公开(公告)号：US08841096B2

公开(公告)日：2014-09-23

申请号：US13143354

申请日：2010-01-13

申请人： Volker Sieber ,  Katrin Grammann ,  Broder Ruehmann ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Kai Doderer ,  Claudia Rollmann ,  Arne Skerra ,  Christian Rausch ,  Alexandra Lerchner

发明人： Volker Sieber ,  Katrin Grammann ,  Broder Ruehmann ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Kai Doderer ,  Claudia Rollmann ,  Arne Skerra ,  Christian Rausch ,  Alexandra Lerchner

IPC分类号： C12P17/18 ,  C12N9/06

CPC分类号： C07D493/04 ,  C12P13/001 ,  C12P17/181

摘要： The invention relates to a method for the amination of at least one keto group in a multicyclic ring system comprising at least one keto group into an amino group, using at least one enzyme E having transaminase activity.

摘要翻译： 本发明涉及使用至少一种具有转氨酶活性的酶E，将包含至少一个酮基的多环系统中的至少一个酮基胺化成氨基的方法。

公开(公告)号：US20120041216A1

公开(公告)日：2012-02-16

申请号：US13143354

申请日：2010-01-13

申请人： Volker Sieber ,  Katrin Grammann ,  Broder Ruehmann ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Kai Doderer ,  Claudia Rollmann ,  Arne Skerra ,  Christian Rausch ,  Alexandra Lerchner

发明人： Volker Sieber ,  Katrin Grammann ,  Broder Ruehmann ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Kai Doderer ,  Claudia Rollmann ,  Arne Skerra ,  Christian Rausch ,  Alexandra Lerchner

IPC分类号： C07D493/04 ,  C12P17/18

CPC分类号： C07D493/04 ,  C12P13/001 ,  C12P17/181

摘要： The invention relates to a method for the amination of at least one keto group in a multicyclic ring system comprising at least one keto group into an amino group, using at least one enzyme E having transaminase activity.

摘要翻译： 本发明涉及使用至少一种具有转氨酶活性的酶E，将包含至少一个酮基的多环系统中的至少一个酮基胺化成氨基的方法。

公开(公告)号：US09580732B2

公开(公告)日：2017-02-28

申请号：US14233505

申请日：2012-07-17

申请人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

发明人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  Y02P20/582

摘要： A method including the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, where R1 is —OH, —SH, —NH2 or —COOR2; x is at least 3; and R2 is H, alkyl or aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, where the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme. A whole cell catalyst for carrying out the method. The use of such a whole cell catalyst for oxidizing a primary alcohol.

摘要翻译： 一种方法，包括步骤a）提供式HO-（CH 2）x -R 1的伯醇，其中R 1是-OH，-SH，-NH 2或-COOR 2; x至少为3; 并且R2是H，烷基或芳基，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化伯醇，和c）使步骤a）的氧化产物与转氨酶接触，其中NAD ）+ - 醇脱氢酶和/或转氨酶是重组或分离的酶。 用于实施该方法的全细胞催化剂。 使用这种全细胞催化剂来氧化伯醇。

公开(公告)号：US20140308717A1

公开(公告)日：2014-10-16

申请号：US14237121

申请日：2012-07-27

申请人： Thomas Haas ,  Markus Poetter ,  Jan Christoph Pfeffer ,  Wolfgang Kroutil ,  Arne Skerra ,  Alexandra Lerchner ,  Katharina Christin Tauber ,  Johann H. Sattler ,  Steffen Schaffer

发明人： Thomas Haas ,  Markus Poetter ,  Jan Christoph Pfeffer ,  Wolfgang Kroutil ,  Arne Skerra ,  Alexandra Lerchner ,  Katharina Christin Tauber ,  Johann H. Sattler ,  Steffen Schaffer

IPC分类号： C12P13/00 ,  C12P17/04 ,  C12N15/63

CPC分类号： C12P13/001 ,  C12N9/0006 ,  C12N9/1096 ,  C12N15/63 ,  C12P13/005 ,  C12P13/04 ,  C12P17/04 ,  C12Y101/01001 ,  C12Y206/01 ,  C12Y206/01018

摘要： The present invention relates to a method comprising the steps a) providing a secondary alcohol, b) oxidizing the secondary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-dependent alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a secondary alcohol.

摘要翻译： 本发明涉及包括以下步骤的方法：a）提供仲醇，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化仲醇，和c）将步骤a）的氧化产物与 转氨酶，其中NAD（P）+依赖性醇脱氢酶和/或转氨酶是重组或分离的酶，用于实施该方法的全细胞催化剂，以及使用这种全细胞催化剂来氧化二级 醇。

公开(公告)号：US20140242646A1

公开(公告)日：2014-08-28

申请号：US14233505

申请日：2012-07-17

申请人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

发明人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  Y02P20/582

摘要： The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, wherein R1 is selected from the group consisting of —OH, —SH, —NH2 and —COOR2, x is at least 3 and R2 is selected from the group consisting of H, alkyl and aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a primary alcohol.

摘要翻译： 本发明涉及包括以下步骤的方法：a）提供式HO-（CH 2）x -R 1的伯醇，其中R 1选自-OH，-SH，-NH 2和-COOR 2，x 至少3，R2选自H，烷基和芳基，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化伯醇，和c）使步骤a的氧化产物 ）与转氨酶，其中NAD（P）+ - 醇脱氢酶和/或转氨酶是重组或分离的酶，用于实施该方法的全细胞催化剂，以及使用这种全细胞催化剂进行氧化 一个小酒精。

公开(公告)号：US20150099282A1

公开(公告)日：2015-04-09

申请号：US14395666

申请日：2013-04-16

申请人： Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Alexandra Lerchner ,  Alexander Jarasch

发明人： Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Alexandra Lerchner ,  Alexander Jarasch

IPC分类号： C12P13/06 ,  C12N9/06

CPC分类号： C12P13/06 ,  C12N9/0016 ,  C12P7/24 ,  C12Y104/01001 ,  Y02E50/17

摘要： The present invention relates to a polypeptide comprising the amino acid sequence of the Bacillus subtilis alanine dehydrogenase or a variant thereof, where Leu197 or an amino acid which is located at a homologous position in the amino acid sequence is exchanged for an amino acid with a positively charged side chain, to a nucleic acid molecule encoding such a polypeptide, and to a process for the production of alanine or a compound generated with consumption of alanine, comprising the step of reacting pyruvate with ammonium and NADPH to give alanine by bringing the pyruvate into contact with the polypeptide according to the invention or with the cell according to the invention.

摘要翻译： 本发明涉及包含枯草芽孢杆菌丙氨酸脱氢酶或其变体的氨基酸序列的多肽，其中Leu197或位于氨基酸序列中的同源位置的氨基酸被正交地交换为氨基酸 电荷侧链，编码这种多肽的核酸分子，以及生产丙氨酸或消耗丙氨酸生成的化合物的方法，包括使丙酮酸与铵和NADPH反应的步骤，通过将丙酮酸引入到丙氨酸中而得到丙氨酸 与根据本发明的多肽或根据本发明的细胞接触。

公开(公告)号：US20150111254A1

公开(公告)日：2015-04-23

申请号：US14390133

申请日：2013-03-25

申请人： Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Markus Pötter ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Jasmin Corthals ,  Eva-Maria Eckl ,  Silvana Roeder ,  Wolfgang Kroutil ,  Arne Skerra

发明人： Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Markus Pötter ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Jasmin Corthals ,  Eva-Maria Eckl ,  Silvana Roeder ,  Wolfgang Kroutil ,  Arne Skerra

IPC分类号： C12P13/06

CPC分类号： C12P13/06 ,  C12P13/005

摘要： The problem addressed by the invention is solved in a first aspect by a method for producing alanine or a compound produced using alanine, having the steps of (a) providing a cell which expresses a recombinant alanine dehydrogenase or a variant thereof, (b) cultivating the cell under aerobic conditions in the presence of an inorganic nitrogen source in an aqueous phase, and (c) bringing the cell into contact with an organic phase, said cell being a prokaryotic or a lower eukaryotic cell.

摘要翻译： 本发明解决的问题在第一方面通过一种制备丙氨酸或使用丙氨酸生产的化合物的方法来解决，该方法具有以下步骤：（a）提供表达重组丙氨酸脱氢酶的细胞或其变体，（b）培养 在有氧条件下在无水氮源存在下在水相中，和（c）使细胞与有机相接触，所述细胞是原核或低等真核细胞。

公开(公告)号：US10450590B2

公开(公告)日：2019-10-22

申请号：US14763378

申请日：2014-01-10

申请人： Thomas Haas ,  Philip Engel ,  Jan Christoph Pfeffer ,  Oliver Thum ,  Christian Gehring

发明人： Thomas Haas ,  Philip Engel ,  Jan Christoph Pfeffer ,  Oliver Thum ,  Christian Gehring

IPC分类号： C12P7/18 ,  C07C67/08 ,  C07C29/136 ,  C12P7/62

摘要： The invention relates to a process for preparing an α,ω-alkanediol comprising the steps of a) reacting an alkanoic acid with an alkanol to give an ester, b) oxidizing at least one terminal carbon atom of the ester by contacting with a whole-cell catalyst, which expresses an alkane hydroxylase, in aqueous solution and in the presence of molecular oxygen, to give an oxidized ester, c) hydrogenating the oxidized ester to form the alkanediol and alkanol, and d) removing the alkanol by distillation, forming a reaction mixture depleted with respect to the alkanol, and recycling the alkanol in step b).

公开(公告)号：US20160244790A1

公开(公告)日：2016-08-25

申请号：US15073105

申请日：2016-03-17

申请人： Thomas HAAS ,  Jan Christoph Pfeffer ,  Kurt Faber ,  Michael Fuchs

发明人： Thomas HAAS ,  Jan Christoph Pfeffer ,  Kurt Faber ,  Michael Fuchs

IPC分类号： C12P13/00 ,  C12P17/04 ,  C12P7/22 ,  C12P17/12

CPC分类号： C12P13/001 ,  C12P7/22 ,  C12P17/04 ,  C12P17/12

摘要： A method comprising the steps (a) contacting a hydrocarbon comprising a hydroxyl group with a biological agent having oxygen-dependent and cofactor-dependent carbohydrate oxidase activity in the presence of oxygen and carbohydrate oxidase cofactor, and (b) contacting the hydrocarbon produced in step a) with a biological agent having transaminase activity and a biological agent having cofactor-dependent amino acid dehydrogenase activity in the presence of amino acid dehydrogenase cofactor and the substrate amino acid of the amino acid dehydrogenase.

摘要翻译： 一种方法，包括以下步骤：（a）在氧和碳水化合物氧化酶辅因子的存在下使包含羟基的烃与具有氧依赖性和辅因子依赖性碳水化合物氧化酶活性的生物试剂接触，和（b）使步骤 a）具有转氨酶活性的生物制剂和在氨基酸脱氢酶辅因子和氨基酸脱氢酶的底物氨基酸存在下具有辅因子依赖性氨基酸脱氢酶活性的生物试剂。

公开(公告)号：US20150111253A1

公开(公告)日：2015-04-23

申请号：US14384301

申请日：2013-03-12

申请人： Steffen Schaffer ,  Michaela Hauberg ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Harald Haeger

发明人： Steffen Schaffer ,  Michaela Hauberg ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Harald Haeger

IPC分类号： C12P7/64 ,  C07K14/21

CPC分类号： C12P7/6436 ,  C07K14/21 ,  C07K14/79 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/0077 ,  C12N9/0095 ,  C12N9/1096 ,  C12P7/42 ,  C12P7/62 ,  C12P7/6409 ,  C12P13/005 ,  C12Y114/14001

摘要： The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.

摘要翻译： 本发明涉及一种氧化式（I）的H3C-（CH2）n-COOR的脂肪酸或其酯的方法，其中R选自H，甲基，乙基，丙基和丁基，其中 n为0至30，优选6至24，包括通过在分子氧和NAD存在下使脂肪酸或其酯与CYP153家族的细胞色素P450单加氧酶接触来氧化脂肪酸或其酯的步骤 P）H和表达CYP153家族的重组细胞色素P450单加氧酶，重组醇脱氢酶，重组转氨酶以及任选的一种或多于一种重组酶的全细胞催化剂，其包含丙氨酸脱氢酶，铁氧还蛋白和铁氧还蛋白 还原酶，以及所述全细胞催化剂用于氧化脂肪酸或其酯的用途。