ,  C12N7/00 ,  G01N23/20 ,  G01N33/68

CPC分类号： C07K16/465 ,  C07K14/005 ,  C07K14/195 ,  C07K14/705 ,  C12N7/00 ,  G01N23/20 ,  G01N33/6845 ,  C07K2299/00 ,  C07K2317/569 ,  C07K2319/03 ,  C07K2319/30 ,  C12N2795/00022 ,  C12N2795/00023

摘要： The present invention relates to the field of structural biology. More specifically, the present invention relates to novel antigen-binding chimeric proteins, their uses and methods in three-dimensional structural analysis of macromolecules, such as X-ray crystallography and high-resolution Cryo-EM, and their use as a therapeutic, diagnostic, or imaging tool. Even more specifically, the invention relates to a fusion of a scaffold protein and an antigen-binding domain wherein the scaffold protein of said fusion interrupts the Immunoglobulin domain topology to form a rigid chimer.

公开(公告)号：US20210206880A1

公开(公告)日：2021-07-08

申请号：US16760394

申请日：2018-10-31

申请人： VIB VZW ,  VRIJE UNIVERSITEIT BRUSSEL

发明人： Jan Steyaert ,  Els Pardon ,  Tomasz Uchanski ,  Wim Vranken

IPC分类号： C07K16/46 ,  C07K14/195 ,  G01N33/68 ,  C07K14/005 ,  C12N7/00 ,  G01N23/20

摘要： The present invention relates to the field of structural biology. More specifically, the present invention relates to novel antigen-binding chimeric proteins, their uses and methods in three-dimensional structural analysis of macromolecules, such as X-ray crystallography and high-resolution Cryo-EM, and their use as a therapeutic, diagnostic, or imaging tool. Even more specifically, the invention relates to a fusion of a scaffold protein and an antigen-binding domain wherein the scaffold protein of said fusion interrupts the Immunoglobulin domain topology to form a rigid chimer.

公开(公告)号：US20220073574A1

公开(公告)日：2022-03-10

申请号：US17415461

申请日：2019-12-20

申请人： VIB VZW ,  VRIJE UNIVERSITEIT BRUSSEL

发明人： Jan Steyaert ,  Els Pardon ,  Wim Vranken

IPC分类号： C07K14/435 ,  G16B15/20

摘要： The present invention relates to the field of structural biology and drug discovery. More specifically, the present invention relates to novel fusion proteins, their uses and methods in three-dimensional structural analysis of macromolecules, such as X-ray crystallography and high-resolution Cryo-EM, and their use in structure-based drug design and screening, and as pharmacological tools. Even more specifically, the invention relates to a functional fusion of a toxin and a scaffold protein wherein the folded scaffold protein interrupts the topology of the toxin by insertion in an exposed β-turn of a β-strand-containing domain of said toxin to form a rigid fusion protein that retains its high affinity target binding capacity.

公开(公告)号：US20220064245A1

公开(公告)日：2022-03-03

申请号：US17415355

申请日：2019-12-20

申请人： VIB VZW ,  VRIJE UNIVERSITEIT BRUSSEL ,  LUXEMBOURG INSTITUTE OF HEALTH

发明人： Jan Steyaert ,  Els Pardon ,  Alexandre Wohlkönig ,  Valentina Kalichuk ,  Wim Vranken ,  Tomasz Uchanski ,  Andy Chevigné ,  Martyna Szpakowska

IPC分类号： C07K14/52

摘要： The present invention relates to the field of structural biology. More specifically, the present invention relates to novel fusion proteins, their uses and methods in three-dimensional structural analysis of macromolecules, such as X-ray crystallography and high-resolution Cryo-EM, and their use in structure-based drug design and screening. Even more specifically, the invention relates to a functional fusion protein of a cytokine and a scaffold protein wherein the scaffold is a folded protein that interrupts the topology of the cytokine to form a rigid fusion protein that retains its receptor binding and activation capacity. More specifically, chemokine- and interleukin-based functional fusion proteins, and their production and uses, are disclosed herein.