公开(公告)号：US20150111254A1

公开(公告)日：2015-04-23

申请号：US14390133

申请日：2013-03-25

申请人： Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Markus Pötter ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Jasmin Corthals ,  Eva-Maria Eckl ,  Silvana Roeder ,  Wolfgang Kroutil ,  Arne Skerra

发明人： Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Markus Pötter ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Jasmin Corthals ,  Eva-Maria Eckl ,  Silvana Roeder ,  Wolfgang Kroutil ,  Arne Skerra

IPC分类号： C12P13/06

CPC分类号： C12P13/06 ,  C12P13/005

摘要： The problem addressed by the invention is solved in a first aspect by a method for producing alanine or a compound produced using alanine, having the steps of (a) providing a cell which expresses a recombinant alanine dehydrogenase or a variant thereof, (b) cultivating the cell under aerobic conditions in the presence of an inorganic nitrogen source in an aqueous phase, and (c) bringing the cell into contact with an organic phase, said cell being a prokaryotic or a lower eukaryotic cell.

摘要翻译： 本发明解决的问题在第一方面通过一种制备丙氨酸或使用丙氨酸生产的化合物的方法来解决，该方法具有以下步骤：（a）提供表达重组丙氨酸脱氢酶的细胞或其变体，（b）培养 在有氧条件下在无水氮源存在下在水相中，和（c）使细胞与有机相接触，所述细胞是原核或低等真核细胞。

公开(公告)号：US09580732B2

公开(公告)日：2017-02-28

申请号：US14233505

申请日：2012-07-17

申请人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

发明人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  Y02P20/582

摘要： A method including the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, where R1 is —OH, —SH, —NH2 or —COOR2; x is at least 3; and R2 is H, alkyl or aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, where the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme. A whole cell catalyst for carrying out the method. The use of such a whole cell catalyst for oxidizing a primary alcohol.

摘要翻译： 一种方法，包括步骤a）提供式HO-（CH 2）x -R 1的伯醇，其中R 1是-OH，-SH，-NH 2或-COOR 2; x至少为3; 并且R2是H，烷基或芳基，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化伯醇，和c）使步骤a）的氧化产物与转氨酶接触，其中NAD ）+ - 醇脱氢酶和/或转氨酶是重组或分离的酶。 用于实施该方法的全细胞催化剂。 使用这种全细胞催化剂来氧化伯醇。

公开(公告)号：US20140242646A1

公开(公告)日：2014-08-28

申请号：US14233505

申请日：2012-07-17

申请人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

发明人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  Y02P20/582

摘要： The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, wherein R1 is selected from the group consisting of —OH, —SH, —NH2 and —COOR2, x is at least 3 and R2 is selected from the group consisting of H, alkyl and aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a primary alcohol.

摘要翻译： 本发明涉及包括以下步骤的方法：a）提供式HO-（CH 2）x -R 1的伯醇，其中R 1选自-OH，-SH，-NH 2和-COOR 2，x 至少3，R2选自H，烷基和芳基，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化伯醇，和c）使步骤a的氧化产物 ）与转氨酶，其中NAD（P）+ - 醇脱氢酶和/或转氨酶是重组或分离的酶，用于实施该方法的全细胞催化剂，以及使用这种全细胞催化剂进行氧化 一个小酒精。

公开(公告)号：US20150111253A1

公开(公告)日：2015-04-23

申请号：US14384301

申请日：2013-03-12

申请人： Steffen Schaffer ,  Michaela Hauberg ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Harald Haeger

发明人： Steffen Schaffer ,  Michaela Hauberg ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Harald Haeger

IPC分类号： C12P7/64 ,  C07K14/21

CPC分类号： C12P7/6436 ,  C07K14/21 ,  C07K14/79 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/0077 ,  C12N9/0095 ,  C12N9/1096 ,  C12P7/42 ,  C12P7/62 ,  C12P7/6409 ,  C12P13/005 ,  C12Y114/14001

摘要： The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.

摘要翻译： 本发明涉及一种氧化式（I）的H3C-（CH2）n-COOR的脂肪酸或其酯的方法，其中R选自H，甲基，乙基，丙基和丁基，其中 n为0至30，优选6至24，包括通过在分子氧和NAD存在下使脂肪酸或其酯与CYP153家族的细胞色素P450单加氧酶接触来氧化脂肪酸或其酯的步骤 P）H和表达CYP153家族的重组细胞色素P450单加氧酶，重组醇脱氢酶，重组转氨酶以及任选的一种或多于一种重组酶的全细胞催化剂，其包含丙氨酸脱氢酶，铁氧还蛋白和铁氧还蛋白 还原酶，以及所述全细胞催化剂用于氧化脂肪酸或其酯的用途。

公开(公告)号：US09611489B2

公开(公告)日：2017-04-04

申请号：US14384301

申请日：2013-03-12

申请人： Steffen Schaffer ,  Michaela Hauberg ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Harald Haeger

发明人： Steffen Schaffer ,  Michaela Hauberg ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Jan Christoph Pfeffer ,  Thomas Haas ,  Harald Haeger

IPC分类号： C12P7/64 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12P7/42 ,  C12P7/62 ,  C07K14/79 ,  C12N9/06 ,  C12P13/00 ,  C07K14/21

CPC分类号： C12P7/6436 ,  C07K14/21 ,  C07K14/79 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/0077 ,  C12N9/0095 ,  C12N9/1096 ,  C12P7/42 ,  C12P7/62 ,  C12P7/6409 ,  C12P13/005 ,  C12Y114/14001

摘要： The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C —(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.

公开(公告)号：US20140308717A1

公开(公告)日：2014-10-16

申请号：US14237121

申请日：2012-07-27

申请人： Thomas Haas ,  Markus Poetter ,  Jan Christoph Pfeffer ,  Wolfgang Kroutil ,  Arne Skerra ,  Alexandra Lerchner ,  Katharina Christin Tauber ,  Johann H. Sattler ,  Steffen Schaffer

发明人： Thomas Haas ,  Markus Poetter ,  Jan Christoph Pfeffer ,  Wolfgang Kroutil ,  Arne Skerra ,  Alexandra Lerchner ,  Katharina Christin Tauber ,  Johann H. Sattler ,  Steffen Schaffer

IPC分类号： C12P13/00 ,  C12P17/04 ,  C12N15/63

CPC分类号： C12P13/001 ,  C12N9/0006 ,  C12N9/1096 ,  C12N15/63 ,  C12P13/005 ,  C12P13/04 ,  C12P17/04 ,  C12Y101/01001 ,  C12Y206/01 ,  C12Y206/01018

摘要： The present invention relates to a method comprising the steps a) providing a secondary alcohol, b) oxidizing the secondary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-dependent alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a secondary alcohol.

摘要翻译： 本发明涉及包括以下步骤的方法：a）提供仲醇，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化仲醇，和c）将步骤a）的氧化产物与 转氨酶，其中NAD（P）+依赖性醇脱氢酶和/或转氨酶是重组或分离的酶，用于实施该方法的全细胞催化剂，以及使用这种全细胞催化剂来氧化二级 醇。

公开(公告)号：US09249435B2

公开(公告)日：2016-02-02

申请号：US13721481

申请日：2012-12-20

申请人： Jasmin Gielen ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

发明人： Jasmin Gielen ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

IPC分类号： C12P13/04 ,  C12P7/64 ,  C12N9/02

CPC分类号： C12P7/6436 ,  C12N9/001 ,  C12P13/04 ,  C12Y103/99003

摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

摘要翻译： 提供了一种从水性培养基中改善疏水性有机溶液分离的方法。 该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。 与野生型活性细胞相比，代谢活性细胞的活性降低，活性降低至少是催化脂肪酸一次反应的一种酶。 本发明进一步提供了一种代谢活性细胞，其与野生型相比具有降低的活性，其酶催化脂肪酸的一种反应的反应，包括选自FadA，FadB，FadD的酶 ，FadL和FadE及其变体。

公开(公告)号：US20140256904A1

公开(公告)日：2014-09-11

申请号：US14238591

申请日：2012-08-15

申请人： Steffen Schaffer ,  Nicole Decker ,  Jasmin Gielen ,  Harald Haeger ,  Thomas Haas ,  Markus Poetter ,  Hans-Georg Hennemann ,  Mirja Wessel ,  Michael Volland

发明人： Steffen Schaffer ,  Nicole Decker ,  Jasmin Gielen ,  Harald Haeger ,  Thomas Haas ,  Markus Poetter ,  Hans-Georg Hennemann ,  Mirja Wessel ,  Michael Volland

IPC分类号： C12P13/00 ,  C12P13/02 ,  C08G69/08 ,  C12P7/64

CPC分类号： C12P13/02 ,  C08G69/08 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0014 ,  C12N9/0077 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/40 ,  C12P7/42 ,  C12P7/6409 ,  C12P7/6436 ,  C12P13/005 ,  Y02P20/52

摘要： The subject of the invention is a biotechnological process for the production of ω-functionalized carboxylic acids and of ω-functionalized carboxylate esters from simple carbon sources.

摘要翻译： 本发明的主题是用于从简单碳源生产ω官能化羧酸和ω-官能化羧酸酯的生物技术方法。

公开(公告)号：US09719117B2

公开(公告)日：2017-08-01

申请号：US14132473

申请日：2013-12-18

申请人： Steffen Schaffer ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

发明人： Steffen Schaffer ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

IPC分类号： C12P13/00 ,  C12N9/12 ,  C12N9/02 ,  C12N9/06 ,  C12N9/10

CPC分类号： C12P13/001 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/0069 ,  C12N9/1096 ,  C12N9/1288 ,  C12P13/005 ,  Y02P20/582

摘要： The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

公开(公告)号：US20130164797A1

公开(公告)日：2013-06-27

申请号：US13721481

申请日：2012-12-20

申请人： Jasmin GIELEN ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

发明人： Jasmin GIELEN ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

IPC分类号： C12P7/64

CPC分类号： C12P7/6436 ,  C12N9/001 ,  C12P13/04 ,  C12Y103/99003

摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

摘要翻译： 提供了一种从水性培养基中改进疏水性有机溶液分离的方法。 该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。 代谢活性细胞的活性降低与野生型活性细胞相比，活性降低至少是一种催化脂肪酸β-氧化反应的一种酶。 本发明还提供了一种代谢活性细胞，其与野生型相比具有降低的活性，所述活性细胞能催化脂肪酸的β-氧化反应之一，包括选自FadA，FadB，FadD， FadL和FadE及其变体。