公开(公告)号：US20120288865A1

公开(公告)日：2012-11-15

申请号：US13444502

申请日：2012-04-11

Applicant: Steven A. Sundberg ,  Michael R. Knapp ,  Ivor T. Knight ,  Deborah J. Boles ,  Aaron J. Rulison ,  Wesley B. Dong ,  Edward P. Donlon ,  Robert J. Moti ,  Andrew G. Fabans ,  Allen R. Boronkay ,  Michael Slater

Inventor： Steven A. Sundberg ,  Michael R. Knapp ,  Ivor T. Knight ,  Deborah J. Boles ,  Aaron J. Rulison ,  Wesley B. Dong ,  Edward P. Donlon ,  Robert J. Moti ,  Andrew G. Fabans ,  Allen R. Boronkay ,  Michael Slater

IPC: G01N25/04

CPC classification number: G01N25/04 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1833 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6862 ,  G01N21/33 ,  G01N21/6428 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q2527/101

Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

Abstract translation: 本发明提供了采用微流体技术产生分子熔融曲线的新颖方法和装置。 特别地，根据本发明的装置和方法可用于提供PCR扩增产物的分析。

公开(公告)号：US07399448B2

公开(公告)日：2008-07-15

申请号：US11493998

申请日：2006-07-27

Applicant: Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

Inventor： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC: G01N1/10 ,  B01L3/02

CPC classification number: G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US07364915B2

公开(公告)日：2008-04-29

申请号：US11617804

申请日：2006-12-29

Applicant: Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

Inventor： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC: G01N1/10 ,  B01L3/02

CPC classification number: G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US06709559B2

公开(公告)日：2004-03-23

申请号：US10209493

申请日：2002-07-30

Applicant: Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

Inventor： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC: G01N2726

CPC classification number: G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

Abstract translation: 通过使用完全延伸通过微流体衬底的端口来促进流体引入。 可以使用毛细管力将流体保持在端口内，并且可以通过单个端口引入一系列样品或其它流体，通过依次将流体通过基底吹出并用替代流体代替去除的流体，或通过置换 流体部分附加流体。 在另一方面，微流体衬底具有在横截面尺寸上变化的通道，使得毛细管作用仅在通道网络的有限部分内扩散流体。 在另一方面，引入端口可以包括从端口引导到流体通道的多个非常小的通道，以便过滤除了通道和引入端口之间的连接处可能阻挡通道的颗粒或其它污染物 。

公开(公告)号：US06524790B1

公开(公告)日：2003-02-25

申请号：US09093542

申请日：1998-06-08

Applicant: Anne R. Kopf-Sill ,  Andrea W. Chow ,  Claudia B. Cohen ,  Steven A. Sundberg ,  John Wallace Parce

Inventor： Anne R. Kopf-Sill ,  Andrea W. Chow ,  Claudia B. Cohen ,  Steven A. Sundberg ,  John Wallace Parce

IPC: C12G168

CPC classification number: G01N33/557 ,  B01J19/0093 ,  B01J2219/0095 ,  B01J2219/0097 ,  B01J2219/00995 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/563 ,  B01L9/527 ,  B01L2200/14 ,  B01L2200/143 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  G01N27/447 ,  G01N27/44726 ,  G01N27/44791 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/809

Abstract: Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.

Abstract translation: 提供了具有用于校正电动效果的计算机的电动装置。 还提供了通过在电动微流体装置中确定反应物和产物在反应中的速度来校正电动效应的方法。 这些微流体装置可以具有用于移动，混合和监测精确量的分析物流体的通道，凹陷和/或孔的基底。

公开(公告)号：US06326083B1

公开(公告)日：2001-12-04

申请号：US09264519

申请日：1999-03-08

Applicant: Hua Yang ,  Steven A. Sundberg

Inventor： Hua Yang ,  Steven A. Sundberg

IPC: B32B1706

Abstract: Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.

Abstract translation: 提供了用于微流体装置的亲水性蛋白质吸附抗性涂层。 另外，提供了包括涂层的微流体装置和制造微流体装置的方法。

公开(公告)号：US06274089B1

公开(公告)日：2001-08-14

申请号：US09093489

申请日：1998-06-08

Applicant: Andrea W. Chow ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Steven A. Sundberg

Inventor： Andrea W. Chow ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Steven A. Sundberg

IPC: G01N2726

CPC classification number: B01L3/50273 ,  B01F13/0059 ,  B01L3/502753 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0421

Abstract: Microfluidic devices for performing integrated reaction and separation operations. The devices include a planar substrate having a first surface with an integrated channel network disposed therein. The reaction region in the integrated microscale channel network has a mixture of at least first and second reactants located therein, wherein the mixture interacts to produce one or more products. The reaction region is configured to maintain contact between the first and second reactants contained within it. The device also includes a separation region in the integrated channel network, where the separation region is configured to separate the first reactant from the product, when the first reactant and product are flowing through the separation region. The conductivity of a fluid in the reaction region is higher than the conductivity of a fluid in the separation region.

Abstract translation: 用于进行集成反应和分离操作的微流体装置。 这些装置包括具有设置在其中的集成通道网络的第一表面的平面基板。 集成微量通道网络中的反应区域具有位于其中的至少第一和第二反应物的混合物，其中混合物相互作用以产生一种或多种产物。 反应区域被配置为保持包含在其中的第一和第二反应物之间的接触。 该装置还包括在集成通道网络中的分离区域，其中当第一反应物和产物流过分离区域时，分离区域被配置为将第一反应物与产物分离。 反应区域中的流体的电导率高于分离区域中的流体的电导率。

公开(公告)号：US6132685A

公开(公告)日：2000-10-17

申请号：US132096

申请日：1998-08-10

Applicant: Joseph E. Kercso ,  Steven A. Sundberg ,  Jeffrey A. Wolk ,  Andrew W. Toth ,  Calvin Y. H. Chow ,  J. Wallace Parce

Inventor： Joseph E. Kercso ,  Steven A. Sundberg ,  Jeffrey A. Wolk ,  Andrew W. Toth ,  Calvin Y. H. Chow ,  J. Wallace Parce

IPC: G01N35/00 ,  G01N35/02

CPC classification number: G01N35/028 ,  G01N2035/00148 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/119163 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides improved systems, devices, and methods for analyzing a large number of sample compounds contained in standard multiwell microtiter plates or other array structures. The multiwell plates travel along a conveyor system to a test station having a microfluidic device. At the test station, each plate is removed from the conveyor and the wells of the multiwell plate are sequentially aligned with an input port of the microfluidic device. After at least a portion of each sample has been input into the microfluidic channel system, the plate is returned to the conveyor system. Pre and/or post testing stations may be disposed along the conveyor system, and the use of an X-Y-Z robotic arm and novel plate support bracket allows each of the samples in the wells to be input into the microfluidic network through a probe affixed to a microfluidic chip. A clamshell structure having a hinged lid can releasably support the chip while providing and/or accommodating the electrical, optical, structural, and other interface connections between the microfluidic device and the surrounding system.

Abstract translation: 本发明提供用于分析标准多孔微量滴定板或其它阵列结构中所含的大量样品化合物的改进的系统，装置和方法。 多孔板沿传送系统行进到具有微流体装置的测试台。 在试验台，每个板从输送机上取出，多孔板的孔依次与微流体装置的输入端对准。 在每个样品的至少一部分已经被输入到微流体通道系统中之后，板返回到输送系统。 可以沿输送机系统布置前后测试站，并且使用XYZ机器人臂和新的板支撑支架允许孔中的每个样品通过固定在微流体上的探针输入微流体网络 芯片。 具有铰接盖的蛤壳结构可以可释放地支撑芯片，同时提供和/或容纳微流体装置和周围系统之间的电，光学，结构和其它界面连接。

公开(公告)号：US6090251A

公开(公告)日：2000-07-18

申请号：US870944

申请日：1997-06-06

Applicant: Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

Inventor： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC: B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  G01N27/447 ,  G01N35/00 ,  G01N35/10 ,  G01N27/26

CPC classification number: G01N27/44791 ,  B01J19/0093 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  G01N27/44743 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L3/502723 ,  B01L3/502753 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US5624711A

公开(公告)日：1997-04-29

申请号：US431196

申请日：1995-04-27

Applicant: Steven A. Sundberg ,  David Fujimoto

Inventor： Steven A. Sundberg ,  David Fujimoto

IPC: B01J19/00 ,  B05D1/18 ,  C03C17/30 ,  C03C17/34 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/543 ,  B05D1/36

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B05D1/185 ,  B82Y40/00 ,  C03C17/30 ,  C03C17/3405 ,  C40B80/00 ,  G01N33/54353 ,  B01J2219/00364 ,  B01J2219/00378 ,  B01J2219/0043 ,  B01J2219/005 ,  B01J2219/00504 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

Abstract: Methods and derivatized supports which are useful in solid-phase synthesis of peptides, oligonucleotides or other small organic molecules as well as arrays of ligands. The methods provide means to control the functional site density on a solid support. Some of the derivatized supports are polymer-coated or glycan-coated. Other methods for regenerating the surface of a used ligand array are also provided.

Abstract translation: 可用于肽，寡核苷酸或其他小有机分子的固相合成以及配体阵列的方法和衍生的载体。 该方法提供了控制固体支持物上的功能位点密度的方法。 一些衍生的载体是聚合物涂覆的或聚糖包被的。 还提供了用于再生所用配体阵列的表面的其它方法。