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公开(公告)号：US5002661A
公开(公告)日：1991-03-26
申请号：US398739
申请日：1989-08-25
申请人： William L. Chick , Susan J. Sullivan , Kermit M. Borland , John M. Harvey , Thomas E. Muller , Karen E. Dunleavy , Donald P. King, Jr. , Edward J. Doherty , Barry A. Solomon , Amy L. Foley
发明人： William L. Chick , Susan J. Sullivan , Kermit M. Borland , John M. Harvey , Thomas E. Muller , Karen E. Dunleavy , Donald P. King, Jr. , Edward J. Doherty , Barry A. Solomon , Amy L. Foley
CPC分类号： A61M1/3472 , A61F2/022 , A61L27/04 , A61L27/14 , A61M1/3475 , A61M1/1678
摘要： A device which serves as an artificial pancreas comprises a hollow fiber having an inner diameter of about 5 mm which is surrounded by islets of Langerhans enclosed in a housing. The islets are suspended in a semi-solid matrix which ensures desired distribution of the cells about the hollow fiber. The hollow fiber and suspended islets are enclosed in a housing which further aids the desired distribution of islets about the hollow fiber. The hollow fiber has a porosity which selectively allows passage of substances having a molecular weight of less than about 100,000 Daltons. The semi-solid matrix in which the islets are embedded and suspended is formed of an appropriate supporting material such as alginate or agar.
摘要翻译： 用作人造胰腺的装置包括内径约5mm的中空纤维，其被包围在壳体中的朗格汉斯岛包围。 将胰岛悬浮在半固体基质中，确保细胞围绕中空纤维的期望分布。 中空纤维和悬挂的胰岛被封闭在壳体中，这进一步有助于围绕中空纤维的胰岛的期望分布。 中空纤维具有选择性地允许具有小于约100,000道尔顿的分子量的物质通过的孔隙率。 其中胰岛被嵌入和悬浮的半固体基质由合适的支撑材料如藻酸盐或琼脂形成。
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公开(公告)号：US5912163A
公开(公告)日：1999-06-15
申请号：US953541
申请日：1997-10-20
申请人： Kermit M. Borland , Barbara A. Chandler , Dianna Hunt Picton , Shawn P. Cain , Deborah Deane , Claudy J.P. Mullon
发明人： Kermit M. Borland , Barbara A. Chandler , Dianna Hunt Picton , Shawn P. Cain , Deborah Deane , Claudy J.P. Mullon
CPC分类号： A01N1/0242 , A01N1/021 , A01N1/0247 , C12M45/09
摘要： The invention is based in part on the observation that significant portions of porcine livers appear to remain intact after perfusion by standard methods, suggesting that the perfusion procedures employed do not result in complete enzymatic digestion. Recovery of cells is therefore substantially lower than would be possible if the organs were thoroughly digested. It is found that increased perfusion flow rate, occlusion of at least one major blood vessel leading out of the organ, increased enzymatic digestion time, and vigorous tissue dissociation techniques can be combined to afford a uniquely high yield of viable cells.
摘要翻译： 本发明部分基于观察到，通过标准方法灌注后，猪肝脏的显着部分似乎保持完整，这表明所用的灌注程序不会导致完全的酶消化。 因此，如果器官被彻底消化，则细胞的恢复显着低于可能的。 发现增加的灌注流速，至少一个从器官出来的主要血管的阻塞增加了酶消化时间，并且可以组合有力的组织解离技术以提供独特的高产量的活细胞。
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公开(公告)号：US5895745A
公开(公告)日：1999-04-20
申请号：US719769
申请日：1996-09-25
CPC分类号： A01N1/0284 , A01N1/0221 , Y10T436/108331
摘要： The invention features a method of processing cryopreserved cells by thawing and equilibrating the cells at warm temperatures (e.g., between 30.degree. C. and 43.degree. C). Either the cell suspension in the cryoprotective medium is thawed to a temperature between 35.degree. C. and 43.degree. C. or the cryoprotective medium is equilibrated with a culture medium at a temperature between 35.degree. C. and 43.degree. C., or both steps are carried out at the warm temperatures. By thawing and equilibrating the cryopreserved cells at warm temperatures, the viability, (especially after 3 hours of culture), and metabolic activity (i.e., diazepam metabolism) of the cells can be improved over traditional cold cell processing (i.e., at temperatures of between 2.degree. C. and 8.degree. C.).
摘要翻译： 本发明的特征在于通过在温暖的温度（例如30℃至43℃）下解冻和平衡细胞来处理冷冻保存的细胞的方法。 将冷冻保护培养基中的细胞悬浮液解冻至35℃至43℃之间的温度，或将冷冻保护培养基在培养基中在35℃至43℃之间的温度下平衡，或两个步骤 在温暖的温度下进行。 通过在温暖的温度下融化和平衡冷冻保存的细胞，可以比传统的冷细胞处理（即，在两者之间的温度）之间改善细胞的存活力（特别是培养3小时）和代谢活性（即，地西泮代谢） 2℃和8℃）。
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