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18 条记录 (耗时 0.024 秒)

    Selective Detection of Proteins that Contain Two or More Alpha-Helical Transmembrane Domains
    1.
    发明申请
    Selective Detection of Proteins that Contain Two or More Alpha-Helical Transmembrane Domains 审中-公开
    含有两个或更多个α-螺旋跨膜结构域的蛋白质的选择性检测

    公开(公告)号：US20100267078A1

    公开(公告)日：2010-10-21

    申请号：US12767972

    申请日：2010-04-27

    申请人： Wayne F. Patton ,  Courtenay R. Hart

    发明人： Wayne F. Patton ,  Courtenay R. Hart

    IPC分类号： C12Q1/02

    CPC分类号： C07K1/26

    摘要： Embodiments of the present invention provide a staining solution and of method of using the staining solution for selectively detecting proteins that contain two or more α-helical transmembrane domains. The staining solution comprises a lipophilic dyes and at least about a 30% hydrophobic solvent. The dyes of the present are represented by the general formula A-B-E wherein A is a nitrogen heterocycle, B is a bridge moiety and E is an electron pair accepting moiety that comprises either a carbonyl or nitrogen atom. In one embodiment these lipophilic dyes are merocyanine dye, a cyanine dye, a styryl dye or a carbazolylvinyl dye.

    摘要翻译： 本发明的实施方案提供了染色溶液和使用该染色溶液选择性检测含有两个或更多个α-螺旋跨膜结构域的蛋白质的方法。 染色溶液包含亲脂性染料和至少约30％的疏水性溶剂。 本发明的染料由通式A-B-E表示，其中A是氮杂环，B是桥部分，E是包含羰基或氮原子的电子对接受部分。 在一个实施方案中，这些亲脂性染料是部花菁染料，花青染料，苯乙烯基染料或咔唑基乙烯基染料。

    Detecting phospho-transfer activity
    2.
    发明授权
    Detecting phospho-transfer activity 失效
    检测磷酸转移活性

    公开(公告)号：US07550271B2

    公开(公告)日：2009-06-23

    申请号：US11742443

    申请日：2007-04-30

    申请人： Wayne F. Patton ,  Thomas Edwin Miller

    发明人： Wayne F. Patton ,  Thomas Edwin Miller

    IPC分类号： C12Q1/48

    CPC分类号： C12Q1/485 ,  C12Q1/42

    摘要： Disclosed are methods and reagents for detecting the presence of and/or activity of one or more phospho-transfer activities such as kinase, phosphatase, nucleotide cyclase, or phosphodiesterase activities.

    摘要翻译： 公开了用于检测一种或多种磷酸转移活性如激酶，磷酸酶，核苷酸环化酶或磷酸二酯酶活性的存在和/或活性的方法和试剂。

    Methods for target molecule detection using siderophores and related compositions
    6.
    发明授权
    Methods for target molecule detection using siderophores and related compositions 有权
    使用铁载体和相关组合物的靶分子检测方法

    公开(公告)号：US08859291B2

    公开(公告)日：2014-10-14

    申请号：US11148478

    申请日：2005-06-09

    申请人： Roger Bossé ,  Wayne F. Patton ,  Philippe Roby

    发明人： Roger Bossé ,  Wayne F. Patton ,  Philippe Roby

    IPC分类号： G01N33/00 ,  G01N25/18 ,  G01N27/00 ,  G01N21/62 ,  G01N21/76 ,  G01N24/00

    CPC分类号： G01N33/6842 ,  Y10T436/16 ,  Y10T436/163333 ,  Y10T436/166666 ,  Y10T436/17 ,  Y10T436/170769 ,  Y10T436/176152 ,  Y10T436/176921 ,  Y10T436/18 ,  Y10T436/182 ,  Y10T436/188 ,  Y10T436/24

    摘要： The invention provides methods for isolating a target molecule from a sample. In an embodiment, the method involves contacting a sample with a capture agent, the agent comprising a siderophore and a transition metal cation, under conditions wherein the agent is capable of binding a target molecule to form a target molecule-capture agent complex, wherein the target molecule is selected from the group consisting of a phosphorylated molecule, a nitrotyrosine-containing molecule and a sulfated molecule, and separating the target molecule-capture agent complex from the sample, thereby isolating the target molecule from the sample. Also provided are methods for determining the presence of a target molecule in a sample, that involve contacting a sample with a capture agent, the agent comprising a siderophore and a transition metal cation.

    摘要翻译： 本发明提供了从样品中分离靶分子的方法。 在一个实施方案中，所述方法包括在其中所述试剂能够结合靶分子以形成靶分子捕获剂复合物的条件下使样品与捕获剂接触，所述捕获剂包含铁载体和过渡金属阳离子，其中 靶分子选自磷酸化分子，含硝基酪氨酸的分子和硫酸化分子，并从样品中分离靶分子捕获剂复合物，从而从样品中分离靶分子。 还提供了确定样品中靶分子存在的方法，其涉及使样品与捕获剂接触，所述试剂包含铁载体和过渡金属阳离子。

    Reagents for labeling biomolecules having aldehyde or ketone moieties
    7.
    发明授权
    Reagents for labeling biomolecules having aldehyde or ketone moieties 有权
    用于标记具有醛或酮部分的生物分子的试剂

    公开(公告)号：US06967251B2

    公开(公告)日：2005-11-22

    申请号：US09970215

    申请日：2001-10-02

    申请人： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

    发明人： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

    IPC分类号： G01N33/532 ,  C07D239/91 ,  C07D239/94 ,  C07D249/08 ,  C07D263/60 ,  C07D265/22 ,  C07D277/66 ,  C07D401/04 ,  G01N33/58 ,  C12Q1/68 ,  G01N1/30

    CPC分类号： C07D265/22 ,  C07D239/91 ,  Y10T436/143333 ,  Y10T436/200833

    摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.

    摘要翻译： 描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。 在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R 5是-L-Z; L是亚芳基或C 1-6个全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R 11 -R 14独立地为H，C 1-6烷基，C 1-6烷氧基，C 全氟烷基，C 1-6烷基氨基，二（C 2-12烷基）氨基，氨基，羧基，氰基，卤素，C 1-6烷基氨基， 羟基，硝基，苯基或磺基; R 21和R 24独立地是H，C 1-6烷基，C 1-6烷氧基， C 1-6烷基全氟烷基，C 1-6烷基氨基，二（C 2-12-12 - 烷基）氨基，氨基，羧基，氰基， 卤素，羟基，硝基，苯基，磺基或-LZ。 描述了用衍生化试剂处理样品的方法。 这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

    Assay particles and methods of use
    9.
    发明授权
    Assay particles and methods of use 有权
    测定颗粒和使用方法

    公开(公告)号：US09562895B2

    公开(公告)日：2017-02-07

    申请号：US12689730

    申请日：2010-01-19

    申请人： Wayne F. Patton

    发明人： Wayne F. Patton

    IPC分类号： G01N33/543 ,  C12Q1/42 ,  C12Q1/48 ,  G01N33/542 ,  G01N33/573

    CPC分类号： G01N33/54313 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/542 ,  G01N33/54306 ,  G01N33/5432 ,  G01N33/5436 ,  G01N33/573 ,  G01N2333/9121 ,  G01N2333/916

    摘要： The invention provides assay particles useful, for example, for detecting analytes and binding molecule interactions. One type of assay particle includes a core portion encased by a shell portion, wherein the shell portion comprises an inorganic phosphor that binds selectively to a target molecule. Another type of an assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the coat portion comprises an inorganic phosphor that binds selectively to a target molecule. A further type of assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the coat portion comprises an inorganic phosphor and a target selective binding moiety, and wherein the assay particle is buoyant in aqueous media. An additional type of assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the shell portion comprises an inorganic phosphor and the coat portion comprises a target selective binding moiety, and wherein the assay particle is buoyant in aqueous media. Also provided are kits and related methods.

    摘要翻译： 本发明提供了可用于例如用于检测分析物和结合分子相互作用的测定颗粒。 一种测定颗粒包括由壳部分包围的芯部分，其中壳部分包含与靶分子选择性结合的无机磷光体。 另一类型的测定颗粒包括由壳部分包围的芯部分和覆盖壳部分的涂层部分，其中涂层部分包含与靶分子选择性结合的无机磷光体。 另一类型的测定颗粒包括由壳部分包围的芯部分和覆盖壳部分的涂层部分，其中涂层部分包含无机磷光体和靶选择性结合部分，并且其中测定颗粒在水性介质中是浮力的 。 附加类型的测定颗粒包括由壳部分包围的芯部分和覆盖壳部分的涂层部分，其中壳部分包含无机荧光体，并且涂层部分包含靶选择性结合部分，并且其中测定颗粒为 漂浮在含水介质中。 还提供了套件和相关方法。

    Quinazoline-containing kits for labeling aldehyde or ketone moieties
    10.
    发明授权
    Quinazoline-containing kits for labeling aldehyde or ketone moieties 有权
    用于标记醛或酮部分的含喹唑啉试剂盒

    公开(公告)号：US07816527B2

    公开(公告)日：2010-10-19

    申请号：US11182122

    申请日：2005-07-15

    申请人： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

    发明人： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

    IPC分类号： C07D239/72

    CPC分类号： C07D265/22 ,  C07D239/91 ,  Y10T436/143333 ,  Y10T436/200833

    摘要： Kits including novel fluorescent derivitization reagents for labeling an aldehyde- and/or ketone-containing target substance in a sample are described. In one embodiment kits are provided the novel fluorescent derivitization reagents of which have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-16 alkyl, C1-6 alkylamino, amino, or halogen; and R21-R24 are independently H, C 1-6 alkyl, C 1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The kits and reagents described are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.

    摘要翻译： 描述了包括用于标记样品中含醛和/或酮的目标物质的新型荧光衍生试剂的试剂盒。 在一个实施方案中提供试剂盒，其新型荧光衍生试剂具有下式：其中Q为羰基，硫代羰基或磺酰基，R5为-L-Z; L是亚芳基或C1-6全氟亚烷基; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R11-R14独立地为H，C1-16烷基，C1-6烷基氨基，氨基或卤素; R 21 -R 24独立地为H，C 1-6烷基，C 1-6烷氧基，C 1-6全氟烷基，C 1-6烷基氨基，二（C 2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基， 硝基，苯基，磺基或-LZ。 所述的试剂盒和试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。