公开(公告)号：US20070119711A1

公开(公告)日：2007-05-31

申请号：US11698363

申请日：2007-01-25

申请人： Walter Ausserer ,  Luc Bousse ,  Robert Dubrow ,  Steven Sundberg ,  Andrea Chow ,  Benjiamin Wang

发明人： Walter Ausserer ,  Luc Bousse ,  Robert Dubrow ,  Steven Sundberg ,  Andrea Chow ,  Benjiamin Wang

IPC分类号： C07K1/26 ,  G01N27/00

CPC分类号： C07K1/26 ,  G01N27/447

摘要： Methods and systems for use in separating sample materials into different fractions employing pressure-based fluid flow for simultaneous loading of a sample and a reagent into a sample loading channel of a microfluidic device. The sample is loaded from an external source through an attached external sampling capillary. The reagent, which may be a molecular weight standard, a diluent, a detergent, or a labeling reagent, is loaded from a reservoir integral to the microfluidic device via a reagent introduction channel within the device. The sample and reagent form a mixture in the sample loading channel. A portion of the mixture is electrokinetically injected from the sample loading channel, via an injection channel, into a separation channel, where it is separated electrophoretically.

摘要翻译： 用于将样品材料分离成不同级分的方法和系统，其使用基于压力的流体流将样品和试剂同时装载到微流体装置的样品加载通道中。 样品通过附带的外部取样毛细管从外部来源加载。 可以是分子量标准的试剂，稀释剂，洗涤剂或标记试剂，通过装置内的试剂引入通道从集成在微流体装置中的储存器装载。 样品和试剂在样品加载通道中形成混合物。 将混合物的一部分从样品加载通道经由注射通道电动注射到分离通道中，其中电泳分离。

公开(公告)号：US20050153459A1

公开(公告)日：2005-07-14

申请号：US11005331

申请日：2004-12-06

申请人： Anne Kopf-Sill ,  Steven Sundberg ,  Andrea Chow ,  Claudia Poglitsch

发明人： Anne Kopf-Sill ,  Steven Sundberg ,  Andrea Chow ,  Claudia Poglitsch

IPC分类号： B01F13/00 ,  B01L3/00 ,  G01N1/38 ,  G01N27/447 ,  G01N1/00

CPC分类号： B01L3/5027 ,  B01F13/0059 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/049 ,  B01L2400/084 ,  G01N1/38 ,  G01N27/44791 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25625 ,  Y10T436/2575

摘要： Flow rates in a microfluidic device are modulated after performing serial dilutions by flow reduction channels that draw fluid from the main channel, thus reducing the flow rate. The reduction in flow rate and/or use of smaller dimension channels allow reduced reagent consumption. In addition, multiple flow reduction channels are used for multiple concentration measurements and for performing multiple assays simultaneously on a single sample. Also included are microfluidic devices and integrated systems for performing assays using serial dilutions, single pressure sources, multiple concentration measurements, and reduced reagent consumption. Devices comprising flow reduction channels are also used to suppress pressure perturbations from spontaneous injection.

公开(公告)号：US20050202470A1

公开(公告)日：2005-09-15

申请号：US11032749

申请日：2005-01-11

申请人： Steven Sundberg ,  Michael Knapp

发明人： Steven Sundberg ,  Michael Knapp

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q2527/107 ,  C12Q2565/629

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing binding assays through construction of molecular melt curves. In particular, the devices and methods of the invention are useful in screening large numbers of different test molecules for their binding ability to target molecules.

摘要翻译： 本发明提供了用于通过构建分子熔融曲线进行结合测定的新型微流体装置和方法。 特别地，本发明的装置和方法可用于筛选大量不同的测试分子以获得靶向分子的结合能力。

公开(公告)号：US20050084612A1

公开(公告)日：2005-04-21

申请号：US11005456

申请日：2004-12-06

申请人： Hua Yang ,  Steven Sundberg

发明人： Hua Yang ,  Steven Sundberg

IPC分类号： A61L31/10 ,  B05D5/00

CPC分类号： A61L31/10 ,  Y10T428/24562 ,  Y10T428/24661 ,  Y10T428/24744 ,  Y10T428/261 ,  Y10T428/31612 ,  Y10T428/31663 ,  C08L79/02

摘要： Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.

摘要翻译： 提供了用于微流体装置的亲水性蛋白质吸附抗性涂层。 另外，提供了包括涂层的微流体装置和制造微流体装置的方法。

公开(公告)号：US20070102297A1

公开(公告)日：2007-05-10

申请号：US11617800

申请日：2006-12-29

申请人： Steven Sundberg ,  J. Parce ,  Calvin Chow

发明人： Steven Sundberg ,  J. Parce ,  Calvin Chow

IPC分类号： G01N27/00

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US20070102296A1

公开(公告)日：2007-05-10

申请号：US11617799

申请日：2006-12-29

申请人： Steven Sundberg ,  J. Parce ,  Calvin Chow

发明人： Steven Sundberg ,  J. Parce ,  Calvin Chow

IPC分类号： G01N27/00

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

摘要翻译： 通过使用完全延伸通过微流体衬底的端口来促进流体引入。 可以使用毛细管力将流体保持在端口内，并且可以通过单个端口引入一系列样品或其它流体，通过依次将流体通过基底吹出并用替代流体代替去除的流体，或通过置换 流体部分附加流体。 在另一方面，微流体衬底具有在横截面尺寸上变化的通道，使得毛细管作用仅在通道网络的有限部分内扩散流体。 在另一方面，引入端口可以包括从端口引导到流体通道的多个非常小的通道，以便过滤除了通道和引入端口之间的连接处可能阻挡通道的颗粒或其它污染物 。

公开(公告)号：US20060286000A1

公开(公告)日：2006-12-21

申请号：US11493996

申请日：2006-07-27

申请人： Steven Sundberg ,  J. Parce ,  Calvin Chow

发明人： Steven Sundberg ,  J. Parce ,  Calvin Chow

IPC分类号： B01L3/02

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US06468761B2

公开(公告)日：2002-10-22

申请号：US09755608

申请日：2001-01-05

申请人： Hua Yang ,  Steven Sundberg

发明人： Hua Yang ,  Steven Sundberg

IPC分类号： C12Q137

CPC分类号： B01L3/5027 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/37 ,  G01N33/6809

摘要： Enzyme assays are performed in microfluidic devices including, e.g., in-line labeling, separation, and detection of assay products. In-line labeling allows assays, e.g., protease assays, to be performed in a continuous flow microfluidic format. Also included are microfluidic devices and integrated systems for performing in-line labeling in continuous flow enzyme assays.

摘要翻译： 在微流体装置中进行酶测定，包括例如在线标记，分离和检测测定产物。 在线标记允许以连续流微流体形式进行测定，例如蛋白酶测定。 还包括用于在连续流动酶测定中进行在线标记的微流体装置和集成系统。

公开(公告)号：US20070102295A1

公开(公告)日：2007-05-10

申请号：US11617804

申请日：2006-12-29

申请人： Steven Sundberg ,  J. Parce ,  Calvin Chow

发明人： Steven Sundberg ,  J. Parce ,  Calvin Chow

IPC分类号： C07K1/26

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US20070092400A1

公开(公告)日：2007-04-26

申请号：US11617795

申请日：2006-12-29

申请人： Steven Sundberg ,  J. Parce ,  Calvin Chow

发明人： Steven Sundberg ,  J. Parce ,  Calvin Chow

IPC分类号： G01N21/00

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.