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    ENZYMATIC AMINATION
    1.
    发明申请
    ENZYMATIC AMINATION 审中-公开
    酶联系

    公开(公告)号：US20160244790A1

    公开(公告)日：2016-08-25

    申请号：US15073105

    申请日：2016-03-17

    申请人： Thomas HAAS ,  Jan Christoph Pfeffer ,  Kurt Faber ,  Michael Fuchs

    发明人： Thomas HAAS ,  Jan Christoph Pfeffer ,  Kurt Faber ,  Michael Fuchs

    IPC分类号： C12P13/00 ,  C12P17/04 ,  C12P7/22 ,  C12P17/12

    CPC分类号： C12P13/001 ,  C12P7/22 ,  C12P17/04 ,  C12P17/12

    摘要： A method comprising the steps (a) contacting a hydrocarbon comprising a hydroxyl group with a biological agent having oxygen-dependent and cofactor-dependent carbohydrate oxidase activity in the presence of oxygen and carbohydrate oxidase cofactor, and (b) contacting the hydrocarbon produced in step a) with a biological agent having transaminase activity and a biological agent having cofactor-dependent amino acid dehydrogenase activity in the presence of amino acid dehydrogenase cofactor and the substrate amino acid of the amino acid dehydrogenase.

    摘要翻译： 一种方法，包括以下步骤：（a）在氧和碳水化合物氧化酶辅因子的存在下使包含羟基的烃与具有氧依赖性和辅因子依赖性碳水化合物氧化酶活性的生物试剂接触，和（b）使步骤 a）具有转氨酶活性的生物制剂和在氨基酸脱氢酶辅因子和氨基酸脱氢酶的底物氨基酸存在下具有辅因子依赖性氨基酸脱氢酶活性的生物试剂。

    ENZYMATIC AMINATION
    2.
    发明申请
    ENZYMATIC AMINATION 审中-公开
    酶联系

    公开(公告)号：US20140120587A1

    公开(公告)日：2014-05-01

    申请号：US14126607

    申请日：2012-01-10

    申请人： Thomas Haas ,  Jan Christoph Pfeffer ,  Kurt Faber ,  Michael Fuchs

    发明人： Thomas Haas ,  Jan Christoph Pfeffer ,  Kurt Faber ,  Michael Fuchs

    IPC分类号： C12P17/12 ,  C12P13/00 ,  C12P17/04

    CPC分类号： C12P13/001 ,  C12P7/22 ,  C12P17/04 ,  C12P17/12

    摘要： A method comprising the steps (a) contacting a hydrocarbon comprising a hydroxyl group with a biological agent having oxygen-dependent and cofactor-dependent carbohydrate oxidase activity in the presence of oxygen and carbohydrate oxidase cofactor, and (b) contacting the hydrocarbon produced in step a) with a biological agent having transaminase activity and a biological agent having cofactor-dependent amino acid dehydrogenase activity in the presence of amino acid dehydrogenase cofactor and the substrate amino acid of the amino acid dehydrogenase.

    摘要翻译： 一种方法，包括以下步骤：（a）在氧和碳水化合物氧化酶辅因子的存在下使包含羟基的烃与具有氧依赖性和辅因子依赖性碳水化合物氧化酶活性的生物试剂接触，和（b）使步骤 a）具有转氨酶活性的生物制剂和在氨基酸脱氢酶辅因子和氨基酸脱氢酶的底物氨基酸存在下具有辅因子依赖性氨基酸脱氢酶活性的生物试剂。

    CONTINUOUSLY OPERABLE METHOD FOR PRODUCING CARBONYL COMPOUNDS BY MEANS OF A CATALYST CONTAINING A NITROXYL RADICAL
    5.
    发明申请
    CONTINUOUSLY OPERABLE METHOD FOR PRODUCING CARBONYL COMPOUNDS BY MEANS OF A CATALYST CONTAINING A NITROXYL RADICAL 有权
    通过含有硝基吡咯的催化剂生产碳化合物的连续可操作方法

    公开(公告)号：US20140039223A1

    公开(公告)日：2014-02-06

    申请号：US14110450

    申请日：2011-12-06

    申请人： Florian Klasovsky ,  Thomas Haas ,  Thomas Tacke ,  Jan Christoph Pfeffer ,  Michael Rimbach ,  Michael Volland ,  Michiel Janssen ,  Roger Sheldon ,  Juergen Haberland

    发明人： Florian Klasovsky ,  Thomas Haas ,  Thomas Tacke ,  Jan Christoph Pfeffer ,  Michael Rimbach ,  Michael Volland ,  Michiel Janssen ,  Roger Sheldon ,  Juergen Haberland

    IPC分类号： C07C45/59

    CPC分类号： C07C45/59 ,  C07D493/04

    摘要： The invention relates to a method for the oxidation of a primary or secondary alcohol, preferably to form an aldehyde or ketone, comprising the following steps: a) providing a catalyst composition comprising at least one compound containing a nitroxyl radical, at least one NO source, at least one carbon or mineral acid or an anhydride of a carbon or mineral acid; b) producing a reaction mixture by adding at least one primary or secondary alcohol and a gas comprising oxygen and optionally one or more than one solvent to the catalyst composition from step a) or step e); c) incubating the reaction mixture from step b) at a temperature of between 0 and 100° C. or at the boiling point of the solvent; d) simultaneously with or subsequent to step c): crystallizing the reaction product; and e) recovering the catalyst composition by removing the crystallized reaction product from the reaction mixture obtained in step d).

    摘要翻译： 本发明涉及氧化伯醇或仲醇，优选形成醛或酮的方法，包括以下步骤：a）提供包含至少一种含有硝酰基的化合物的催化剂组合物，至少一种NO源 ，至少一种碳或无机酸或碳或无机酸的酸酐; b）通过从步骤a）或步骤e）向催化剂组合物中加入至少一种伯醇或仲醇和包含氧气和任选的一种或多种溶剂的气体产生反应混合物; c）在0-100℃的温度或溶剂的沸点下温育来自步骤b）的反应混合物; d）与步骤c）同时或之后步骤：使反应产物结晶; 和e）通过从步骤d）中获得的反应混合物中除去结晶的反应产物来回收催化剂组合物。

    BIOTECHNOLOGICAL PREPARATION OF 3-HYDROXYISOBUTYRIC ACID
    6.
    发明申请
    BIOTECHNOLOGICAL PREPARATION OF 3-HYDROXYISOBUTYRIC ACID 审中-公开
    3-羟基丁酸的生物技术制备

    公开(公告)号：US20150218600A1

    公开(公告)日：2015-08-06

    申请号：US14363178

    申请日：2012-11-14

    申请人： Thomas Haas ,  Steffen Schaffer ,  Markus Poetter ,  Mirja Wessel ,  Jan Christoph Pfeffer ,  Christian Gehring ,  Nicole Kirchner ,  Eva Maria Wittmann

    发明人： Thomas Haas ,  Steffen Schaffer ,  Markus Poetter ,  Mirja Wessel ,  Jan Christoph Pfeffer ,  Christian Gehring ,  Nicole Kirchner ,  Eva Maria Wittmann

    IPC分类号： C12P7/42 ,  C12N9/88 ,  C12N9/12 ,  C12N9/02 ,  C12N9/00 ,  C12N9/10

    CPC分类号： C12P7/42 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/1217 ,  C12N9/13 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12Y103/99002 ,  C12Y207/02007 ,  C12Y402/01017 ,  C12Y602/01002

    摘要： The invention relates to a method comprising the steps a) providing isobutyric acid, b) bringing isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A synthetase/ligase and/or isobutyrate-coenzyme A transferase, c) bringing the product from step a) into contact with isobutyryl-coenzyme A dehydrogenase, d) bringing the product from step b) into contact with methacrylyl-coenzyme A hydratase, and e) hydrolyzing the product from step d) to form 3-hydroxyisobutyric acid, where at least one of the enzymes is used in the form of a cell which, compared to its wildtype, comprises a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, a cell which has at least one enzyme from the group comprising isobutyryl-coenzyme A synthetase/ligase, isobutyrate-coenzyme A transferase, isobutyrate kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A dehydrogenase, methacrylyl-coenzyme A hydratase and 3-hydroxyisobutyryl-coenzyme A hydrolase and, compared to its wildtype, a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, wherein the cell preferably has, in addition, a monooxygenase, more preferably a monooxygenase of the alkBGT type or a variant thereof and the use of such a cell for preparing 3-hydroxyisobutyric acid.

    摘要翻译： 本发明涉及包括以下步骤的方法：a）提供异丁酸，b）使异丁酸与异丁酸酯激酶和磷酸转移异丁酰基酶和/或异丁酰辅酶A合成酶/连接酶和/或异丁酸酯辅酶A转移酶c的组合接触 ）使来自步骤a）的产物与异丁酰辅酶A脱氢酶接触，d）将来自步骤b）的产物与甲基丙烯酰辅酶A水合酶接触，和e）水解步骤d）中的产物以形成3-羟基异丁酸 酸，其中至少一种酶以细胞形式使用，与其野生型相比，其包含3-羟基异丁酸脱氢酶或其变体的降低的活性，具有至少一种酶的细胞 异丁酰辅酶A合成酶/连接酶，异丁酸酯辅酶A转移酶，异丁酸酯激酶，磷酸转移异丁酰基酶，异丁酰辅酶A脱氢酶，甲基丙烯酰辅酶A水合酶和 3-羟基异丁酰辅酶A水解酶，与其野生型相比，3-羟基异丁酸脱氢酶或其变体的活性降低，其中细胞优选还具有单加氧酶，更优选alkBGT型的单加氧酶或 其变体以及这种细胞用于制备3-羟基异丁酸的用途。