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    APPARATUS AND METHOD FOR PRODUCING HIGH PURITY X-CHROMOSOME BEARING AND/OR Y-CHROMOSOME BEARING POPULATIONS OF SPERMATOZOA
    6.
    发明申请
    APPARATUS AND METHOD FOR PRODUCING HIGH PURITY X-CHROMOSOME BEARING AND/OR Y-CHROMOSOME BEARING POPULATIONS OF SPERMATOZOA 审中-公开
    用于生产高倍X轴染色体轴承和/或Y型染色体轴承的装置和方法

    公开(公告)号：US20150152497A1

    公开(公告)日：2015-06-04

    申请号：US14581536

    申请日：2014-12-23

    申请人： XY, LLC

    发明人： Kenneth M. Evans ,  Erik B. van Munster

    IPC分类号： C12Q1/68 ,  G01N15/14

    CPC分类号： C12Q1/6879 ,  A61D19/00 ,  B03B9/00 ,  C12N5/061 ,  C12N5/0612 ,  G01F17/00 ,  G01N15/14 ,  G01N15/147 ,  G01N21/17 ,  G01N2015/149

    摘要： A flow cytometer apparatus and method for differentiating X-chromosome bearing sperm and Y-chromosome bearing sperm having differing amounts of a light emission material coupled to nuclear their DNA. The apparatus may include an irradiation source which generates an irradiation beam responsive to the light emission material coupled to stained sperm cells, optics to focus the irradiation beam to a beam pattern having a height about equal to the length of said sperm cells along the longitudinal axis to about three times the length of said sperm cells along the longitudinal axis, and a detector responsive to said light emitted from said light emission material in response to the irradiation beam. The method may include the steps of establishing a fluid stream, producing an irradiation beam directed at the fluid stream, shaping the irradiation beam to have a beam height between about equal to the length of said sperm cells along the longitudinal axis and about three times the length of said sperm cells along the longitudinal axis at the fluid stream, and detecting emissions from sperm cells passing through the beam pattern.

    摘要翻译： 一种用于区分携带X染色体的精子和携带Y染色体的精子的流式细胞仪装置和方法，其具有与核DNA连接的发光材料量不同的量。 该装置可以包括照射源，其产生响应于与染色的精细胞耦合的发光材料的照射束，光学器件将照射束聚焦到具有等于所述精细胞的长度的波束图案，所述高度大约等于所述精细胞的长度沿着纵轴 到所述精子细胞沿着纵向轴线的长度的约三倍，以及响应于照射束响应于从所述发光材料发射的所述光的检测器。 该方法可以包括以下步骤：建立流体流，产生指向流体流的照射束，使照射束成形，使束高度大约等于所述精子细胞沿着纵轴的长度，并且大约是三倍 所述精子细胞沿流体流的纵向轴线的长度，以及检测通过束图案的精子细胞的排出。

    EFFICIENT HAPLOID CELL SORTING FLOW CYTOMETER SYSTEMS
    7.
    发明申请
    EFFICIENT HAPLOID CELL SORTING FLOW CYTOMETER SYSTEMS 审中-公开
    有效的HAPLOID细胞分流流式细胞仪系统

    公开(公告)号：US20130224843A1

    公开(公告)日：2013-08-29

    申请号：US13764548

    申请日：2013-02-11

    申请人： XY, LLC

    发明人： Kenneth M. Evans ,  Thomas B. Gillilgan

    IPC分类号： C12Q3/00

    CPC分类号： C12Q3/00 ,  C12N5/0612 ,  G01N15/147 ,  G01N15/1475 ,  G01N33/5005 ,  G01N2015/149 ,  Y10T436/12

    摘要： A flow cytometry system (1) for sorting haploid cells, specifically irradiatable sperm cells, with an intermittingly punctuated radiation emitter (56). Embodiments include a beam manipulator (21) and even split radiation beams directed to multiple nozzles (5). Differentiation of sperm characteristics with increased resolution may efficiently allow differentiated sperm cells to be separated higher speeds and even into subpopulations having higher purity.

    摘要翻译： 一种流式细胞术系统（1），用于将单倍体细胞（特别是可照射的精子细胞）与中间标记的辐射发射器（56）分离。 实施例包括光束操纵器（21）以及指向多个喷嘴（5）的均匀的分束辐射束。 精子特征与分辨率的分化可以有效地使分化精子细胞被分离得更高，甚至可以分离出具有较高纯度的亚群。