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    Multiple component nanoparticles for multiplexed signaling and optical encoding
    1.
    发明授权
    Multiple component nanoparticles for multiplexed signaling and optical encoding 有权
    用于复用信令和光编码的多组分纳米颗粒

    公开(公告)号：US08168447B2

    公开(公告)日：2012-05-01

    申请号：US12066308

    申请日：2006-10-10

    申请人： Weihong Tan ,  Lin Wang ,  Chaoyong Yang

    发明人： Weihong Tan ,  Lin Wang ,  Chaoyong Yang

    IPC分类号： G01N33/552

    CPC分类号： G01N33/582 ,  G01N33/5432 ,  G01N33/587 ,  Y10T428/2982

    摘要： The present invention provides multiple-luminophore silica nanoparticles for multiplexed signaling in bioanalysis. In specific embodiments, two inorganic luminophores, Tris(2,2′-bipyridyl)osmium(II) bis(hexafluorophosphate) (OsBpy) and Tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy), or three organic luminophores 5-Fluorescein isothiocyanate (5-FITC), 5-carboxyrhodamine 6G, succinimidyl ester (5-CR6G, SE), 6-carboxy-X-rhodamine, succinimidyl ester (6-ROX, SE) can be simultaneously entrapped inside silica nanoparticles at controlled ratios, with desirable sizes and required surface functionality. Single-wavelength excitation with multiple emission endows the nanoparticles with optical encoding capability for rapid and high-throughput multiplexed detection.

    摘要翻译： 本发明提供用于生物分析中多重信号传导的多发光二氧化硅纳米颗粒。 在具体实施方案中，两种无机发光体，三（2,2'-联吡啶）锇（II）双（六氟磷酸盐）（OsBpy）和三（2,2'-联吡啶）六水合二钌（II）（RuBpy）或三种有机 发光体5-氟代异硫氰酸酯（5-FITC），5-羧基罗丹胺6G，琥珀酰亚胺酯（5-CR6G，SE），6-羧基-X-罗丹明，琥珀酰亚胺酯（6-ROX，SE）可以同时包埋在二氧化硅纳米颗粒内 具有所需的尺寸和所需的表面功能。 具有多次发射的单波长激发使纳米颗粒具有光学编码能力，用于快速和高通量多路复用检测。

    Multiple Component Nanoparticles for Multiplexed Signaling and Optical Encoding
    2.
    发明申请
    Multiple Component Nanoparticles for Multiplexed Signaling and Optical Encoding 有权
    用于复用信令和光编码的多组分纳米颗粒

    公开(公告)号：US20090017476A1

    公开(公告)日：2009-01-15

    申请号：US12066308

    申请日：2006-10-10

    申请人： Weihong Tan ,  Lin Wang ,  Chaoyong Yang

    发明人： Weihong Tan ,  Lin Wang ,  Chaoyong Yang

    IPC分类号： G01N33/53 ,  B32B1/00

    CPC分类号： G01N33/582 ,  G01N33/5432 ,  G01N33/587 ,  Y10T428/2982

    摘要： The present invention provides multiple-luminophore silica nanoparticles for multiplexed signaling in bioanalysis. In specific embodiments, two inorganic luminophores, Tris(2,2′-bipyridyl)osmium(II) bis(hexafluorophosphate) (OsBpy) and Tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy), or three organic luminophores 5-Fluorescein isothiocyanate (5-FITC), 5-carboxyrhodamine 6G, succinimidyl ester (5-CR6G, SE), 6-carboxy-X-rhodamine, succinimidyl ester (6-ROX, SE) can be simultaneously entrapped inside silica nanoparticles at controlled ratios, with desirable sizes and required surface functionality. Single-wavelength excitation with multiple emission endows the nanoparticles with optical encoding capability for rapid and high-throughput multiplexed detection.

    摘要翻译： 本发明提供用于生物分析中多重信号传导的多发光二氧化硅纳米颗粒。 在具体实施方案中，两种无机发光体，三（2,2'-联吡啶）锇（II）双（六氟磷酸盐）（OsBpy）和三（2,2'-联吡啶）六水合二钌（II）（RuBpy）或三种有机 发光体5-氟代异硫氰酸酯（5-FITC），5-羧基罗丹胺6G，琥珀酰亚胺酯（5-CR6G，SE），6-羧基-X-罗丹明，琥珀酰亚胺酯（6-ROX，SE）可以同时包埋在二氧化硅纳米颗粒内 具有所需的尺寸和所需的表面功能。 具有多次发射的单波长激发使纳米颗粒具有光学编码能力，用于快速和高通量多路复用检测。

    MULTI-ACCEPTOR MOLECULAR PROBES AND APPLICATIONS THEREOF
    3.
    发明申请
    MULTI-ACCEPTOR MOLECULAR PROBES AND APPLICATIONS THEREOF 失效
    多受体分子探针及其应用

    公开(公告)号：US20100248385A1

    公开(公告)日：2010-09-30

    申请号：US11570491

    申请日：2005-06-17

    申请人： Weihong Tan ,  Chaoyong Yang

    发明人： Weihong Tan ,  Chaoyong Yang

    IPC分类号： G01N21/76 ,  C07H21/00

    CPC分类号： G01N33/542 ,  G01N33/5308 ,  Y10T436/143333

    摘要： An oligonucleotide-based molecular probe includes at least one pin loop, the pin loop including a loop sequence complementary to a target sequence. A first stem sequence is attached to one end of the pin loop, the first stem having at least one fluorescent label attached thereto. A second stem sequence is attached to the other end of the pin loop. The second stem has a plurality of quencher molecules attached thereto.

    摘要翻译： 基于寡核苷酸的分子探针包括至少一个引脚环，引脚环包括与靶序列互补的环序列。 第一茎序列连接到针环的一端，第一茎具有至少一个附着于其上的荧光标记。 第二个茎序列连接到针环的另一端。 第二茎具有多个与其连接的猝灭剂分子。

    Hybrid Molecular Probe
    4.
    发明申请
    Hybrid Molecular Probe 审中-公开
    杂交分子探针

    公开(公告)号：US20090156416A1

    公开(公告)日：2009-06-18

    申请号：US11994965

    申请日：2006-10-11

    申请人： Weihong Tan ,  Chaoyong Yang

    发明人： Weihong Tan ,  Chaoyong Yang

    IPC分类号： C40B30/04 ,  C07H21/00 ,  C07K14/00 ,  G01N33/48

    CPC分类号： C12Q1/6876 ,  Y10T436/143333

    摘要： A system and method for analyzing a substance, in particular RNA in vivo, comprising a hybrid molecular probe, said probe comprising two single-stranded nucleic acid sequences tethered together with a polyethylene glycol polymer and fluorophores attached to either end of the sequences. When a probe of the invention hybridizes to a target substance (such as a target RKA sequence), fluorescence resonance energy transfer occurs between the two fluorophores to generate a visible signal.

    摘要翻译： 用于分析物质，特别是体内RNA的系统和方法，其包含杂交分子探针，所述探针包含与聚乙二醇聚合物连接的两条单链核酸序列和连接于序列任一端的荧光团。 当本发明的探针与目标物质（例如目标RKA序列）杂交时，在两个荧光团之间发生荧光共振能量转移以产生可见信号。

    Multi-acceptor molecular probes and applications thereof
    5.
    发明授权
    Multi-acceptor molecular probes and applications thereof 失效
    多受体分子探针及其应用

    公开(公告)号：US08652851B2

    公开(公告)日：2014-02-18

    申请号：US11570491

    申请日：2005-06-17

    申请人： Weihong Tan ,  Chaoyong Yang

    发明人： Weihong Tan ,  Chaoyong Yang

    IPC分类号： G01N21/76

    CPC分类号： G01N33/542 ,  G01N33/5308 ,  Y10T436/143333

    摘要： An oligonucleotide-based molecular probe includes at least one pin loop, the pin loop including a loop sequence complementary to a target sequence. A first stem sequence is attached to one end of the pin loop, the first stem having at least one fluorescent label attached thereto. A second stem sequence is attached to the other end of the pin loop. The second stem has a plurality of quencher molecules attached thereto.

    摘要翻译： 基于寡核苷酸的分子探针包括至少一个引脚环，引脚环包括与靶序列互补的环序列。 第一茎序列连接到针环的一端，第一茎具有至少一个附着于其上的荧光标记。 第二个茎序列连接到针环的另一端。 第二茎具有多个与其连接的猝灭剂分子。

    Micro optical fiber light source and sensor and method of fabrication thereof
    7.
    发明授权
    Micro optical fiber light source and sensor and method of fabrication thereof 失效
    微光纤光源和传感器及其制造方法

    公开(公告)号：US5361314A

    公开(公告)日：1994-11-01

    申请号：US941879

    申请日：1992-09-04

    申请人： Raoul Kopelman ,  Weihong Tan ,  Zhong-You Shi

    发明人： Raoul Kopelman ,  Weihong Tan ,  Zhong-You Shi

    IPC分类号： G01Q60/18 ,  G02B6/24 ,  G02B6/255 ,  G02B6/36 ,  G02B6/00 ,  B05D5/06 ,  H01J5/16

    CPC分类号： G02B6/241 ,  B82Y20/00 ,  B82Y35/00 ,  G01Q60/22 ,  G02B6/2552 ,  G02B6/3624

    摘要： This invention relates generally to the development of and a method of fabricating a micro optical fiber light source. An optical fiber micro-light source is presented whose aperture is extremely small yet able to act as an intense light source. Light sources of this type have wide ranging applications, including use as micro-sensors in NSOM. Micro-sensor light sources have excellent detection limits as well as photo stability, reversibility, and millisecond response times. Furthermore, a method for manufacturing a micro optical fiber light source is provided. It involves the photo-chemical attachment of an optically active material onto the end surface of an optical fiber cable which has been pulled to form an end with an extremely narrow aperture. More specifically, photopolymerization has been applied as a means to photo-chemically attach an optically active material. This process allows significant control of the size of the micro light source. Furthermore, photo-chemically attaching an optically active material enables the implementation of the micro-light source in a variety of sensor applications.

    摘要翻译： 本发明一般涉及微型光纤光源的制造和制造方法。 提出了一种光纤微光源，其光圈非常小，但能够充当强光源。 这种光源具有广泛的应用，包括在NSOM中用作微型传感器。 微传感器光源具有优异的检测限以及光稳定性，可逆性和毫秒响应时间。 此外，提供了一种用于制造微型光纤光源的方法。 它涉及光学活性材料的光化学附着到光纤电缆的端面上，该光缆被拉动以形成具有非常窄的孔径的端部。 更具体地，已经应用光聚合作为光学化学连接光学活性材料的手段。 该过程允许显着控制微光源的尺寸。 此外，光学化学地附着光学活性材料使得能够在各种传感器应用中实现微光源。

    APTAMERS THAT BIND ABNORMAL CELLS
    9.
    发明申请
    APTAMERS THAT BIND ABNORMAL CELLS 审中-公开
    咬伤异常细胞的APTAMERS

    公开(公告)号：US20090239762A1

    公开(公告)日：2009-09-24

    申请号：US12366460

    申请日：2009-02-05

    申请人： Weihong Tan ,  Hul Chen

    发明人： Weihong Tan ,  Hul Chen

    IPC分类号： C40B30/04 ,  C07H21/04 ,  G01N33/574 ,  C12Q1/68

    CPC分类号： C07H21/04 ,  C12N15/115 ,  C12N2310/16 ,  G01N33/57415

    摘要： A new aptamer approach for the recognition of specific small cell lung cancer (SCLC) cell surface molecular markers relies on cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX) to evolve aptamers for whole live cells that express a variety of surface markers representing molecular differences among cancer cells. When applied to different lung cancer cells including those from patient samples, these aptamers bind to SCLC cells with high affinity and specificity in different assay formats. When conjugated with magnetic and fluorescent nanoparticles, the aptamer nano-conjugates could effectively extract SCLC cells from mixed cell media for isolation, enrichment, and sensitive detection.

    摘要翻译： 用于识别特异性小细胞肺癌（SCLC）细胞表面分子标记物的新的适体方法依赖于通过指数富集（细胞SELEX）的配体的基于细胞的系统演化来产生表达多种表面的整个活细胞的适体 表示癌细胞之间的分子差异的标记。 当应用于包括来自患者样品的不同肺癌细胞时，这些适体以不同测定形式的高亲和力和特异性结合SCLC细胞。 当与磁性和荧光纳米颗粒结合时，适体纳米缀合物可以有效地从混合细胞培养基中提取SCLC细胞用于分离，富集和灵敏检测。

    Photoregulated Reversible Hydrogels for Delivery and Releasing of Drugs and Other Therapeutical Reagents
    10.
    发明申请
    Photoregulated Reversible Hydrogels for Delivery and Releasing of Drugs and Other Therapeutical Reagents 审中-公开
    用于递送和释放药物和其他治疗试剂的光调节的可逆水凝胶

    公开(公告)号：US20120228520A1

    公开(公告)日：2012-09-13

    申请号：US13390754

    申请日：2010-08-12

    申请人： Weihong Tan ,  Huaizhi Kang

    发明人： Weihong Tan ,  Huaizhi Kang

    IPC分类号： G21K5/04 ,  A61K47/32

    CPC分类号： A61K9/06 ,  A61K9/0009 ,  A61K41/0028 ,  A61K47/34

    摘要： A novel hydrogel delivery systems useful for encapsulating and releasing pharmaceuticals or chemicals is disclosed where water soluble polymers containing crosslinker repeating units that associate or dissociate with complementary crosslinking repeating units or separate linkers to reversibly crosslink the hydrogel. In an exemplary embodiment, a DNA crosslinked hydrogel displays photoreversibility. An exemplary hydrogel delivery system comprises DNA polymer conjugates, wherein complementary DNA sequences are crosslinked with polymer chains and hybridization of the DNA sequences is controlled by photoresponsive moieties. Such hydrogels can be used to release drug molecules and/or other therapeutic reagents. The exemplary hydrogel employs photosensitive azobenzene moieties that are incorporated into the DNA crosslinker units. The azobenzene moieties respond to different wavelengths of light so that the state of azobenzene isomerization is induced by the proportion of visible and UV light irradiated. The isomer state of the azobenzene dictates whether the complementary DNA sequences hybridize to cross link the DNA polymer conjugates. Thus, irradiation of light (visible or UV) can transform the hydrogel network between a sol and any of multiple gel states to regulate the degree of crosslinking between complementary DNA sequences and, therefore, provide a profile of release of a hydrogel encapsulated pharmaceutical or other chemical.

    摘要翻译： 公开了一种用于包封和释放药物或化学物质的新型水凝胶递送系统，其中含有交联剂重复单元的水溶性聚合物与互补的交联重复单元或分离的连接体缔合或解离以可逆地交联水凝胶。 在示例性实施方案中，DNA交联的水凝胶显示出光可逆性。 示例性的水凝胶递送系统包含DNA聚合物缀合物，其中互补DNA序列与聚合物链交联，DNA序列的杂交由光响应部分控制。 这种水凝胶可用于释放药物分子和/或其它治疗试剂。 示例性的水凝胶使用掺入到DNA交联剂单元中的光敏偶氮苯部分。 偶氮苯部分响应于不同波长的光，使得偶氮苯异构化的状态由照射的可见光和UV光的比例引发。 偶氮苯的异构体状态指示互补DNA序列是否杂交以交联DNA聚合物缀合物。 因此，光的照射（可见或UV）可以将溶胶和多种凝胶状态中的任何一种之间的水凝胶网络转化，以调节互补DNA序列之间的交联程度，因此提供水凝胶包封的药物或其它的释放曲线 化学品。