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11 条记录 (耗时 0.026 秒)

    Artificial pancreatic perfusion device with reseedable matrix
    1.
    发明授权
    Artificial pancreatic perfusion device with reseedable matrix 失效
    人造胰腺灌注装置与可重复使用的基质

    公开(公告)号：US5116493A

    公开(公告)日：1992-05-26

    申请号：US674698

    申请日：1991-03-25

    申请人： William L. Chick ,  Randall G. Rupp

    发明人： William L. Chick ,  Randall G. Rupp

    IPC分类号： A61M1/36 ,  A61F2/02 ,  A61L27/04 ,  A61L27/14 ,  A61M1/16 ,  A61M1/34

    CPC分类号： A61L27/04 ,  A61F2/022 ,  A61L27/14 ,  A61M1/3472 ,  A61M1/3475 ,  A61M1/1678

    摘要： A device which serves as an artificial pancreas comprises a hollow fiber which is surrounded by islets of Langerhans enclosed in a housing. The islets are suspended in a semi-gel matrix or supporting material that is sufficiently viscous to physically support and maintain location of the islets about the hollow fiber, but sufficiently fluid to enable removal of the islet suspension from the device, followed by reseeding or replacement with fresh islet suspension. The matrix in which the islets are suspended preferably comprises calcium sodium alginate. The semi-gel supporting material also enables long term maintenance of islet cells in in vitro culture.

    摘要翻译： 用作人造胰腺的装置包括被包围在壳体中的朗格汉斯小岛包围的中空纤维。 胰岛悬浮在足够粘稠的半凝胶基质或支撑材料中，以物理地支撑和保持胰岛在中空纤维周围的位置，但足够流体以使胰岛悬浮液能够从装置中移除，随后进行再次接种或更换 与新鲜的胰岛悬浮。 其中悬挂胰岛的基质优选包含藻酸钙钠。 半凝胶支持材料还能够在体外培养中长期维持胰岛细胞。

    Artificial pancreatic perfusion device with temperature sensitive matrix
    2.
    发明授权
    Artificial pancreatic perfusion device with temperature sensitive matrix 失效
    人造胰腺灌注装置，具有温敏矩阵

    公开(公告)号：US5116494A

    公开(公告)日：1992-05-26

    申请号：US674787

    申请日：1991-03-25

    申请人： William L. Chick ,  Susan J. Sullivan

    发明人： William L. Chick ,  Susan J. Sullivan

    IPC分类号： A61M1/36 ,  A61F2/02 ,  A61K35/39 ,  A61L27/04 ,  A61L27/14 ,  A61M1/16 ,  A61M1/34 ,  A61P3/08

    CPC分类号： A61L27/04 ,  A61F2/022 ,  A61L27/14 ,  A61M1/3472 ,  A61M1/3475 ,  A61M1/3489 ,  A61M1/3493 ,  A61M1/1678

    摘要： A device which serves as an artificial pancreas comprises a hollow fiber which is surrounded by islets of Langerhans enclosed in a housing. The islets are suspended in a temperature sensitive matrix which is sufficiently viscous to support islets at a temperature below about 45.degree. C. and sufficiently fluid to enable removal of islet suspension at a temperature above about 45.degree. C. A warm (e.g., 48.degree. to 50.degree. C.) solution may be flushed through the device to change the physical state of the temperature sensitive matrix from a semi-solid state to a liquified semi-gel state. The temperature sensitive supporting material also enables long term maintenance of islet cells in in vitro culture.

    摘要翻译： 用作人造胰腺的装置包括被包围在壳体中的朗格汉斯小岛包围的中空纤维。 将胰岛悬浮在温度敏感的基质中，其具有足够的粘稠性，以在低于约45℃的温度下支撑胰岛，并且足够的流体使得能够在高于约45℃的温度下除去胰岛悬浮液。温热（例如48℃ 至50℃）溶液可以冲洗穿过该装置，以将温度敏感基体的物理状态从半固态改变为液化的半凝胶状态。 温度敏感的支持材料还能够在体外培养中长期维持胰岛细胞。

    Methods and device for detecting and quantifying substances in body fluids
    3.
    发明授权
    Methods and device for detecting and quantifying substances in body fluids 失效
    用于检测和定量体液中物质的方法和装置

    公开(公告)号：US06040194A

    公开(公告)日：2000-03-21

    申请号：US467915

    申请日：1995-06-06

    申请人： William L. Chick ,  David E. Wolf ,  Richard A. Cardullo

    发明人： William L. Chick ,  David E. Wolf ,  Richard A. Cardullo

    IPC分类号： A61K49/00 ,  A61B5/00 ,  A61B8/00 ,  G01N21/64

    CPC分类号： A61K49/0004 ,  A61K49/0006 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/815 ,  Y10T436/144444

    摘要： An in vivo method and apparatus for detecting an analyte in an individual. A sensor that includes a fluorescence reagent is placed in communication with the body fluids of the individual suspected of containing the analyte in such a way that once in place said sensor does not exit the skin of the individual. The sensor is configured to retain the fluorescence reagent while allowing analyte to diffuse into and out of said sensor. The sensor is illuminated transdermally, and the fluorescence from the fluorescence reagent associated with the presence of the analyte is measured.

    摘要翻译： 用于检测个体中的分析物的体内方法和装置。 将包含荧光试剂的传感器放置成与怀疑含有分析物的个体的体液连通，使得一旦就位，所述传感器不会离开个体的皮肤。 传感器被配置为保持荧光试剂，同时允许分析物扩散进入和离开所述传感器。 传感器经皮照射，并测量与分析物存在相关的荧光试剂的荧光。

    Microcapsules and composite microreactors for immunoisolation of cells
    5.
    发明授权
    Microcapsules and composite microreactors for immunoisolation of cells 失效

    公开(公告)号：US6126936A

    公开(公告)日：2000-10-03

    申请号：US402209

    申请日：1995-03-10

    申请人： Robert P. Lanza ,  Willem M. Kuhtreiber ,  William L. Chick

    发明人： Robert P. Lanza ,  Willem M. Kuhtreiber ,  William L. Chick

    IPC分类号： C12N11/00 ,  A61K9/16 ,  A61K9/50 ,  A61K35/12 ,  A61K35/39 ,  A61K45/00 ,  A61K47/30 ,  A61K48/00 ,  C12N5/00 ,  C12N11/04 ,  C12N11/10

    CPC分类号： C12N5/0012 ,  A61K48/00 ,  C12N11/04 ,  C12N2533/32 ,  C12N2533/74

    摘要： Microcapsules and composite microreactors are prepared that immunoisolate living cells such as islet cells or genetically engineered cells. A reduced volume microcapsule is formed by coating a gel matrix particle with a polyamino acid of 15,000 daltons or less molecular weight to reduce volume of the particle by at least 30% as compared to volume prior to coating. A composite microreactor includes the microcapsule containing cell embedded in a gel matrix and provides a molecular weight cutoff that prevents molecules larger than about 400,000 daltons from containing the living cell. A double composite microreactor includes an internal particle that includes an internal particle gel matrix containing a living cell and having a coating, a particle that includes the internal particle embedded in a particle gel matrix and a coating, and a gel super matrix in which the particle is embedded. At least one of the coatings is a volume reducing coating of polyamino acid of 15,000 daltons or less molecular weight. The gel matrices may be alginate, the polyamino acid may be polylysine or polyornithine, and at least one of the gel matrices or coatings may be treated by aging for between 2 hours and 14 days. The internal particle, particle and composite microreactor may have diameters respectively between 50 and 700 microns, 400 and 800 microns and 300 and 1500 microns. to be implanted so there is little or no need for immunosuppressant or antifibrotic drugs. A reduced volume microcapsule is formed by coating a gel matrix particle with a polyamino acid of 15,000 daltons or less molecular weight to reduce volume of the particle by at least 30% as compared to volume prior to coating. A composite microreactor is provided by embedding the microcapsule when containing a living cell in a gel matrix to obtain a molecular weight cutoff that prevents molecules larger than about 400,000 daltons from contacting the living cell. A double composite microreactor is formed containing an internal particle including an internal particle gel matrix containing a living cell and having a coating, a particle including the internal particle embedded in a particle gel matrix having a coating, and a gel super matrix in which the particle is embedded. At least one of the coatings is a volume reducing coating of polyamino acid of 15,000 daltons or less molecular weight. The gel matrices may be alginate, the polyamino acid may be polylysine or polyornithine, and at least one of the gel matrices or coatings may be treated by aging for between 2 hours and 14 days. The internal particle, particle and composite microreactor may have diameters respectively between 50 and 700 microns, 400 and 800 microns and 300 and 1500 microns.