公开(公告)号：US09017969B2

公开(公告)日：2015-04-28

申请号：US13409558

申请日：2012-03-01

申请人： Hiroko Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

发明人： Hiroko Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

IPC分类号： C12P21/00 ,  C12N1/19 ,  C12N1/16 ,  C12N9/10 ,  C12N9/34 ,  C12N9/60 ,  C07K14/47 ,  C12N9/24 ,  C12N15/09

CPC分类号： C12P21/005 ,  C07K14/4726 ,  C07K2319/03 ,  C12N1/16 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2428 ,  C12N9/2488 ,  C12N9/60 ,  C12N15/09 ,  C12Y204/01109 ,  C12Y204/01132 ,  C12Y204/01255 ,  C12Y302/01003 ,  C12Y302/01113 ,  C12Y304/21048 ,  C12Y304/23025

摘要： The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and further having an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of Man5GlcNAc2; and a method for producing glycoproteins using them.

摘要翻译： 本发明提供：遗传修饰的酵母，例如具有产生Man5GlcNAc2的N-连接糖链的能力的突变型酵母和产生O-连接的糖链的能力降低的突变体酵母，具有产生Man5GlcNAc2的N-连接糖链的能力的突变体酵母 并且还具有产生GlcNAc1Man5GlcNAc2的N-连接的糖链的能力，以及具有增强的产生和分泌蛋白质的能力和产生Man5GlcNAc2的N-连接的糖链的能力的突变体酵母; 以及使用它们生产糖蛋白的方法。

公开(公告)号：US20110189723A1

公开(公告)日：2011-08-04

申请号：US13060531

申请日：2009-09-02

申请人： Shohei Yamamura ,  Shouki Yatsushiro ,  Masatoshi Kataoka

发明人： Shohei Yamamura ,  Shouki Yatsushiro ,  Masatoshi Kataoka

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： C12Q1/04 ,  G01N33/5091 ,  G01N33/56905

摘要： Disclosed is a detection method for detecting a specific cell in a sample containing multiple cells including the specific cell. Specifically disclosed is a detection method for detecting a specific cell in a sample containing multiple cells including the specific cell, which comprises the following steps (1) and (2): (1) retaining cells contained in the sample on a microarray chip which comprises multiple microchambers, wherein each of the microchambers can contain multiple cells; and (2) confirming the presence or absence of the specific cell in the cells retained on the microarray chip.

摘要翻译： 公开了一种用于检测包含特定细胞的多个细胞的样品中的特定细胞的检测方法。 具体公开了一种用于检测包含特定细胞的多个细胞的样品中的特定细胞的检测方法，其包括以下步骤（1）和（2）：（1）将包含在样品中的细胞保留在微阵列芯片上，所述芯片包含 多个微室，其中每个微室可以包含多个细胞; 和（2）确认在微阵列芯片中保留的细胞中特异性细胞的存在或不存在。

公开(公告)号：US09249445B2

公开(公告)日：2016-02-02

申请号：US13060531

申请日：2009-09-02

申请人： Shohei Yamamura ,  Shouki Yatsushiro ,  Masatoshi Kataoka

发明人： Shohei Yamamura ,  Shouki Yatsushiro ,  Masatoshi Kataoka

IPC分类号： C12Q1/04 ,  G01N33/50 ,  G01N33/569

CPC分类号： C12Q1/04 ,  G01N33/5091 ,  G01N33/56905

摘要： Disclosed is a detection method for detecting a specific cell in a sample containing multiple cells including the specific cell. Specifically disclosed is a detection method for detecting a specific cell in a sample containing multiple cells including the specific cell, which comprises the following steps (1) and (2): (1) retaining cells contained in the sample on a microarray chip which comprises multiple microchambers, wherein each of the microchambers can contain multiple cells; and (2) confirming the presence or absence of the specific cell in the cells retained on the microarray chip.

摘要翻译： 公开了一种用于检测包含特定细胞的多个细胞的样品中的特定细胞的检测方法。 具体公开了一种用于检测包含特定细胞的多个细胞的样品中的特定细胞的检测方法，其包括以下步骤（1）和（2）：（1）将包含在样品中的细胞保留在微阵列芯片上，所述芯片包含 多个微室，其中每个微室可以包含多个细胞; 和（2）确认在微阵列芯片中保留的细胞中特异性细胞的存在或不存在。

公开(公告)号：US20130171692A1

公开(公告)日：2013-07-04

申请号：US13409558

申请日：2012-03-01

申请人： Hiroke Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

发明人： Hiroke Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

IPC分类号： C12N1/19 ,  C12P21/00

CPC分类号： C12P21/005 ,  C07K14/4726 ,  C07K2319/03 ,  C12N1/16 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2428 ,  C12N9/2488 ,  C12N9/60 ,  C12N15/09 ,  C12Y204/01109 ,  C12Y204/01132 ,  C12Y204/01255 ,  C12Y302/01003 ,  C12Y302/01113 ,  C12Y304/21048 ,  C12Y304/23025

摘要： The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and further having an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of Man5GlcNAc2; and a method for producing glycoproteins using them.

摘要翻译： 本发明提供：遗传修饰的酵母，例如具有产生Man5GlcNAc2的N-连接糖链的能力的突变型酵母和产生O-连接的糖链的能力降低的突变体酵母，具有产生Man5GlcNAc2的N-连接糖链的能力的突变体酵母 并且还具有产生GlcNAc1Man5GlcNAc2的N-连接的糖链的能力，以及具有增强的产生和分泌蛋白质的能力和产生Man5GlcNAc2的N-连接的糖链的能力的突变体酵母; 以及使用它们生产糖蛋白的方法。