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    Microchip large-volume PCR with integrated real-time CE detection
    1.
    发明授权
    Microchip large-volume PCR with integrated real-time CE detection 有权
    Microchip大容量PCR，集成实时CE检测

    公开(公告)号：US08394324B2

    公开(公告)日：2013-03-12

    申请号：US12664018

    申请日：2008-06-11

    申请人： Luc Bousse ,  Jian-ping Zhang

    发明人： Luc Bousse ,  Jian-ping Zhang

    IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  C12Q1/68 ,  C12P19/34 ,  C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  C12M1/34

    CPC分类号： G01N27/44791 ,  B01L3/502746 ,  B01L3/502753 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2400/0421 ,  G01N27/44721

    摘要： A microfluidic device is provided with appropriate integrated structures to conduct large volume PCR and end-point or real-time capillary electrophoresis detection. The microfluidic device includes a substrate having an amplification chamber of a volume of nucleic acid, wells disposed on the substrate, flow channels connecting the wells and the chamber in the substrate to allow for solution flow through the chamber, and one or more separation channels provided in the substrate and connected to the chamber for separating and detecting a fraction of the amplified nucleic acid. The chamber, the flow channels, and the one or more separation channels are configured such that the hydrodynamic flow resistance of the chambers and the flow channels combined is at least 10Λ3 times smaller than the hydrodynamic flow resistance in the one or more separation channels. The microfluidic device can achieve a very high sensitivity in detection while being highly cost effective.

    摘要翻译： 微流体装置具有适当的整合结构，可进行大容量PCR和终点或实时毛细管电泳检测。 微流体装置包括具有一定体积的核酸的扩增室的衬底，设置在衬底上的阱，连通阱的流动通道和衬底中的室，以允许溶液流过腔室，以及提供一个或多个分离通道 在底物中并连接到室以分离和检测一部分扩增的核酸。 腔室，流动通道和一个或多个分离通道被构造成使得腔室和流动通道组合的流体动力学流动阻力比一个或多个分离通道中的流体动力流动阻力小至少10 ^ 3倍。 微流体装置在检测中可以获得非常高的灵敏度，同时具有高成本效益。

    ELECTROSPRAY APPARATUS WITH AN INTEGRATED ELECTRODE
    2.
    发明申请
    ELECTROSPRAY APPARATUS WITH AN INTEGRATED ELECTRODE 审中-公开
    具有集成电极的电绝缘装置

    公开(公告)号：US20080235948A1

    公开(公告)日：2008-10-02

    申请号：US12056109

    申请日：2008-03-26

    申请人： Luc Bousse ,  Mingqi Zhao

    发明人： Luc Bousse ,  Mingqi Zhao

    IPC分类号： B23P17/00

    CPC分类号： H01J49/167 ,  B05B5/025 ,  B05B5/0533 ,  H01J49/0018 ,  Y10T29/494

    摘要： The invention provides related apparatus and methods of making an integrated electrospray tip by depositing ionic and/or electronic conductor materials onto a planar substrate. The invention also features methods of forming an electrospray apparatus comprising coupling a first planar substrate to the surface of a second planar substrate, wherein a surface on at least one of the substrates includes one or more microfluidic channels and/or reservoirs which are at least partially or totally enclosed therebetween. The conductive regions of the apparatus do not intersect the microfluidic channels within other portions of the apparatus provided preferably. The invention further provides related apparatus and methods for manufacturing and using microfluidic devices with integrated electrodes for electrospray ionization. The electrospray apparatus in some embodiments may include an electronic conductor electrode or an ionic conductor electrode formed from a microfluidic channel containing a conductive material selected from a variety of solutions and gels.

    摘要翻译： 本发明提供了通过将离子和/或电子导体材料沉积到平面衬底上来制造集成电喷雾尖端的相关装置和方法。 本发明还具有形成电喷射装置的方法，其包括将第一平面基底耦合到第二平面基底的表面，其中至少一个基底上的表面包括一个或多个微流体通道和/或至少部分地 或完全封闭在其间。 设备的导电区域优选地不与装置的其它部分内的微流体通道相交。 本发明还提供了用于制造和使用具有用于电喷雾电离的集成电极的微流体装置的相关装置和方法。 在一些实施例中，电喷雾装置可以包括由包含选自多种溶液和凝胶的导电材料的微流体通道形成的电子导体电极或离子导体电极。

    Electrospray apparatus with an integrated electrode
    4.
    发明申请
    Electrospray apparatus with an integrated electrode 审中-公开
    具有集成电极的电喷设备

    公开(公告)号：US20060060769A1

    公开(公告)日：2006-03-23

    申请号：US11031963

    申请日：2005-01-06

    申请人： Luc Bousse ,  Mingqi Zhao

    发明人： Luc Bousse ,  Mingqi Zhao

    IPC分类号： B01D59/44

    CPC分类号： H01J49/167 ,  B05B5/025 ,  B05B5/0533 ,  H01J49/0018 ,  Y10T29/494

    摘要： The invention provides related apparatus and methods of making an integrated electrospray tip by depositing ionic and/or electronic conductor materials onto a planar substrate. The invention also features methods of forming an electrospray apparatus comprising coupling a first planar substrate to the surface of a second planar substrate, wherein a surface on at least one of the substrates includes one or more microfluidic channels and/or reservoirs which are at least partially or totally enclosed therebetween. The conductive regions of the apparatus do not intersect the microfluidic channels within other portions of the apparatus provided preferably. The invention further provides related apparatus and methods for manufacturing and using microfluidic devices with integrated electrodes for electrospray ionization. The electrospray apparatus in some embodiments may include an electronic conductor electrode or an ionic conductor electrode formed from a microfluidic channel containing a conductive material selected from a variety of solutions and gels.

    摘要翻译： 本发明提供了通过将离子和/或电子导体材料沉积到平面衬底上来制造集成电喷雾尖端的相关装置和方法。 本发明还具有形成电喷射装置的方法，其包括将第一平面基底耦合到第二平面基底的表面，其中至少一个基底上的表面包括一个或多个微流体通道和/或至少部分地 或完全封闭在其间。 设备的导电区域优选地不与装置的其它部分内的微流体通道相交。 本发明还提供了用于制造和使用具有用于电喷雾电离的集成电极的微流体装置的相关装置和方法。 在一些实施例中，电喷雾装置可以包括由包含选自多种溶液和凝胶的导电材料的微流体通道形成的电子导体电极或离子导体电极。

    Microfluidic chip with enhanced tip for stable electrospray ionization
    6.
    发明授权
    Microfluidic chip with enhanced tip for stable electrospray ionization 失效
    具有增强尖端的微流控芯片，用于稳定的电喷雾电离

    公开(公告)号：US07105812B2

    公开(公告)日：2006-09-12

    申请号：US10871498

    申请日：2004-06-18

    申请人： Mingqi Zhao ,  Iuliu Blaga ,  Luc Bousse ,  John Stults ,  Jing Ni

    发明人： Mingqi Zhao ,  Iuliu Blaga ,  Luc Bousse ,  John Stults ,  Jing Ni

    IPC分类号： H01J49/04 ,  B01D59/44

    CPC分类号： H01J49/167 ,  B01L3/0268 ,  B01L3/502715 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/027 ,  B81B1/008 ,  B81B2201/058 ,  G01N27/44717 ,  H01J49/0018

    摘要： A microfluidic chip formed with multiple fluid channels terminating at a tapered electrospray ionization tip for mass spectrometric analysis. The fluid channels may be formed onto a channel plate that are in fluid communication with corresponding reservoirs. The electrospray tip can be formed along a defined distal portion of the channel plate that can include a single or multiple tapered surfaces. The fluid channels may terminate at an open-tip region of the electrospray tip. A covering plate may substantially enclose most portions of the fluid channels formed on the channel plate except for the open-tip region. Another aspect of the invention provides methods for conducting mass spectrometric analysis of multiple samples flowing through individual fluid channels in a single microfluidic chip that is formed with a tapered electrospray tip having an open-tip region.

    摘要翻译： 微流体芯片形成有多个流体通道，终止于锥形电喷雾离子化尖端，用于质谱分析。 流体通道可以形成在与相应的储存器流体连通的通道板上。 电喷雾尖端可以沿着通道板的限定的远侧部分形成，其可以包括单个或多个锥形表面。 流体通道可以终止于电喷雾尖端的开口尖端区域。 覆盖板可以基本上封闭形成在通道板上的除了开口尖端区域之外的流体通道的大部分。 本发明的另一方面提供了在单个微流体芯片中流过单个流体通道的多个样品进行质谱分析的方法，所述单个微流体芯片由具有开口尖端区域的锥形电喷雾尖端形成。

    Microfluidic devices and methods with integrated electrical contact
    7.
    发明申请
    Microfluidic devices and methods with integrated electrical contact 审中-公开
    微流控器件和集成电接触方法

    公开(公告)号：US20060022130A1

    公开(公告)日：2006-02-02

    申请号：US10903248

    申请日：2004-07-29

    申请人： Luc Bousse ,  Mingqi Zhao ,  Jing Ni

    发明人： Luc Bousse ,  Mingqi Zhao ,  Jing Ni

    IPC分类号： H01J49/00

    CPC分类号： H01J49/165 ,  B01L3/0268 ,  B01L3/5027 ,  H01J49/0018

    摘要： Microfluidic devices provide substances to a mass spectrometer. The microfluidic devices include a substrate having at least one microchannel, a cover arranged on a surface of the microchannel, and at least one electrical potential source. Some embodiments include a microchannel widened at an outlet. Other embodiments position the electrical potential source along a surface of the cover. Still other embodiments include a well in which an electrode and a membrane are disposed. The various embodiments provide stable electrospray ionization of substances from a microfluidic device to a mass spectrometer.

    摘要翻译： 微流体装置向质谱仪提供物质。 微流体装置包括具有至少一个微通道的衬底，布置在微通道表面上的盖和至少一个电势源。 一些实施例包括在出口处加宽的微通道。 其他实施例将电势源沿着盖的表面定位。 其他实施例包括其中设置电极和膜的阱。 各种实施方案提供物质从微流体装置到质谱仪的稳定的电喷雾电离。

    MICROCHIP LARGE-VOLUME PCR WITH INTEGRATED REAL-TIME CE DETECTION
    9.
    发明申请
    MICROCHIP LARGE-VOLUME PCR WITH INTEGRATED REAL-TIME CE DETECTION 有权
    MICROCHIP大容量PCR与一体化实时检测

    公开(公告)号：US20100173310A1

    公开(公告)日：2010-07-08

    申请号：US12664018

    申请日：2008-06-11

    申请人： Luc Bousse ,  Jian-ping Zhang

    发明人： Luc Bousse ,  Jian-ping Zhang

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12P19/34

    CPC分类号： G01N27/44791 ,  B01L3/502746 ,  B01L3/502753 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2400/0421 ,  G01N27/44721

    摘要： A microfluidic device is provided with appropriate integrated structures to conduct large volume PCR and end-point or real-time capillary electrophoresis detection The microfluidic device includes a substrate having an amplification chamber of a volume of nucleic acid, wells disposed on the substrate, flow channels connecting the wells and the chamber in the substrate to allow for solution flow through the chamber, and one or more separation channels provided in the substrate and connected to the chamber for separating and detecting a fraction of the amplified nucleic acid The chamber, the flow channels, and the one or more separation channels are configured such that the hydrodynamic flow resistance of the chambers and the flow channels combined is at least 10Λ3 times smaller than the hydrodynamic flow resistance in the one or more separation channels The microfluidic device can achieve a very high sensitivity in detection while being highly cost effective

    摘要翻译： 微流体装置具有适当的集成结构以进行大容量PCR和终点或实时毛细管电泳检测微流体装置包括具有一定体积核酸的扩增室的底物，设置在基底上的阱，流路 将所述孔和所述腔室连接在所述基底中以允许溶液流过所述腔室，以及设置在所述基底中并连接到所述腔室的一个或多个分离通道，用于分离和检测所述扩增的核酸的一部分所述腔室，所述流动通道 ，并且所述一个或多个分离通道被配置为使得组合的腔室和流动通道的流体动力学流动阻力比一个或多个分离通道中的流体动力流动阻力小至少10 ^ 3倍。微流体装置可以实现非常高的 检测灵敏度高，成本效益高

    Optimized sample injection structures in microfluidic separations
    10.
    发明授权
    Optimized sample injection structures in microfluidic separations 有权
    微流体分离中优化的样品注射结构

    公开(公告)号：US07749365B2

    公开(公告)日：2010-07-06

    申请号：US11460236

    申请日：2006-07-26

    申请人： Michael Nguyen ,  Luc Bousse

    发明人： Michael Nguyen ,  Luc Bousse

    IPC分类号： G01N27/26 ,  B81B7/00

    CPC分类号： C07K1/26 ,  G01N27/44791 ,  Y10T436/2575

    摘要： The invention herein provides improved sample injection systems and related methods to create microfluidic devices with symmetrical channel configurations that can produce relatively large sample volumes. An embodiment of the invention provides microfluidic structures with different geometries that are symmetrical from the perspective of a sample load channel and a sample waste channel, which essentially eliminates issues of time offset and other problems commonly associated with twin-T sample formation techniques. A split-injection approach and related methods of sample plug formation are therefore provided.

    摘要翻译： 本文的发明提供了改进的样品注射系统和相关方法来产生具有对称通道构型的微流体装置，其可以产生相对较大的样品体积。 本发明的一个实施方案提供了具有不同几何形状的微流体结构，其从样品负载通道和样品废物通道的角度对称，其基本上消除了时间偏移和通常与双T样品形成技术相关的其它问题的问题。 因此提供了分离注射方法和样品塞形成的相关方法。