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公开(公告)号：US4052504A
公开(公告)日：1977-10-04
申请号：US701191
申请日：1976-06-30
申请人： William Hertl , Gerald Odstrchel
发明人： William Hertl , Gerald Odstrchel
CPC分类号： G01N33/78 , Y10S436/804
摘要： Method of determining the concentration of thyroxine binding globulin (TBG) in a fluid sample. The method comprises the steps of adding to the sample an excess amount of thyroxine (T.sub.4) and then analyzing the sample for T.sub.4 via immunoassay technique in the presence and absence of a blocking agent to establish a binding differential. The differential is then correlated with a standard curve which relates known TBG concentrations to binding differentials obtained in a similar manner.
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公开(公告)号：US4046870A
公开(公告)日：1977-09-06
申请号：US701192
申请日：1976-06-30
申请人： William Hertl , Gerald Odstrchel
发明人： William Hertl , Gerald Odstrchel
CPC分类号： G01N33/78
摘要： Method for determining the concentration of free (unbound) thyroid hormones (T.sub.3 or T.sub.4) in a fluid sample. The method comprises the steps of performing an immunoassay for total T.sub.3 or total T.sub.4 in the presence and absence of a blocking agent to establish a binding differential and then correlating that differential with a standard curve which relates known free thyroid hormone concentrations with binding differentials.
摘要翻译： 确定流体样品中游离（未结合）甲状腺激素（T3或T4）浓度的方法。 该方法包括以下步骤：在存在和不存在阻断剂的情况下对总T3或总T4进行免疫测定以建立结合差异，然后将该差异与将已知的游离甲状腺激素浓度与结合差异相关联的标准曲线相关联。
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公开(公告)号：US4311687A
公开(公告)日：1982-01-19
申请号：US90535
申请日：1979-11-02
申请人： William Hertl , Gerald Odstrchel
发明人： William Hertl , Gerald Odstrchel
IPC分类号： G01N33/541 , G01N33/78 , G01N33/54 , G01N33/60 , G01T1/00
CPC分类号： G01N33/78 , G01N33/541
摘要： An improved radiometric assay of dialysates from equilibrium dialysis procedures which involves using immobilized antibody specific for a given hapten to separate free hapten (including radiolabeled hapten) from radiolabeled non-hapten breakdown products.
摘要翻译： 通过平衡透析程序对透析液进行改进的辐射测定，其涉及使用特定于给定半抗原的固定化抗体将游离半抗原（包括放射性标记的半抗原）与放射性标记的非半抗原分解产物分离。
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4.发明授权
公开(公告)号：US4190535A
公开(公告)日：1980-02-26
申请号：US922825
申请日：1978-07-10
CPC分类号： G01N33/491
摘要： This invention relates to a method for isolating platelets, lymphocytes, and monocytes from anticoagulated blood. The method involves centrifuging at high force a sample of uncoagulated blood with a water-insoluble thixotropic gel-like material having a specific gravity between 1.065-1.077 g/cc for a length of time sufficient to cause the gel-like material to form a barrier between the platelets, lymphocytes, monocytes, and plasma fraction of the blood and the heavier blood cells. Thereafter, the plasma is withdrawn and the platelets, lymphocytes, and monocytes removed from above the barrier in a buffer solution. Where desired, the platelets can be separated from the lymphocytes and monocytes utilizing a similar process but wherein the water-insoluble thixotropic gel-like material has a specific gravity of less than about 1.055 g/cc.
摘要翻译： 本发明涉及从抗凝血中分离血小板，淋巴细胞和单核细胞的方法。 该方法包括以非常高的力将具有1.065-1.077g / cc之间的比重的水不溶性触变凝胶状材料的非离子的血液样品离心一段足以使凝胶状材料形成屏障的时间 在血小板，淋巴细胞，单核细胞和血液和较重血细胞的血浆级分之间。 此后，取出血浆，将血小板，淋巴细胞和单核细胞从缓冲溶液中的屏障上方除去。 如果需要，可以使用类似的方法将血小板与淋巴细胞和单核细胞分离，但其中水不溶性触变凝胶状材料的比重小于约1.055g / cc。
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公开(公告)号：US4447528A
公开(公告)日：1984-05-08
申请号：US291354
申请日：1981-08-10
IPC分类号： G01N33/564 , A61K39/00 , G01N33/566 , G01N33/82 , G01N33/58 , G01N33/50
CPC分类号： G01N33/82 , Y10S435/975 , Y10S436/804
摘要： A radioassay procedure and reagent kit therefor for detecting auto blocking antibody, such as auto blocking antibody which interferes with the complexation of intrinsic factor with vitamin B.sub.12. A receptor, i.e., intrinsic factor, is immobilized on a support and the amount of ligand, i.e., vitamin B.sub.12, capable of binding therewith in the presence of a biological fluid sample is determined.
摘要翻译： 一种用于检测自身阻断抗体的自动阻断抗体的放射性检测方法及其试剂盒，其阻碍内在因子与维生素B12的复合。 将受体（即固有因子）固定在载体上，并确定能够在生物流体样品存在下与其结合的配体（即维生素B12）的量。
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6.发明授权Determining creatine kinase isoenzmes via immobilized antibody-isoenzyme complexes 失效通过固定化抗体 - 同工酶复合物测定肌酸激酶同工酶
公开(公告)号：US4260678A
公开(公告)日：1981-04-07
申请号：US14315
申请日：1979-02-23
申请人： Cyrus A. Lepp , Gerald Odstrchel
发明人： Cyrus A. Lepp , Gerald Odstrchel
IPC分类号： G01N33/573 , C12Q1/50 , G01N33/70
CPC分类号： G01N33/70 , Y10S436/805 , Y10S436/811
摘要： Creatine kinase isoenzymes in a liquid sample are determined by means of immobilized antibody-isoenzyme complexes in which measureable isoenzyme activity is retained.
摘要翻译： 通过固定的抗体 - 同工酶复合物测定液体样品中的肌酸激酶同工酶，其中保留了可测量的同工酶活性。
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公开(公告)号：US3983000A
公开(公告)日：1976-09-28
申请号：US672633
申请日：1976-04-01
申请人： Ralph A. Messing , Gerald Odstrchel
发明人： Ralph A. Messing , Gerald Odstrchel
IPC分类号： C12N11/14 , G01N33/552 , C07G7/02
CPC分类号： C12N11/14 , G01N33/552 , Y10S435/917 , Y10S530/811
摘要： Proteins such as enzymes or antibodies can be immobilized in a biologically active state on various inorganic supports via an intermediate residue of o-dianisidine.
摘要翻译： 诸如酶或抗体的蛋白质可以通过邻联茴香胺的中间体残基固定在各种无机载体上的生物活性状态。
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公开(公告)号：US5366870A
公开(公告)日：1994-11-22
申请号：US042417
申请日：1993-04-02
申请人： Gerald Odstrchel , George Leung
发明人： Gerald Odstrchel , George Leung
CPC分类号： C12Q1/52 , Y10S435/808 , Y10S435/81
摘要： Method for determining the presence of an aminotransferase (AT.sub.1), other than aspartate aminotransferase (AST), in a biological sample. The method includes contacting the sample with an amino donor substrate for AT.sub.1, an amino acceptor substrate for AT.sub.1, cysteine sulfinic acid (CSA) in excess, a second aminotransferase (AT.sub.2), different from AT.sub.1, in excess, and an indicator for the presence of sulfite ions, under conditions in which the concentration of said AT.sub.1 can be determined by the amount of the indicator which forms a detectable signal.
摘要翻译： 确定生物样品中天冬氨酸氨基转移酶（AST）以外的氨基转移酶（AT1）的存在的方法。 该方法包括将样品与AT1的氨基供体底物，AT1的氨基受体底物，过量的半胱氨酸亚磺酸（CSA），过量的与AT1不同的第二氨基转移酶（AT2）和用于存在的指示剂 的亚硫酸根离子，其中所述AT1的浓度可以由形成可检测信号的指示剂的量确定。
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公开(公告)号：US4254218A
公开(公告)日：1981-03-03
申请号：US14294
申请日：1979-02-23
IPC分类号： C12Q1/12 , G01N33/571 , C12Q1/66 , C12N11/14
CPC分类号： C12Q1/12 , G01N33/571 , Y10S435/81
摘要： A method for detecting the presence of Neisseria bacteria in a fluid sample which comprises bringing immobilized antibody specific for the enzyme 1,2-propanediol dehydrogenase which is released from such bacteria upon lysis thereof, into contact with a lysed sample, isolating the immobilized antibody or resulting immobilized antibody-enzyme complex or mixture thereof, and then testing the isolated material for enzymatic activity.
摘要翻译： 一种用于检测流体样品中奈瑟菌属细菌存在的方法，其包括将对此类细菌释放的1,2-丙二醇脱氢酶的特异性固定化的抗体裂解，与裂解的样品接触，分离固定的抗体或 得到的固定化抗体 - 酶复合物或其混合物，然后测试分离的材料用于酶活性。
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