公开(公告)号：US20050096520A1

公开(公告)日：2005-05-05

申请号：US10982101

申请日：2004-11-04

申请人： Yasunori Maekawa ,  Kennichi Sawa ,  Toshiyuki Sato ,  Seiki Okada ,  Kanako Nagaoka ,  Kaoru Asano

发明人： Yasunori Maekawa ,  Kennichi Sawa ,  Toshiyuki Sato ,  Seiki Okada ,  Kanako Nagaoka ,  Kaoru Asano

IPC分类号： A61B5/00 ,  A61B5/145 ,  A61B5/1468 ,  A61B10/00

CPC分类号： A61B5/14546 ,  A61B5/14514 ,  A61B5/14532 ,  A61B2010/008

摘要： Extraction devices for extracting analyte through the skin of a living body are disclosed that includes first and second extraction units which are placed on the skin and in which analyte is extracted; electrode unit placed on the skin; and a power source unit for outputting a first current flowing through the electrode unit, the living body, and the first extraction unit, and a second current flowing through the electrode unit, the living body, and the second extraction unit.

摘要翻译： 公开了通过生物体的皮肤提取分析物的提取装置，其包括放置在皮肤上并且分析物被提取的第一和第二提取单元; 放置在皮肤上的电极单元; 以及电源单元，用于输出流过电极单元，生物体和第一提取单元的第一电流和流过电极单元，生物体和第二提取单元的第二电流。

公开(公告)号：US20110040166A1

公开(公告)日：2011-02-17

申请号：US12909577

申请日：2010-10-21

申请人： Seiki Okada ,  Yasunori Maekawa ,  Kennichi Sawa ,  Toshiyuki Sato ,  Kanako Nagaoka ,  Kei Hagino

发明人： Seiki Okada ,  Yasunori Maekawa ,  Kennichi Sawa ,  Toshiyuki Sato ,  Kanako Nagaoka ,  Kei Hagino

IPC分类号： A61B5/145

CPC分类号： A61B5/681 ,  A61B5/14514 ,  A61B5/14532 ,  A61M2037/0007 ,  A61N1/30 ,  A61N1/306

摘要： An extracting kits for extracting analyte through the skin of a living body are disclosed that comprises an inserting device comprising needles for forming a plurality of extraction holes in the skin through the stratum corneum but not reaching the subcutaneous tissue and an extracting device for extracting the analyte into a liquid supplied into the extraction holes by supplying the liquid into the plurality of extraction holes. An extracting device and extracting method are also described.

摘要翻译： 公开了一种用于通过生物体的皮肤提取分析物的提取试剂盒，其包括插入装置，所述插入装置包括用于通过角质层在皮肤中形成多个提取孔但不到达皮下组织的针，以及用于提取分析物的提取装置 通过将液体供给到多个提取孔中而供入到提取孔中的液体。 还描述了提取装置和提取方法。

公开(公告)号：US20050177037A1

公开(公告)日：2005-08-11

申请号：US11031722

申请日：2005-01-07

申请人： Seiki Okada ,  Yasunori Maekawa ,  Kennichi Sawa ,  Toshiyuki Sato ,  Kanako Nagaoka ,  Kei Hagino

发明人： Seiki Okada ,  Yasunori Maekawa ,  Kennichi Sawa ,  Toshiyuki Sato ,  Kanako Nagaoka ,  Kei Hagino

IPC分类号： A61B5/00 ,  A61N1/30 ,  A61B5/05

CPC分类号： A61B5/681 ,  A61B5/14514 ,  A61B5/14532 ,  A61M2037/0007 ,  A61N1/30 ,  A61N1/306

摘要： An extracting kits for extracting analyte through the skin of a living body are disclosed that comprises an inserting device comprising needles for forming a plurality of extraction holes in the skin through the stratum corneum but not reaching the subcutaneous tissue and an extracting device for extracting the analyte into a liquid supplied into the extraction holes by supplying the liquid into the plurality of extraction holes. An extracting device and extracting method are also described.

摘要翻译： 公开了一种用于通过生物体的皮肤提取分析物的提取试剂盒，其包括插入装置，所述插入装置包括用于通过角质层在皮肤中形成多个提取孔但不到达皮下组织的针，以及用于提取分析物的提取装置 通过将液体供给到多个提取孔中而供入到提取孔中的液体。 还描述了提取装置和提取方法。

公开(公告)号：US20050169799A1

公开(公告)日：2005-08-04

申请号：US11049599

申请日：2005-02-02

申请人： Kennichi Sawa ,  Yasunori Maekawa ,  Toshiyuki Sato ,  Kanako Nagaoka ,  Seiki Okada ,  Kei Hagino

发明人： Kennichi Sawa ,  Yasunori Maekawa ,  Toshiyuki Sato ,  Kanako Nagaoka ,  Seiki Okada ,  Kei Hagino

IPC分类号： A61B5/00 ,  G01N31/00 ,  C12M3/00

CPC分类号： A61B5/14532 ,  A61B5/14514 ,  A61B5/1459

摘要： An analyzer for extracting and analyzing an analyte from a subject is disclosed that comprises a reservoir capable of holding a liquid for holding the analyte extracted from the subject; and a liquid supplying mechanism for holding a liquid to be supplied to the reservoir and supplying the liquid to the reservoir. A cartridge, cartridge kit, and analyzing method are also described.

摘要翻译： 公开了一种用于提取和分析来自受试者的分析物的分析器，其包括能够容纳用于保持从被检体提取的分析物的液体的储存器; 以及液体供给机构，用于保持要供应到储存器的液体并将液体供应到储存器。 还描述了墨盒，墨盒套件和分析方法。

公开(公告)号：US20070278154A1

公开(公告)日：2007-12-06

申请号：US11753943

申请日：2007-05-25

申请人： Kanako Nagaoka ,  Masaki Ishisaka

发明人： Kanako Nagaoka ,  Masaki Ishisaka

IPC分类号： B01D61/00

CPC分类号： C12M47/02

摘要： A cell processing method for processing and collecting cells in a cell suspension fluid with reagent comprising: filtering a cell suspension fluid using a membrane filter; processing the cells ion the membrane filter with reagent; supplying a collection fluid to produce a shearing force that acts on the cells processed with reagent so as to separate the cells on the membrane filter into the collection fluid; and recovering the collection fluid that retains the cells separated from the membrane filter.

摘要翻译： 一种用于用试剂处理和收集细胞悬浮液中的细胞的细胞处理方法，包括：使用膜过滤器过滤细胞悬浮液; 用试剂处理细胞离子膜过滤器; 供应收集流体以产生作用于用试剂处理的细胞的剪切力，以将膜过滤器上的细胞分离成收集流体; 并回收保留从膜过滤器分离的细胞的收集液。

公开(公告)号：US20110014646A1

公开(公告)日：2011-01-20

申请号：US12892257

申请日：2010-09-28

申请人： Masakazu Fukuda ,  Kanako Nagaoka ,  Masaki Ishisaka

发明人： Masakazu Fukuda ,  Kanako Nagaoka ,  Masaki Ishisaka

IPC分类号： C12Q1/06 ,  C12M1/34

CPC分类号： G01N1/34 ,  G01N15/0618 ,  G01N15/147 ,  G01N15/1475 ,  G01N35/1095 ,  G01N2015/1006 ,  G01N2015/1493 ,  G01N2035/00475

摘要： A sample preparation apparatus comprising: a detector for detecting a predetermined cell included in a biological sample; a sample preparation section for preparing a measurement sample from the biological sample and a predetermined reagent; and a controller configured to generate, based on a detection result by the detector, concentration information reflecting a concentration of the predetermined cell in the biological sample and to control, based on the concentration information, the amount of the biological sample supplied to the sample preparation section.

摘要翻译： 一种样品制备装置，包括：用于检测生物样品中包含的预定细胞的检测器; 用于从生物样品和预定试剂制备测量样品的样品制备部分; 以及控制器，被配置为基于检测器的检测结果生成反映生物样品中预定细胞浓度的浓度信息，并且基于浓度信息来控制提供给样品制备物的生物样品的量 部分。

公开(公告)号：US08189177B2

公开(公告)日：2012-05-29

申请号：US12892257

申请日：2010-09-28

申请人： Masakazu Fukuda ,  Kanako Nagaoka ,  Masaki Ishisaka

发明人： Masakazu Fukuda ,  Kanako Nagaoka ,  Masaki Ishisaka

IPC分类号： G01N1/00

CPC分类号： G01N1/34 ,  G01N15/0618 ,  G01N15/147 ,  G01N15/1475 ,  G01N35/1095 ,  G01N2015/1006 ,  G01N2015/1493 ,  G01N2035/00475

摘要： A sample preparation apparatus comprising: a detector for detecting a predetermined cell included in a biological sample; a sample preparation section for preparing a measurement sample from the biological sample and a predetermined reagent; and a controller configured to generate, based on a detection result by the detector, concentration information reflecting a concentration of the predetermined cell in the biological sample and to control, based on the concentration information, the amount of the biological sample supplied to the sample preparation section.

摘要翻译： 一种样品制备装置，包括：用于检测生物样品中包含的预定细胞的检测器; 用于从生物样品和预定试剂制备测量样品的样品制备部分; 以及控制器，被配置为基于检测器的检测结果生成反映生物样品中预定细胞浓度的浓度信息，并且基于浓度信息来控制提供给样品制备物的生物样品的量 部分。

公开(公告)号：US20110136131A1

公开(公告)日：2011-06-09

申请号：US11628063

申请日：2005-05-27

申请人： Satoshi Nakayama ,  Kanako Nagaoka ,  Hironori Kobayashi ,  Hideki Ishihara

发明人： Satoshi Nakayama ,  Kanako Nagaoka ,  Hironori Kobayashi ,  Hideki Ishihara

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54306 ,  G01N33/573 ,  G01N33/57407 ,  G01N2333/91215

摘要： A method of measuring an enzyme activity, which comprises the steps of: (1) introducing a sample containing an enzyme to be measured into a column packed with a packing material comprising a water-insoluble support and an antibody which is immobilized onto the support and which recognizes the enzyme to be measured, to thereby capture the enzyme with the antibody; (2) introducing a substrate for the enzyme into the column to react the enzyme captured by the antibody with the substrate; and (3) detecting a product obtained by the reaction. The above method allows the rapid measurement of an enzyme activity with good reaction efficiency.

摘要翻译： 一种测量酶活性的方法，其包括以下步骤：（1）将含有待测酶的样品引入填充有包含水不溶性载体和固定在载体上的抗体的填充物的柱中， 其识别要测量的酶，从而用抗体捕获酶; （2）将酶的底物引入柱中以使由抗体捕获的酶与底物反应; 和（3）检测通过反应获得的产物。 上述方法允许快速测量具有良好反应效率的酶活性。