公开(公告)号：US07790450B2

公开(公告)日：2010-09-07

申请号：US12037117

申请日：2008-02-26

申请人： Yasuko Fujita ,  Hideki Tohda ,  Yuko Hama

发明人： Yasuko Fujita ,  Hideki Tohda ,  Yuko Hama

IPC分类号： C12N15/63 ,  C12N15/85 ,  C12P21/00

CPC分类号： C12N15/815 ,  C12N15/81

摘要： To provide a heterologous protein expression system which enables the fission yeast Schizosaccharomyces pombe as a host to produce of a heterologous protein which is almost impossible to express, or if possible, produced at low levels, under control of conventional promoters.The present invention provides an expression for use in the fission yeast Schizosaccharomyces pombe, a transformant carrying the expression vector, a method for producing a heterologous protein using the transformant, in particular a method for producing a heterologous protein in which a heat shock protein gene promoter in Schizosaccharomyces pombe is used to regulate gene expression by application of a specific form of stress, whereby the timing of the production of the desired heterologous protein can be controlled.

摘要翻译： 提供异源蛋白质表达系统，其能够使裂殖酵母裂殖酵母作为宿主产生异源蛋白，其几乎不可能在常规启动子的控制下表达，或者如果可能的话，以低水平产生。 本发明提供了用于裂殖酵母裂殖酵母中的表达，携带表达载体的转化体，使用该转化体产生异源蛋白质的方法，特别是制备异源蛋白质的方法，其中热休克蛋白基因启动子 粟酒裂殖酵母（Schizosaccharomyces pombe）用于通过施加特定形式的胁迫来调节基因表达，从而可以控制所需异源蛋白质的产生时间。

公开(公告)号：US06417327B1

公开(公告)日：2002-07-09

申请号：US09537817

申请日：2000-03-22

申请人： Shuichi Oka ,  Yoshimitsu Yamazaki ,  Toru Imamura ,  Yasuko Fujita ,  Saori Yamamoto ,  Yukiko Okita ,  Kazuo Ozawa ,  Reiko Akakura ,  Chikako Ito

发明人： Shuichi Oka ,  Yoshimitsu Yamazaki ,  Toru Imamura ,  Yasuko Fujita ,  Saori Yamamoto ,  Yukiko Okita ,  Kazuo Ozawa ,  Reiko Akakura ,  Chikako Ito

IPC分类号： C07K1450

CPC分类号： C07K14/50

摘要： The present invention provides a peptide partially comprising an amino acid sequence of FGF-5 or FGF-5S (i.e., the alternatively spliced form of FGF-5), the peptide being capable of controlling physiological function(s) of FGF-5. A pharmaceutical composition containing the peptide of the invention as an active component may regulate the physiological function(s) of FGF-5 such as regulation of development or growth of a head or body hair, regulation of nutrition or function of a cranial nerve system, regulation of platelets, and/or promotion or inhibition of proliferation or differentiation of a vascular endothelial cell.

摘要翻译： 本发明提供了部分包含FGF-5或FGF-5S的氨基酸序列（即FGF-5的可变剪接形式）的肽，该肽能够控制FGF-5的生理功能。 含有作为活性成分的本发明的肽的药物组合物可以调节FGF-5的生理功能，例如调节头部或体毛的发育或生长，调节营养或颅神经系统的功能， 调节血小板，和/或促进或抑制血管内皮细胞的增殖或分化。

公开(公告)号：US09017969B2

公开(公告)日：2015-04-28

申请号：US13409558

申请日：2012-03-01

申请人： Hiroko Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

发明人： Hiroko Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

IPC分类号： C12P21/00 ,  C12N1/19 ,  C12N1/16 ,  C12N9/10 ,  C12N9/34 ,  C12N9/60 ,  C07K14/47 ,  C12N9/24 ,  C12N15/09

CPC分类号： C12P21/005 ,  C07K14/4726 ,  C07K2319/03 ,  C12N1/16 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2428 ,  C12N9/2488 ,  C12N9/60 ,  C12N15/09 ,  C12Y204/01109 ,  C12Y204/01132 ,  C12Y204/01255 ,  C12Y302/01003 ,  C12Y302/01113 ,  C12Y304/21048 ,  C12Y304/23025

摘要： The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and further having an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of Man5GlcNAc2; and a method for producing glycoproteins using them.

摘要翻译： 本发明提供：遗传修饰的酵母，例如具有产生Man5GlcNAc2的N-连接糖链的能力的突变型酵母和产生O-连接的糖链的能力降低的突变体酵母，具有产生Man5GlcNAc2的N-连接糖链的能力的突变体酵母 并且还具有产生GlcNAc1Man5GlcNAc2的N-连接的糖链的能力，以及具有增强的产生和分泌蛋白质的能力和产生Man5GlcNAc2的N-连接的糖链的能力的突变体酵母; 以及使用它们生产糖蛋白的方法。

公开(公告)号：US20130171692A1

公开(公告)日：2013-07-04

申请号：US13409558

申请日：2012-03-01

申请人： Hiroke Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

发明人： Hiroke Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

IPC分类号： C12N1/19 ,  C12P21/00

CPC分类号： C12P21/005 ,  C07K14/4726 ,  C07K2319/03 ,  C12N1/16 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2428 ,  C12N9/2488 ,  C12N9/60 ,  C12N15/09 ,  C12Y204/01109 ,  C12Y204/01132 ,  C12Y204/01255 ,  C12Y302/01003 ,  C12Y302/01113 ,  C12Y304/21048 ,  C12Y304/23025

摘要： The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and further having an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of Man5GlcNAc2; and a method for producing glycoproteins using them.

摘要翻译： 本发明提供：遗传修饰的酵母，例如具有产生Man5GlcNAc2的N-连接糖链的能力的突变型酵母和产生O-连接的糖链的能力降低的突变体酵母，具有产生Man5GlcNAc2的N-连接糖链的能力的突变体酵母 并且还具有产生GlcNAc1Man5GlcNAc2的N-连接的糖链的能力，以及具有增强的产生和分泌蛋白质的能力和产生Man5GlcNAc2的N-连接的糖链的能力的突变体酵母; 以及使用它们生产糖蛋白的方法。