公开(公告)号：US20060025752A1

公开(公告)日：2006-02-02

申请号：US11191676

申请日：2005-07-28

申请人： William Broaddus ,  Zhi-Jian Chen ,  George Gillies

发明人： William Broaddus ,  Zhi-Jian Chen ,  George Gillies

IPC分类号： A61M25/16

CPC分类号： A61M25/0026 ,  A61M5/007 ,  A61M25/00 ,  A61M25/0045 ,  A61M25/007 ,  A61M27/006 ,  A61M2025/1013

摘要： A method, technique and system is disclosed for the delivery of therapeutic agents and/or into the bulk brain tissues and other parts, tissues and organs of the body, including vasculature. A novel form of coaxial catheter provides a means for implanting an outer tube into the brain, then inserting an inner tube into the outer tube and aligning them such that port holes on both of the tubes will overlap and permit a flux of the therapeutic agent to flow into the brain in such a way as to minimize the effects of trapped air, virtually eliminate backflow of the agent, and avoid the potential for additional damage to the brain since only one surgical placement of the outer tube is needed. Similarly, the method, technique system may be utilized to remove fluids or other medium from the brain, tissues, and organs to minimize the effects of escaped air or negative pressure.

摘要翻译： 公开了用于递送治疗剂和/或进入体内的大脑组织和身体其他部位，组织和器官的方法，技术和系统，包括脉管系统。 同轴导管的新颖形式提供了一种用于将外管植入大脑中的装置，然后将内管插入外管并对准它们，使得两个管上的孔口将重叠并允许治疗剂的通量 流入大脑，以尽可能减少被捕获的空气的影响，实际上消除了药剂的回流，并避免了额外的损害大脑的潜力，因为只需要一个手术放置外管。 类似地，该方法技术系统可用于从脑，组织和器官中除去流体或其它介质，以最小化逃逸空气或负压的影响。

公开(公告)号：US08728053B2

公开(公告)日：2014-05-20

申请号：US13540348

申请日：2012-07-02

申请人： William Broaddus ,  Zhi-Jian Chen ,  George Gillies

发明人： William Broaddus ,  Zhi-Jian Chen ,  George Gillies

IPC分类号： A61M31/00

CPC分类号： A61M25/0026 ,  A61M5/007 ,  A61M25/00 ,  A61M25/0045 ,  A61M25/007 ,  A61M27/006 ,  A61M2025/1013

摘要： A method, technique and system is disclosed for the delivery of therapeutic agents and/or into the bulk brain tissues and other parts, tissues and organs of the body, including vasculature. A novel form of coaxial catheter provides a means for implanting an outer tube into the brain, then inserting an inner tube into the outer tube and aligning them such that port holes on both of the tubes will overlap and permit a flux of the therapeutic agent to flow into the brain in such a way as to minimize the effects of trapped air, virtually eliminate backflow of the agent, and avoid the potential for additional damage to the brain since only one surgical placement of the outer tube is needed. Similarly, the method, technique system may be utilized to remove fluids or other medium from the brain, tissues, and organs to minimize the effects of escaped air or negative pressure.

摘要翻译： 公开了用于递送治疗剂和/或进入体内的大脑组织和身体其他部位，组织和器官的方法，技术和系统，包括脉管系统。 同轴导管的新颖形式提供了一种用于将外管植入大脑中的装置，然后将内管插入外管并对准它们，使得两个管上的孔口将重叠并允许治疗剂的通量 流入大脑，以尽可能减少被捕获的空气的影响，实际上消除了药剂的回流，并避免了额外的损害大脑的潜力，因为只需要一个手术放置外管。 类似地，该方法技术系统可用于从脑，组织和器官中除去流体或其它介质，以最小化逃逸空气或负压的影响。

公开(公告)号：US20050119556A1

公开(公告)日：2005-06-02

申请号：US10985340

申请日：2004-11-10

申请人： George Gillies ,  Roger Hastings ,  Jeffrey Garibaldi ,  William Broaddus

发明人： George Gillies ,  Roger Hastings ,  Jeffrey Garibaldi ,  William Broaddus

IPC分类号： A61B5/055 ,  A61B5/06 ,  A61B19/00 ,  A61M25/01 ,  A61M25/095 ,  G01R33/28 ,  A61B5/05

CPC分类号： A61B5/055 ,  A61B5/06 ,  A61B34/73 ,  A61B2034/732 ,  A61M25/01 ,  G01R33/285

摘要： A method of magnetically manipulating a medical device within a body part of a human patient in conjunction with MR imaging includes applying a navigating magnetic field with magnets from the MR imaging device, and changing the magnetic moment of the medical device to change the orientation of the medical device within the body part

摘要翻译： 结合MR成像的磁性操纵人类患者的身体部位内的医疗装置的方法包括使用来自MR成像装置的磁体应用导航磁场，以及改变医疗装置的磁矩以改变医疗装置的取向 身体部位内的医疗器械

公开(公告)号：US06277571B1

公开(公告)日：2001-08-21

申请号：US09163485

申请日：1998-09-30

申请人： Helen Fillmore ,  William Broaddus ,  George Gillies

发明人： Helen Fillmore ,  William Broaddus ,  George Gillies

IPC分类号： C12Q168

CPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q2549/119 ,  C12Q2525/15

摘要： A method of sequential consensus region-directed amplification comprising, (a) amplifying a first segment of at least one target DNA in a DNA mixture, using a first and second oligonucleotide primer, each of which hybridizes to the target DNA, and a nucleic acid polymerase, under conditions in which DNA amplification is achieved, resulting in a first segment of double-stranded DNA; (b) amplifying a second segment of the first segment of double-stranded DNA, using a third and fourth oligonucleotide primer, each of which hybridizes to the first DNA segment, and a nucleic acid polymerase, under conditions in which DNA amplification is achieved, resulting in a second segment of double-stranded DNA.

摘要翻译： 一种连续共有区域定向扩增的方法，其包括：（a）使用与目标DNA杂交的第一和第二寡核苷酸引物和核酸（a）扩增DNA混合物中的至少一种靶DNA的第一片段 在实现DNA扩增的条件下，产生第一段双链DNA; （b）在实现DNA扩增的条件下，使用与第一DNA片段杂交的第三和第四寡核苷酸引物和核酸聚合酶扩增第二片段的双链DNA， 导致第二段双链DNA。

公开(公告)号：US20050158286A1

公开(公告)日：2005-07-21

申请号：US11022805

申请日：2004-12-28

申请人： Helen Fillmore ,  Shelley Hoover ,  William Broaddus ,  George Gillies

发明人： Helen Fillmore ,  Shelley Hoover ,  William Broaddus ,  George Gillies

IPC分类号： C12N5/071 ,  A61K45/00 ,  C12N5/08

CPC分类号： C12N5/0629 ,  C12N5/0618 ,  C12N5/0626 ,  C12N2506/091 ,  C12N2510/00

摘要： Cells are generated from skin biopsies for use in cell implantation by identifying a source of skin cells that have a surface concentration of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; taking a sample of tissue from the surface area; mechanically disaggregating the tissue samples; collecting the disaggregated cells; washing the disaggregated cells; filtering the washed disaggregated cells; providing a cell suspension with filtered and washed keritinocytes and/or melanocytes; and suspending the cell suspension in a medium. In greater particularity, the method may comprise a) identifying a source of skin cells that have a surface concentration of at least 400 cells per square millimeter of surface area of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; b) taking a sample of those skin cells and culturing them to enrich for cells selected from the group consisting of keritinocytes and melanocytes to form cultured cells for transplantation purposes c) concentrating the cells selected from the group consisting of keritinocytes and/or melanocytes from the cultured mass of cells; and d) differentiating the concentrated cells selected from the group consisting of keritinocytes an/or melanocytes.

摘要翻译： 通过鉴定具有至少一种选自角质蛋白和/或黑素细胞的细胞的表面浓度的皮肤细胞来源，从皮肤活检中产生细胞，用于细胞植入; 从表面区域取样组织; 机械分解组织样品; 收集分解细胞; 洗涤分解细胞; 过滤洗涤的分解细胞; 提供具有过滤和洗涤的角质细胞和/或黑素细胞的细胞悬浮液; 并将细胞悬浮液悬浮于培养基中。 更具体地，该方法可以包括：a）鉴定表皮浓度为至少一个选自下组的角质形成细胞和/或黑素细胞的细胞每平方毫米的平均毫米表面积的皮肤细胞来源; b）取这些皮肤细胞的样品并培养它们以富集选自由角质细胞和黑素细胞组成的组的细胞，以形成用于移植目的的培养细胞; c）将选自下组的细胞和/或黑素细胞 培养细胞数量; 和d）区分选自由黑素细胞或黑素细胞组成的组的浓缩细胞。

公开(公告)号：US09218752B2

公开(公告)日：2015-12-22

申请号：US13579882

申请日：2011-02-18

申请人： George Gillies ,  Harutyun V. Gyurjyan ,  Srijoy Mahapatra ,  Jason M. Tucker-Schwartz

发明人： George Gillies ,  Harutyun V. Gyurjyan ,  Srijoy Mahapatra ,  Jason M. Tucker-Schwartz

IPC分类号： G09B23/28 ,  G09B23/30 ,  A61B18/14

CPC分类号： G09B23/303 ,  A61B18/1492 ,  G09B23/28 ,  G09B23/30

摘要： An aspect of various systems and methods provides, but not limited thereto, novel means for simulating physiological systems and processes in vitro in order to test surgical devices and train practitioners in the use of surgical devices. An aspect of various embodiments further provides in vitro anatomical components, such as a thorax, lungs, heart and pericardium, configured to contain at least one fluid having a pressure-frequency profile that may mimic typical pressure-frequency waveforms of in vivo anatomical fluids. A model communication system may be used to communicate the desired pressure-frequency profiles to the in vitro anatomical fluids. In a further aspect of various embodiments, an access device, e.g. a surgical instrument, configured to sense pressure, frequency, and/or a pressure-frequency profile may be inserted into one or more anatomical components of the in vitro model in order to test the instrument and/or train a practitioner in proper use of the instrument. An access device communication system may be used to communicate data to the practitioner. This data may include, for example, pressure-frequency data and/or the location of a portion of the access device with respect to the various in vitro anatomical components.

摘要翻译： 各种系统和方法的一个方面提供但不限于用于在体外模拟生理系统和过程的新颖手段，以便在使用外科手术装置时测试手术装置和训练从业人员。 各种实施方案的一个方面进一步提供体外解剖组分，例如胸部，肺，心脏和心包，其被配置为包含至少一种具有可以模拟体内解剖流体的典型压力 - 频率波形的压力 - 频率分布的流体。 模型通信系统可用于将期望的压力 - 频率分布传递给体外解剖流体。 在各种实施例的另一方面，访问设备，例如， 被配置为感测压力，频率和/或压力 - 频率分布的外科器械可被插入到体外模型的一个或多个解剖组件中，以便测试仪器和/或训练从业者正确使用 仪器。 访问设备通信系统可以用于向从业者传送数据。 该数据可以包括例如压力 - 频率数据和/或接入装置的一部分相对于各种体外解剖组件的位置。

公开(公告)号：US20130108999A1

公开(公告)日：2013-05-02

申请号：US13579882

申请日：2011-02-18

申请人： George Gillies ,  Harutyun V. Gyurjyan ,  Srijoy Mahapatra ,  Jason M. Tucker-Schwartz

发明人： George Gillies ,  Harutyun V. Gyurjyan ,  Srijoy Mahapatra ,  Jason M. Tucker-Schwartz

IPC分类号： G09B23/30

CPC分类号： G09B23/303 ,  A61B18/1492 ,  G09B23/28 ,  G09B23/30

摘要： An aspect of various systems and methods provides, but not limited thereto, novel means for simulating physiological systems and processes in vitro in order to test surgical devices and train practitioners in the use of surgical devices. An aspect of various embodiments further provides in vitro anatomical components, such as a thorax, lungs, heart and pericardium, configured to contain at least one fluid having a pressure-frequency profile that may mimic typical pressure-frequency waveforms of in vivo anatomical fluids. A model communication system may be used to communicate the desired pressure-frequency profiles to the in vitro anatomical fluids. In a further aspect of various embodiments, an access device, e.g. a surgical instrument, configured to sense pressure, frequency, and/or a pressure-frequency profile may be inserted into one or more anatomical components of the in vitro model in order to test the instrument and/or train a practitioner in proper use of the instrument. An access device communication system may be used to communicate data to the practitioner. This data may include, for example, pressure-frequency data and/or the location of a portion of the access device with respect to the various in vitro anatomical components.

摘要翻译： 各种系统和方法的一个方面提供但不限于用于在体外模拟生理系统和过程的新颖手段，以便在使用外科手术装置时测试手术装置和训练从业人员。 各种实施方案的一个方面进一步提供体外解剖组分，例如胸部，肺，心脏和心包，其被配置为包含至少一种具有可以模拟体内解剖流体的典型压力 - 频率波形的压力 - 频率分布的流体。 模型通信系统可用于将期望的压力 - 频率分布传递给体外解剖流体。 在各种实施例的另一方面，访问设备，例如， 被配置为感测压力，频率和/或压力 - 频率分布的外科器械可被插入到体外模型的一个或多个解剖组件中，以便测试仪器和/或训练从业者正确使用 仪器。 访问设备通信系统可以用于向从业者传送数据。 该数据可以包括例如压力 - 频率数据和/或接入装置的一部分相对于各种体外解剖组件的位置。

公开(公告)号：US20050245896A1

公开(公告)日：2005-11-03

申请号：US11105166

申请日：2005-04-13

申请人： John Kucharczyk ,  George Gillies

发明人： John Kucharczyk ,  George Gillies

IPC分类号： A61M25/00 ,  A61M31/00

CPC分类号： A61M25/003 ,  A61M25/0032 ,  A61M25/007 ,  A61M2025/0004 ,  A61M2025/0039

摘要： A method is disclosed for the delivery of therapeutic agents into tissues, blood vessels, and body ducts of the human body. A novel catheter enables controlled directing of emitted drug delivery to assist control of drug dwell time in targeted areas. One coaxial catheter embodiment provides capability for locating an outer lumen of the system into the target region, with localization of said outer lumen carried out by use of appropriate medical imaging modalities. In one embodiment, an inner lumen of the catheter means is primed with the agent to be delivered, and recirculated flow of the agent through pluralities of appropriately positioned port holes on the two lumens then occurs via one or more active and/or passive flow driving and guiding techniques intrinsic to the design of the coaxial catheter system. Another coaxial catheter embodiment carries and emits agent in an outer cannula and recaptures agent plus liquid or fluids in the body (such as blood, bile, serum, stable (non-flowing) liquid or kinetic liquid) in an inner cannula.

摘要翻译： 公开了用于将治疗剂递送到人体的组织，血管和体导管中的方法。 一种新型的导管能够控制引导排出的药物输送以帮助控制目标区域中的药物停留时间。 一个同轴导管实施例提供了将系统的外腔定位到目标区域中的能力，通过使用适当的医学成像模式对所述外腔进行定位。 在一个实施例中，导管装置的内腔用待传递的药剂引发，并且药剂的再循环流动通过两个流明上的多个适当定位的孔口然后经由一个或多个主动和/或被动流动驱动 以及设计同轴导管系统固有的指导技术。 另一个同轴导管实施例在外插管中携带并发射试剂，并在内套管中重新捕获剂体内加液体或液体（例如血液，胆汁，血清，稳定（非流动）液体或动力学液体）。

公开(公告)号：US09314265B2

公开(公告)日：2016-04-19

申请号：US13607993

申请日：2012-09-10

申请人： Srijoy Mahapatra ,  George Gillies

发明人： Srijoy Mahapatra ,  George Gillies

IPC分类号： A61B17/34 ,  A61B5/00 ,  A61B5/0215 ,  A61B5/03 ,  A61B5/20 ,  A61B17/00 ,  A61B19/00

CPC分类号： A61B17/3401 ,  A61B5/0215 ,  A61B5/031 ,  A61B5/202 ,  A61B5/4887 ,  A61B5/6848 ,  A61B5/6869 ,  A61B5/7239 ,  A61B17/34 ,  A61B17/3403 ,  A61B2017/00243 ,  A61B2090/064 ,  A61B2562/0247

摘要： A tool and method of positioning and delivering medical devices and therapeutics within the pericardial space, as well as other body part or space. A needle is inserted into the chest through a sub-xiphoid puncture, and the pressure within the needle is monitored manometrically or otherwise sensed as the needle is advanced towards the pericardial space. By reading the pressure within the needle while it is advanced, the clinician is able to know that he or she is avoiding insertion of it into organs or spaces not intended to be the target location. In addition the retractable sharp edge allows the operator to access the space and cut tissue but do so safely by retracting the sharp edge.

摘要翻译： 在心包空间以及其他身体部位或空间内定位和传送医疗装置和治疗剂的工具和方法。 针穿刺穿刺胸部插入胸部，并且当针朝着心包空间前进时，针头内的压力被监测或者以其他方式感测。 通过读取针头内的压力，临床医生能够知道他或她正在避免将其插入到不想成为目标位置的器官或空间中。 此外，可伸缩的锋利边缘允许操作者访问空间并切割组织，但是通过缩回尖锐边缘来安全地进行。

公开(公告)号：US07361506B2

公开(公告)日：2008-04-22

申请号：US11022805

申请日：2004-12-28

申请人： Helen Fillmore ,  Shelley Hoover ,  William C. Broaddus ,  George Gillies

发明人： Helen Fillmore ,  Shelley Hoover ,  William C. Broaddus ,  George Gillies

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08

CPC分类号： C12N5/0629 ,  C12N5/0618 ,  C12N5/0626 ,  C12N2506/091 ,  C12N2510/00

摘要： Cells are generated from skin biopsies for use in cell implantation by identifying a source of skin cells that have a surface concentration of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; taking a sample of tissue from the surface area; mechanically disaggregating the tissue samples; collecting the disaggregated cells; washing the disaggregated cells; filtering the washed disaggregated cells; providing a cell suspension with filtered and washed keritinocytes and/or melanocytes; and suspending the cell suspension in a medium. In greater particularity, the method may comprise a) identifying a source of skin cells that have a surface concentration of at least 400 cells per square millimeter of surface area of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; b) taking a sample of those skin cells and culturing them to enrich for cells selected from the group consisting of keritinocytes and melanocytes to form cultured cells for transplantation purposes c) concentrating the cells selected from the group consisting of keritinocytes and/or melanocytes from the cultured mass of cells; and d) differentiating the concentrated cells selected from the group consisting of keritinocytes an/or melanocytes.

摘要翻译： 通过鉴定具有至少一种选自角质蛋白和/或黑素细胞的细胞的表面浓度的皮肤细胞来源，从皮肤活检中产生细胞，用于细胞植入; 从表面区域取样组织; 机械分解组织样品; 收集分解细胞; 洗涤分解细胞; 过滤洗涤的分解细胞; 提供具有过滤和洗涤的角质细胞和/或黑素细胞的细胞悬浮液; 并将细胞悬浮液悬浮于培养基中。 更具体地，该方法可以包括：a）鉴定表皮浓度为至少一个选自下组的角质形成细胞和/或黑素细胞的细胞每平方毫米的平均毫米表面积的皮肤细胞来源; b）取这些皮肤细胞的样品并培养它们以富集选自由角质细胞和黑素细胞组成的组的细胞，以形成用于移植目的的培养细胞; c）将选自下组的细胞和/或黑素细胞 培养细胞数量; 和d）区分选自由黑素细胞或黑素细胞组成的组的浓缩细胞。