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公开(公告)号:US20070119711A1
公开(公告)日:2007-05-31
申请号:US11698363
申请日:2007-01-25
申请人: Walter Ausserer , Luc Bousse , Robert Dubrow , Steven Sundberg , Andrea Chow , Benjiamin Wang
发明人: Walter Ausserer , Luc Bousse , Robert Dubrow , Steven Sundberg , Andrea Chow , Benjiamin Wang
CPC分类号: C07K1/26 , G01N27/447
摘要: Methods and systems for use in separating sample materials into different fractions employing pressure-based fluid flow for simultaneous loading of a sample and a reagent into a sample loading channel of a microfluidic device. The sample is loaded from an external source through an attached external sampling capillary. The reagent, which may be a molecular weight standard, a diluent, a detergent, or a labeling reagent, is loaded from a reservoir integral to the microfluidic device via a reagent introduction channel within the device. The sample and reagent form a mixture in the sample loading channel. A portion of the mixture is electrokinetically injected from the sample loading channel, via an injection channel, into a separation channel, where it is separated electrophoretically.
摘要翻译: 用于将样品材料分离成不同级分的方法和系统,其使用基于压力的流体流将样品和试剂同时装载到微流体装置的样品加载通道中。 样品通过附带的外部取样毛细管从外部来源加载。 可以是分子量标准的试剂,稀释剂,洗涤剂或标记试剂,通过装置内的试剂引入通道从集成在微流体装置中的储存器装载。 样品和试剂在样品加载通道中形成混合物。 将混合物的一部分从样品加载通道经由注射通道电动注射到分离通道中,其中电泳分离。
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公开(公告)号:US07169277B2
公开(公告)日:2007-01-30
申请号:US09919505
申请日:2001-07-31
申请人: Walter Ausserer , Luc J. Bousse , Robert S. Dubrow , Steven A. Sundberg , Andrea W. Chow , Benjamin N. Wang
发明人: Walter Ausserer , Luc J. Bousse , Robert S. Dubrow , Steven A. Sundberg , Andrea W. Chow , Benjamin N. Wang
IPC分类号: G01N27/26
CPC分类号: G01N27/44791 , G01N27/44743
摘要: Devices, systems and methods for use in separating sample materials into different fractions that employ bulk fluid flow for loading of samples followed by electrophoretic separation of the sample material. Devices employ configurations that optionally allow bulk sample loading with some or no displacement of a separation matrix within a separation conduit. Methods of using these devices, and systems that incorporate these devices are also envisioned.
摘要翻译: 用于将样品材料分离成不同级分的装置,系统和方法,其使用大量流体流来装载样品,然后电泳分离样品材料。 设备采用可选地允许在分离管道内部分或不排出分离基质的大量样品加载的构造。 也可以设想使用这些装置的方法以及包含这些装置的系统。
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