公开(公告)号：US09841421B2

公开(公告)日：2017-12-12

申请号：US13988833

申请日：2011-11-28

申请人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

发明人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

IPC分类号： G01N33/543 ,  G01N27/82 ,  G01N27/72 ,  G01N27/74 ,  G01N35/00 ,  G01N21/27 ,  G01N21/552

CPC分类号： G01N33/54373 ,  G01N21/274 ,  G01N21/552 ,  G01N27/72 ,  G01N27/745 ,  G01N27/825 ,  G01N33/54326 ,  G01N35/0098

摘要： The invention relates to a sensor device (100) and a method for the detection of magnetic particles (1) in a sample chamber (2) with a contact surface (11). The sensor device (100) comprises a sensor unit (120, 130) for detecting magnetic particles (1) in a target region (TR) and/or in at least one reference region on the contact surface. Moreover, it comprises a magnetic field generator (140) for generating a magnetic field that shall guide magnetic particles to the contact surface. With the help of these components, an “auxiliary parameter” is determined that is related to the magnetic particles (1) and/or their movement but that is independent of binding processes taking place in the target region between magnetic particles and the contact surface. The auxiliary parameter may for example be related to the degree of mismatch between the positions reached by the magnetic particles (1) under the influence of a magnetic field and the target region (TR). The evaluation results can be used to validate and/or correct the measurements obtained in the target region (TR).

公开(公告)号：US20140057366A1

公开(公告)日：2014-02-27

申请号：US13988833

申请日：2011-11-28

申请人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus dionisius Van Zon

发明人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus dionisius Van Zon

IPC分类号： G01N33/543

CPC分类号： G01N33/54373 ,  G01N21/274 ,  G01N21/552 ,  G01N27/72 ,  G01N27/745 ,  G01N27/825 ,  G01N33/54326 ,  G01N35/0098

摘要： The invention relates to a sensor device (100) and a method for the detection of magnetic particles (1) in a sample chamber (2) with a contact surface (11). The sensor device (100) comprises a sensor unit (120, 130) for detecting magnetic particles (1) in a target region (TR) and/or in at least one reference region on the contact surface. Moreover, it comprises a magnetic field generator (140) for generating a magnetic field that shall guide magnetic particles to the contact surface. With the help of these components, an “auxiliary parameter” is determined that is related to the magnetic particles (1) and/or their movement but that is independent of binding processes taking place in the target region between magnetic particles and the contact surface. The auxiliary parameter may for example be related to the degree of mismatch between the positions reached by the magnetic particles (1) under the influence of a magnetic field and the target region (TR). The evaluation results can be used to validate and/or correct the measurements obtained in the target region (TR).

摘要翻译： 本发明涉及一种传感器装置（100）和用于在具有接触表面（11））的样品室（2）中检测磁性颗粒（1）的方法。 传感器装置（100）包括用于检测目标区域（TR）中和/或接触表面上的至少一个参考区域中的磁性颗粒（1）的传感器单元（120,130）。 此外，它包括用于产生磁场的磁场发生器（140），该磁场将磁性颗粒引导到接触表面。 在这些组件的帮助下，确定与磁性颗粒（1）和/或它们的运动相关的“辅助参数”，但是与磁性颗粒和接触表面之间的目标区域中发生的结合过程无关。 辅助参数可以例如与在磁场和目标区域（TR）的影响下由磁性颗粒（1）达到的位置之间的失配程度相关。 评估结果可用于验证和/或校正在目标区域（TR）中获得的测量结果。

公开(公告)号：US09500584B2

公开(公告)日：2016-11-22

申请号：US14125093

申请日：2012-06-25

申请人： Jacobus Hermanus Maria Neijzen ,  Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko

发明人： Jacobus Hermanus Maria Neijzen ,  Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko

IPC分类号： G01N21/00 ,  G01N21/59 ,  G01N21/03 ,  G01N21/31 ,  G01N21/552 ,  G01N33/543 ,  G01N21/17

CPC分类号： G01N21/59 ,  G01N21/0303 ,  G01N21/31 ,  G01N21/3151 ,  G01N21/552 ,  G01N33/54326 ,  G01N2021/0325 ,  G01N2021/1738 ,  G01N2021/1742 ,  G01N2201/024 ,  G01N2201/066

摘要： An apparatus for examination of a sample includes at least one sample chamber in which the sample can be provided, where the sample chamber has a detection surface; at least one light source for emitting a first input light beam which is totally internally reflected at the detection surface of the sample chamber into a first output light beam, and for emitting a second input light beam which is at least partially transmitted through the sample chamber into a second output light beam. The apparatus further includes at least one light detector for detecting the first and the second output light beams. The sample chamber is elongated and traversed in longitudinal direction by light of the second input light beam.

摘要翻译： 用于检查样品的装置包括至少一个样品室，其中可以提供样品，其中样品室具有检测表面; 至少一个光源，用于发射在样品室的检测表面处完全内部反射到第一输出光束中的第一输入光束，并且用于发射至少部分透射通过样品室的第二输入光束 进入第二输出光束。 该装置还包括至少一个用于检测第一和第二输出光束的光检测器。 样品室通过第二输入光束的光线在纵向方向上被细长并横越。

公开(公告)号：US20140118745A1

公开(公告)日：2014-05-01

申请号：US14125093

申请日：2012-06-25

申请人： Jacobus Hermanus Maria Neijzen ,  Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko

发明人： Jacobus Hermanus Maria Neijzen ,  Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko

IPC分类号： G01N21/59

CPC分类号： G01N21/59 ,  G01N21/0303 ,  G01N21/31 ,  G01N21/3151 ,  G01N21/552 ,  G01N33/54326 ,  G01N2021/0325 ,  G01N2021/1738 ,  G01N2021/1742 ,  G01N2201/024 ,  G01N2201/066

摘要： The invention relates to means for the examination of a sample, wherein a first input light beam (L1) is totally internally reflected at a detection surface of a sample chamber (111), while a second input light beam (L1′) is transmitted through the sample chamber (111). The resulting first and second output light beams (L2, L2′) are detected and can be evaluated with respect to frustrated total internal reflection and optical absorbance, respectively. Preferably, both output light beams (L2, L2′) are detected by a single image sensor (155).

摘要翻译： 本发明涉及一种用于检查样品的装置，其中第一输入光束（L1）在样品室（111）的检测表面处被全内反射，而第二输入光束（L1'）被透射 样品室（111）。 检测所得到的第一和第二输出光束（L2，L2'），并且可以分别对沮丧的全内反射和光吸收率进行评估。 优选地，两个输出光束（L2，L2'）由单个图像传感器（155）检测。

公开(公告)号：US09720003B2

公开(公告)日：2017-08-01

申请号：US13141439

申请日：2009-12-15

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

IPC分类号： G01N33/68 ,  G01N33/543

CPC分类号： G01N33/6887 ,  G01N33/54373 ,  G01N2800/324

摘要： The present invention relates to a method for measuring Troponin I in a sample comprising the steps of providing a sample, contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label, contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface and detecting the magnetic label on the sensor surface. The invention further relates to a device and a cartridge for measuring Troponin I in a sample.

公开(公告)号：US09023651B2

公开(公告)日：2015-05-05

申请号：US13124407

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

发明人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

IPC分类号： G01N33/553 ,  G01N33/543 ,  B82Y25/00 ,  G01N27/74 ,  G01R33/09 ,  G01R33/12 ,  G01N35/00

CPC分类号： G01N33/54326 ,  B82Y25/00 ,  G01N27/745 ,  G01N35/0098 ,  G01R33/093 ,  G01R33/1269

摘要： The invention relates to a method and a device (100) for determining the amount of a target component (2) in a sample, wherein magnetic particles (2) can bind to a contact surface (4) with kinetics that depend on the sample-amount of the target component. The method comprises at least two washing steps during which magnetic particles (2) are magnetically moved away from the contact surface (4) and corresponding measurements of the remaining amount of magnetic particles (2) at the contact surface (4). The amount of target component in the sample is estimated from at least one of such measurement results. The measurement allows to determine also high concentrations of target component for which the sensor surface (4) is saturated in a steady-state.

摘要翻译： 本发明涉及一种用于确定样品中目标组分（2）的量的方法和装置（100），其中磁性颗粒（2）可以结合取决于样品的动力学的接触表面（4） 目标成分的数量。 该方法包括至少两个洗涤步骤，在此过程中，磁性颗粒（2）被磁性地远离接触表面（4）移动，并且在接触表面（4）处磁性颗粒（2）剩余量的相应测量值。 从这些测量结果中的至少一个估计样品中的目标组分的量。 该测量允许确定传感器表面（4）在稳定状态下饱和的目标部件的高浓度。

公开(公告)号：US20120171781A1

公开(公告)日：2012-07-05

申请号：US13496023

申请日：2010-09-07

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N33/566 ,  G01N21/75 ,  G01N33/577

CPC分类号： G01N33/54346 ,  G01N33/54333 ,  G01N2333/58

摘要： The present invention is related to an immunoassay for the detection of an analyte in a sample, said assay comprising a plurality of moieties capable of binding to said analyte, wherein capture moieties which are not specific for the same epitope are bound to a solid substrate, and at least one epitope-specific detection moiety is bound to a detectable marker, and wherein the detectable marker to which the epitope-specific detection moiety is bound is a large particle marker having a particle size of ≧50 nm and ≦5000 nm.

摘要翻译： 本发明涉及用于检测样品中分析物的免疫测定法，所述测定法包括能够结合所述分析物的多个部分，其中对相同表位不特异的捕获部分结合到固体底物上， 并且至少一个表位特异性检测部分结合到可检测标记上，并且其中表位特异性检测部分结合的可检测标记是具有≥50nm和＆lt; NE; 5000nm的粒度的大颗粒标记物。

公开(公告)号：US20110207229A1

公开(公告)日：2011-08-25

申请号：US13124407

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

发明人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

IPC分类号： G01N33/00

CPC分类号： G01N33/54326 ,  B82Y25/00 ,  G01N27/745 ,  G01N35/0098 ,  G01R33/093 ,  G01R33/1269

摘要： The invention relates to a method and a device (100) for determining the amount of a target component (2) in a sample, wherein magnetic particles (2) can bind to a contact surface (4) with kinetics that depend on the sample-amount of the target component. The method comprises at least two washing steps during which magnetic particles (2) are magnetically moved away from the contact surface (4) and corresponding measurements of the remaining amount of magnetic particles (2) at the contact surface (4). The amount of target component in the sample is estimated from at least one of such measurement results. The measurement allows to determine also high concentrations of target component for which the sensor surface (4) is saturated in a steady-state.

摘要翻译： 本发明涉及一种用于确定样品中目标组分（2）的量的方法和装置（100），其中磁性颗粒（2）可以结合取决于样品的动力学的接触表面（4） 目标成分的数量。 该方法包括至少两个洗涤步骤，在此过程中，磁性颗粒（2）被磁性地远离接触表面（4）移动，并且在接触表面（4）处磁性颗粒（2）剩余量的相应测量值。 从这些测量结果中的至少一个估计样品中的目标组分的量。 该测量允许确定传感器表面（4）在稳定状态下饱和的目标部件的高浓度。

公开(公告)号：US11493507B2

公开(公告)日：2022-11-08

申请号：US13496023

申请日：2010-09-07

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N33/543

摘要： An immunoassay for the detection of an analyte in a sample includes a plurality of moieties capable of binding to the analyte. Capture moieties, which are not specific for the same epitope, are bound to a solid substrate, and at least one epitope-specific detection moiety is bound to a detectable marker. The detectable marker is a large particle marker having a particle size of ≥50 nm and ≤5000 nm.

公开(公告)号：US20120258553A1

公开(公告)日：2012-10-11

申请号：US13518428

申请日：2010-12-16

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  David Walterus Cornelis Dekkers ,  Marco Hendrikus Hefti ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  David Walterus Cornelis Dekkers ,  Marco Hendrikus Hefti ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N27/72

CPC分类号： G01N33/54333 ,  G01N21/552 ,  G01N27/745 ,  G01N33/54393 ,  G01N2446/00

摘要： An apparatus for measuring a target molecule in a sample is disclosed. The apparatus comprises a moiety comprising a magnetic label (1) greater than 100 nm, a binding surface (12) for specifically binding the moiety, the amount of said moiety binding to said surface being indicative of the amount of the target molecule in said sample, detection means (31 31′) for detecting the amount of said moiety bound to said surface, and a salt (51) for reducing aggregation of the magnetic labels of respective moieties in said sample. The apparatus preferably also comprises a magnetic field generator (41) for attracting the magnetic labels to the binding surface. A method for measuring a target molecule in a sample and a disposable cartridge for use with the apparatus are also disclosed.

摘要翻译： 公开了一种用于测量样品中靶分子的装置。 该装置包括包含大于100nm的磁性标签（1）的部分，用于特异性结合该部分的结合表面（12），所述部分结合所述表面的量指示所述样品中靶分子的量 ，用于检测与所述表面结合的所述部分的量的检测装置（31 31'）和用于减少所述样品中各部分的磁性标记物的聚集的盐（51）。 该装置优选地还包括用于将磁性标签吸引到装订表面的磁场发生器（41）。 还公开了一种用于测量样品中的靶分子的方法和用于该设备的一次性盒。