摘要:
The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries. In particular, the method employs preparation of a DNA molecule by digesting the DNA molecule with at least one methylation-sensitive restriction enzyme; incorporating a nucleic acid molecule into at least some of the digested DNA molecules by either (1) incorporating at least one primer from a plurality of primers that have a 5′ constant sequence and a 3′ variable sequence, wherein the primers are substantially non-self-complementary and substantially non-complementary to other primers in the plurality; or (2) incorporating an oligonucleotide having an inverted repeat and a loop under conditions wherein the oligonucleotide becomes blunt-end ligated to one strand of the digested DNA molecule, followed by polymerization from a 3′ hydroxyl group present in a nick in the oligonucleotide-linked molecule; and amplifying one or more of the DNA molecules.
摘要:
The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
摘要:
The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
摘要:
The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
摘要:
The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries. In several aspects of the present invention, there are methods based on methylation-dependent enrichment or depletion of genomic DNA isolated from cellular and cell-free sources. In additional embodiments, there are methods and compositions for single-step high throughput preparations of Methylome libraries.
摘要:
The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
摘要:
A dynamic damper in which the inner diameter (D2) of a first hole part formed at the axial one end part of a body part overlapped with a weight part in the radial direction is set smaller than the inner diameter (D3) of a second hole part formed at the axial other end part of the body part not overlapped with the weight part in the radial direction and tightened with a band member (D3>D2). The outer diameter (D1) of a drive shaft to be press-fitted into the first and second hole parts is set larger than the inner diameter (D2) of the first hole part and the inner diameter (D3) of the second hole part (D1>D3>D2).
摘要:
A spread spectrum receiving device is disclosed, in which data demodulation is effected in such a way that a received signal is divided into two parts, from which a COS PN code chip signal and a SIN PN code chip signal are obtained; that these chip signals are A/D-converted by means of A/D converters and then given to digital correlators; and that correlation outputs thus obtained are combined to synthesize a final correlation output. Further there are disposed a plurality of steering gates on the input side of the A/D converters. The a/D conversion can be effected in the neighborhood of a point, where the level of PN code chip waveforms is stable, owing to the fact that the steering gates are controlled by control signals obtained by processing the correlation outputs or output of the A/D conversion. In this way it is made unnecessary to raise the sampling frequency for the A/D conversion and to increase the number of stages of shift registers in the digital correlators.
摘要:
A GOLD code generating device in an SSC device is disclosed, which is so constructed that, in the case where two kinds of GOLD codes are used in the SSC device by the CSK system, etc., in order to generate a GOLD code by means of two m sequence codes, a first GOLD code is generated by using an m sequence code generated by one of the m sequence code generators and another m sequence code generated by the other m sequence code generator, while a second GOLD code is generated by using an m sequence code obtained by delaying the m sequence code generated by the other m sequence code generator by a predetermined number of chips.
摘要:
A pseudo noise code generator is disclosed, in which the shift register value of the pseudo noise code generator corresponding to the generation timing of a bit pulse or a sampling pulse is latched and the output of the latch is compared with the shift register value of the generator, the bit pulse or the sampling pulse being obtained, when they are in accordance with each other.