GREEN PROCESS AND COMPOSITIONS FOR PRODUCING POLY(5HV) AND 5 CARBON CHEMICALS
    2.
    发明申请
    GREEN PROCESS AND COMPOSITIONS FOR PRODUCING POLY(5HV) AND 5 CARBON CHEMICALS 有权
    用于生产聚(5HV)和5碳化合物的绿色方法和组合物

    公开(公告)号:US20100168481A1

    公开(公告)日:2010-07-01

    申请号:US12637706

    申请日:2009-12-14

    摘要: Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminopentanoate (5AP), 5-hydroxyvalerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is E. coli. The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate).

    摘要翻译: 提供了用于生产聚羟基链烷酸酯的重组宿主和从可再生碳底物生产聚羟基链烷酸酯的方法。 还提供了产生5种碳化学物质的某些重组宿主,如5-氨基戊酸(5AP),5-羟基戊酸酯(5HV),戊二酸酯和1,5-戊二醇(PDO)。 一个实施方案提供了表达编码选自聚羟基链烷酸酯合酶和5-羟基戊酸酯-CoA(5HV-CoA)转移酶的异源酶的基因的重组宿主,其中所述宿主产生含有5-羟基戊酸酯的聚合物。 优选地,宿主表达聚羟基链烷酸酯合成酶和5HV-CoA转移酶。 宿主可以是原核生物或真核生物。 优选的原核宿主是大肠杆菌。 由重组宿主产生的聚合物可以是5-羟基戊酸酯的均聚物或共聚物。 优选的共聚物是聚(3-羟基丁酸酯 - 共-5-羟基戊酸酯)。